Association between Presence of RmpA, MrkA and MrkD Genes and Antibiotic Resistance in Clinical Klebsiella pneumoniae Isolates from Hospitals in Tehran, Iran

Bakhtiari, Ronak; Javadi, Ali; Aminzadeh, Malihe; Molaee-Aghaee, Ebrahim; Shaffaghat, Zahra

doi:10.18502/ijph.v50i5.6118

Cited by 11 publications

(12 citation statements)

References 19 publications

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“…During the 3 table, The results show the highest percentage of resistance to the antibiotic Ceftriaxone while the lowest percentage of resistance to the antibiotic appears Imipenem These results were similar to the study (Bakhtiari et al,2021) The study recorded the lowest percentage of resistance to the antibiotic appears Imipenem 20% and highest percentage of resistance to the antibiotic Ceftriaxone 60% While these results contradiction to the study (Mohamed et al,2018) The study recorded highest percentage of resistance to the antibiotic Amoxicillin -clavulanat and lowest percentage of resistance to the antibiotic appears Meropenem This difference is attributed to the places where the samples were isolated in addition…”

Section: Resultssupporting

confidence: 86%

Hypervirulent (hypermucoviscosity) Klebsiella pneumoniae isolated from clinical samples in Al-Diwaniyah Hospital

Al-Zayadi

Mayahi

2022

ijhs

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Klebsiella pneumoniae is not only one of the major pathogens acquired from hospital, but it is also one of the pathogens transmitted through urine samples that causes the liver Abscesses, blood poisoning, diarrhea. The study aimed to isolate the Hypervirulent Klebsiella pneumoniae (Hvkp) bacteria from clinical samples in Al-Diwaniyah city / Iraq. And evaluation of the efficiency of some antibiotics in inhibiting isolates. The study extended from January to mid-April. A total of 50 isolates identified as Klebsiella pneumonia using traditional biochemical tests, Of them, 13 isolates were hypervirulent, with a percentage of 26% of the studied isolates. A drug sensitivity test was conducted for 10 antibiotics and they were as follows (Amoxicillin – clavulanat, Cefotaxime, Ceftazidime, Ceftriaxone, Cefoxitin, Imipenem, Meropenem, Azteronam, Amikacin and Gentamicin) The ratio of resistance to an antibody Amoxicillin – clavulanat have reached 50% While the percentage of resistance to Cefotaxime 46% While the percentage of resistance to Ceftazidime 56% While the percentage of resistance Ceftriaxone 60% While the percentage of resistance to Cefoxitin, Imipenem, Meropenem, Azteronam, Amikacin and Gentamicin Straight (56% , 20% ,26% , 40% , 46%, 56%).

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Section: Resultssupporting

confidence: 86%

Hypervirulent (hypermucoviscosity) Klebsiella pneumoniae isolated from clinical samples in Al-Diwaniyah Hospital

Al-Zayadi

Mayahi

2022

ijhs

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“…It provides specificity for fimbrial binding to materials such as collagen, thus conferring the ability to adhere to urinary and respiratory cells as well as to materials such as catheters (Murphy & Clegg, 2012). The presence of this gene in 58.3% of the samples studied, including strains K. pneumoniae and E. coli, indicates that these isolates may have virulence potential and zoonotic capabilities, as they were identified in isolates from animal and human nosocomial infections in hospitals (Bakhtiari et al, 2021).…”

Section: Resultsmentioning

confidence: 92%

Virulence genes and antibiotic resistance assessment of the blaCTX-M-15 gene in ESBL-producing Escherichia coli and Klebsiella pneumoniae from poultry food chain and human clinical cases in Brazil

Pereira,

Cardozo,

Guastalli

et al. 2023

Sem. Ci. Agr.

