Association of Colony Morphology with Coenzyme Q10 Production and Its Enhancement from Rhizobium radiobacter T6102W by Addition of Isopentenyl Alcohol as a Precursor

Abstract

Rhizobium radiobacter T6102 was morphologically purified by the aniline blue agar plates to give two distinct colonies; white smooth mucoid colony (T6102W) and blue rough colony (T6102B). The coenzyme Q 10 (CoQ 10) was produced just by T6102W, showing 2.0 mg/g of CoQ 10 content, whereas the T6102B did not produce the CoQ 10. All of the used CoQ 10 biosynthetic precursors enhanced the CoQ 10 production by T6102W. Specifically, the supplementation of 0.75 mM isopentenyl alcohol improved the CoQ 10 concentration (19.9 mg/l) and content (2.4 mg/g) by 42% and 40%, respectively.

The Terpene Mini-Path, a New Promising Alternative for Terpenoids Bio-Production

“…Using a bacterium, different yeasts, and a filamentous fungus, Kawada et al [ 16 ] demonstrated that the addition of DMAOH, IOH, geraniol (GOH), or mixtures of IOH with either DMAOH or GOH at concentrations of 0.5 mM–1 mM, improves the ubiquinone content of the considered cells by 30 to 72% at the end of the fermentation process. The same was also demonstrated later on using Rhizobium radiobacter [ 17 ] with a cellular increase of 30% in ubiquinone content following the addition in the culture medium of 1 mM IOH. In a completely different type of experiment aiming at the time to discover the late enzymes (genes) of the MEP pathway, it was demonstrated that an insertion in the promotor region of the lytB gene of Synechocystis strain PCC 6803 caused impaired growth and a greenish-yellow phenotype instead of the dark blue-green normal one.…”

Enhancement of rice vinegar production by modified semi-continuous culture based on analysis of enzymatic kinetic

“…Hence, more coenzyme Q will release the inhibition and improve the production of acetic acid (Table 3). Five precursors of ubiquinone are selected as promoter of fermentation and developed in A. pasteurianus according to Seo et al's work [21]. We used both precursors for Co Q production associated with isoprenoid side chain and benzoquinone ring synthesis [22].…”

Integration of heterologous 4-hydroxybenzoic acid transport proteins in Rhodobacter sphaeroides for enhancement of coenzyme Q10production

“…2,5,[13][14][15] The specic productivity of CoQ 10 achieved by these metabolically engineered E. coli strains was very low and not comparable to the natural CoQ 10 producers such as R. sphaeroides, A. tumefaciens and R. radiobacter. 16,17 Thus, it seems particularly attractive and practical to directly genetically modify R. sphaeroides and improve upon its intrinsically high CoQ 10 titer.…”