Association of B3GNT5 Polymorphisms with Susceptibility to ETEC F4ab/ac in the White Duroc × Erhualian Intercross and 15 Outbred Pig Breeds

Ouyang, Jing; Zeng, Weihong; Ren, Jun; Yan, Xueming; Zhang, Zhiyan; Yang, Ming; Han, Pengfei; Huang, Xiang; Ai, Huashui; Huang, Lusheng

doi:10.1007/s10528-011-9454-4

Cited by 3 publications

(2 citation statements)

References 32 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For ETEC F4, single nucleotide polymorphisms (SNPs) located on Mucin4 ( MUC4 ) [48], on Mucin 13 ( MUC13 ) [49, 50], Mucin 20 ( MUC20 ) [51], the transferrin receptor ( TFRC ) [52], tyrosine kinase non-receptor 2 ( ACK1 ) [53], UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 ( B3GNT5 ) [52] genes have been proposed as genetic markers for pig ETEC resistance/susceptibility. Goetstouwer et al [54] have recently proposed new SNPs located on the candidate region (chr13: 144810100-144993222) as new determinates for ETEC F4 susceptibility.…”

Section: Setting Of the Challenge Modelmentioning

confidence: 99%

Methodology and application of Escherichia coli F4 and F18 encoding infection models in post-weaning pigs

Luise

Lauridsen

Bosi

et al. 2019

J Animal Sci Biotechnol

View full text Add to dashboard Cite

The enterotoxigenic Escherichia coli (ETEC) expressing F4 and F18 fimbriae are the two main pathogens associated with post-weaning diarrhea (PWD) in piglets. The growing global concern regarding antimicrobial resistance (AMR) has encouraged research into the development of nutritional and feeding strategies as well as vaccination protocols in order to counteract the PWD due to ETEC. A valid approach to researching effective strategies is to implement piglet in vivo challenge models with ETEC infection. Thus, the proper application and standardization of ETEC F4 and F18 challenge models represent an urgent priority. The current review provides an overview regarding the current piglet ETEC F4 and F18 challenge models; it highlights the key points for setting the challenge protocols and the most important indicators which should be included in research studies to verify the effectiveness of the ETEC challenge. Based on the current review, it is recommended that the setting of the model correctly assesses the choice and preconditioning of pigs, and the timing and dosage of the ETEC inoculation. Furthermore, the evaluation of the ETEC challenge response should include both clinical parameters (such as the occurrence of diarrhea, rectal temperature and bacterial fecal shedding) and biomarkers for the specific expression of ETEC F4/F18 (such as antibody production, specific F4/F18 immunoglobulins (Igs), ETEC F4/F18 fecal enumeration and analysis of the F4/F18 receptors expression in the intestinal brush borders). On the basis of the review, the piglets’ response upon F4 or F18 inoculation differed in terms of the timing and intensity of the diarrhea development, on ETEC fecal shedding and in the piglets’ immunological antibody response. This information was considered to be relevant to correctly define the experimental protocol, the data recording and the sample collections. Appropriate challenge settings and evaluation of the response parameters will allow future research studies to comply with the replacement, reduction and refinement (3R) approach, and to be able to evaluate the efficiency of a given feeding, nutritional or vaccination intervention in order to combat ETEC infection.

show abstract

Section: Setting Of the Challenge Modelmentioning

confidence: 99%

Methodology and application of Escherichia coli F4 and F18 encoding infection models in post-weaning pigs

Luise

Lauridsen

Bosi

et al. 2019

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Previous studies revealed that the loci controlling F4ab/F4ac receptor is located on SSC13q41 (reviewed in [70]). Mutations in functional genes including HEG1 , ITGB5 [65], MUC4 [71,72], FUT1 [73,74], B3GNT5 [75,76], MUC20 [77], and MUC13 [18] were found to be associated with porcine susceptibility. Jacobsen et al [91] characterized 34 SNPs in five candidate genes within the ETEC F4ab/ac candidate region, but no obvious causative mutations were identified for E. coli F4ab/F4ac susceptibility.…”

Section: Genetic Control Of Porcine Resistance To Gram-negative Bacilmentioning

confidence: 99%

Immunogenomics for identification of disease resistance genes in pigs: a review focusing on Gram-negative bacilli

Song

Zhu

Chen

2012

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Over the past years, infectious disease has caused enormous economic loss in pig industry. Among the pathogens, gram negative bacteria not only cause inflammation, but also cause different diseases and make the pigs more susceptible to virus infection. Vaccination, medication and elimination of sick pigs are major strategies of controlling disease. Genetic methods, such as selection of disease resistance in the pig, have not been widely used. Recently, the completion of the porcine whole genome sequencing has provided powerful tools to identify the genome regions that harboring genes controlling disease or immunity. Immunogenomics, which combines DNA variations, transcriptome, immune response, and QTL mapping data to illustrate the interactions between pathogen and host immune system, will be an effective genomics tool for identification of disease resistance genes in pigs. These genes will be potential targets for disease resistance in breeding programs. This paper reviewed the progress of disease resistance study in the pig focusing on Gram-negative bacilli. Major porcine Gram-negative bacilli and diseases, suggested candidate genes/pathways against porcine Gram-negative bacilli, and distributions of QTLs for immune capacity on pig chromosomes were summarized. Some tools for immunogenomics research were described. We conclude that integration of sequencing, whole genome associations, functional genomics studies, and immune response information is necessary to illustrate molecular mechanisms and key genes in disease resistance.

show abstract

Resistance to ETEC F4/F18–mediated piglet diarrhoea: opening the gene black box

Sinha

Sahoo

Shrivastava

et al. 2019

Trop Anim Health Prod

View full text Add to dashboard Cite

Association of B3GNT5 Polymorphisms with Susceptibility to ETEC F4ab/ac in the White Duroc × Erhualian Intercross and 15 Outbred Pig Breeds

Cited by 3 publications

References 32 publications

Methodology and application of Escherichia coli F4 and F18 encoding infection models in post-weaning pigs

Methodology and application of Escherichia coli F4 and F18 encoding infection models in post-weaning pigs

Immunogenomics for identification of disease resistance genes in pigs: a review focusing on Gram-negative bacilli

Resistance to ETEC F4/F18–mediated piglet diarrhoea: opening the gene black box

Contact Info

Product

Resources

About