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Brazil is one of the world’s largest exporters of chicken-derived products. Consumer concerns regarding food contamination by multidrug-resistant bacteria capable of causing diseases have increased steadily over the years as they pose a significant public health risk. This study aimed to characterize strains of beta-lactam-resistant Escherichia coli and Klebsiella pneumoniae isolates from the poultry production chain and human clinical samples (chicken cloaca, chicken meat, human feces). A total of 36 isolates were examined, including 28 Escherichia coli and 8 Klebsiella pneumoniae isolates carrying the blaCTX-M-15 gene. These isolates were obtained from chicken cloaca and meat from poultry farms and slaughterhouses, respectively, as well as from human fecal samples from a clinical laboratory. All the establishments were located in São Paulo, Brazil. The isolates were characterized for their virulence genes by polymerase chain reaction, antimicrobial resistance by the disc diffusion method, serotyping by an agglutination test, and pathogenicity by an in vivo day-old chicken mortality test. The isolates exhibited a high frequency of Escherichia coli associated virulence genes such as iutA, iss, hlyF, ompT and iroN. In addition, mrkD was detected in the isolates. All isolates exhibited resistance to at least three different antimicrobial classes, and 21.4% (n = 6) of the Escherichia coli strains exhibited high pathogenicity in the day-old chicken assay in vivo. These results indicate a potential increase in the blaCTX-M-15 gene associated with virulence genes and antimicrobial resistance in Escherichia coli and Klebsiella pneumoniae. Both of these Enterobacteriaceae can be found in poultry feces and possibly contaminate poultry products, thus posing a risk of infection to other animals and humans, raising an alert about the sanitary aspects of food production in Brazil.

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“…The reaction mixture contain 2 μl of each primer and 2 μl for DNA's template. The thermal program was optimized and executed on a master cycler (Eppendorf, Germany) as shown in the table below (5). Note: 62°C is used as the annealing temperature for mrkd gene while 57°Cused as annealing temperature for fimh gene .…”

Section: Detection Of Fimh and Mrkd Genes In Kpneumoniae Isolatesmentioning

confidence: 99%

“…The production of the extended-spectrum beta-lactamase (ESBL), one of the major causes of increased infection in hospitals, the alteration of the permeability barrier, the target site represented by penicillin binding protein, or the alteration of outer membrane protein are the main causes of K. pneumoniae resistance to antibiotics, including beta lactam antibiotics (5). Furthermore, K. pneumoniae has a large number of Efflux Pumps that discharge the antibiotic outdoors.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of fimH& mrkDgenes of strong biofilm producers & MDR Klebsiella pneumoniae

Jabar

Auhim

Hussein

2022

ijhs

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Klebsiella pneumoniae is an adaptable pathogen that forms biofilms on a variety of surfaces. This study's objective was to identify the presence of fimbrial genes (types 1 and 3) in K. pneumoniae strains isolated from various clinical sources based on their antibiotic resistance and ability to form biofilms. According to identification utilizing the vitek 2 technology and confirmation by molecular identification targeting the 16S rRNA gene with a particular primer, forty isolates were identified from clinical specimens. The vitek 2 compact system was utilized to evaluate the antibiotic susceptibility of all the isolates. The findings revealed a range of resistance percentages, including 52.5% for Penicillin, 40.5% for Trimethoprim/Sulfamethoxazole, 34.5% for Cephalosporins, 6.25 % for Fluoroquinolones, and 2.5% for each of Carbapenem, Aminoglycoside, Tetracycline, and Nitrofurantoin. The 96-well microtiter plate technique was utilized to generate biofilms. The results demonstrated that all 40 Klebsiella pneumoniae isolates (100%) produced potent biofilms. In order to identify the genes involved in biofilm formation (fimh & mrkd) and the genes responsible for adhesin in type 1& type 3 fimbriae using traditional PCR method, eleven isolates were chosen for molecular analysis that are powerful biofilm makers and MDR.

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Association between Presence of RmpA, MrkA and MrkD Genes and Antibiotic Resistance in Clinical Klebsiella pneumoniae Isolates from Hospitals in Tehran, Iran

Cited by 11 publications

References 19 publications

Hypervirulent (hypermucoviscosity) Klebsiella pneumoniae isolated from clinical samples in Al-Diwaniyah Hospital

Hypervirulent (hypermucoviscosity) Klebsiella pneumoniae isolated from clinical samples in Al-Diwaniyah Hospital

Virulence genes and antibiotic resistance assessment of the blaCTX-M-15 gene in ESBL-producing Escherichia coli and Klebsiella pneumoniae from poultry food chain and human clinical cases in Brazil

Molecular detection of fimH& mrkDgenes of strong biofilm producers & MDR Klebsiella pneumoniae

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