Purpose: The aim of this cross-sectional study was to assess the association between sleep status and myopia in children and adolescents.Methods: Stratified cluster sampling a total of 30,188 schoolchildren from 14 schools located in 6 streets (Xinxiang, Songgang, Shiyan, Fuyong, Shajing and Xinan) of Baoan District in Shenzhen, China. The demographic characteristics of schoolchildren, information of self-reported myopia and their sleep status in the last 1 month were collected through using a standardized questionnaire. χ2-test was used to compare differences in myopia rates among children and adolescents in different sleep status. Logistic regression models were used to analyze the association of sleep status with myopia in children and adolescents.Results: The self-reported myopia rates was 49.8% among children and adolescents in Shenzhen. In primary, junior high and senior high students, the myopia rates were 25.6%, 62.4%, and 75.7%, respectively. After controlling for confounders, the results of multiple logistic analysis showed that night sleep duration < 7h/d (OR = 2.02, 95%CI: 1.87-2.19), falling asleep at a fixed time (no) (OR = 1.14, 95%CI: 1.08-1.20), getting up at a fixed time in the morning (no) (OR = 1.28, 95%CI: 1.19-1.37), putting off sleeping on weekends ≥ 2 h/d (OR = 1.28, 95%CI: 1.17-1.41) and Putting off getting up on weekends ≥ 2 h/d were risk factors (OR = 1.23, 95%CI: 1.13-1.34). After stratified by grade, social jet lag was not associated with myopia among senior high students.Conclusion: Insufficient sleep duration was positively associated with self-reported myopia, irregular patterns of sleep and social jet lag increased the risk of myopia among schoolchildren. Myopia prevention and control among children and adolescents should not only ensure adequate sleep, but also develop a habit of regular sleep.