Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Zhang, Xiao-Gang; Hong, Ji‐Yeon; Yan, Guijun; Wang, Y. F.; Li, Q. W.; Hu, Jianhong

doi:10.17221/8239-cjas

Cited by 45 publications

(36 citation statements)

References 26 publications

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“…Therefore, it is necessary to ensure optimal genetic sires selection (Zhang et al 2015) as well as semen processing including semen collection (Dzyuba et al 2015), dilution (Meamar et al 2016), slow cooling to 4-6°C (Kristan et al 2014), and equilibration (Beran et al 2013b) as well as cryopreservation maintaining spermatozoa fertilization capability (Andrabi 2007) evaluated objectively if possible (Simonik et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

Czech J. Anim. Sci.

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ABSTRACT:The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. Th e spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD,; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

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Section: Introductionmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

Czech J. Anim. Sci.

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“…comm.). This might be a consequence of pedigree, as Squires (2013) reported the poorest quality in the Friesian, Andalusian, and Saddlebreds; moreover, quite high inbreeding depression in this breed (Vostra-Vydrova et al 2016) might negatively interfere with reproduction (Roldan et al 2006) Even though the cryopreservation process was introduced many years ago, the possibility of increasing effectiveness has been studied intensively across species since that time (Muchlisin et al 2015;Zhang et al 2015). Although our knowledge and techniques for freezing stallion ejaculate have improved within the last 20 years, a considerable proportion of stallions are still not suitable for semen freezing (Katila 2001).…”

mentioning

confidence: 99%

Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions - A pilot study

Šichtař¹,

Nehasilová²,

Šimoník³

et al. 2017

CZECH J ANIM SCI

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ABSTRACTŠichtař J., Nehasilová A., Šimoník O., Bubeníčková F. (2017): Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions -A pilot study. Czech J. Anim. Sci., 62, 227−233.The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots; these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserved insemination doses (IDs) from each extender (Gent and Lact) were prepared for each stallion from one collection (108 samples from six stallions in total). As a parameter of quality, the motility (computer assisted sperm analysis), viability (fluorescence staining), and morphology (eosin/nigrosine staining) were evaluated after dilution with freezing extenders (fresh) and after thawing (frozen-thawed). The different effects of chosen extenders on the quality of fresh semen were only manifested in higher kinematic parameters of sperm when the Lact extender was used. However, in frozen-thawed samples, the Gent extender yielded significantly better results in all of the evaluated parameters. The representation of sperm subpopulation was significantly influenced by extender in fresh as well as frozen-thawed samples; moreover, we found a significant effect of freezing on the distribution of these subpopulations. The response of individual stallions to chosen extenders was evident in the different quality of fresh as well as frozen-thawed IDs; Gent extender yielded better frozen-thawed IDs. Based on our results, among others describing quality parameters of ejaculate in endangered Old Kladruber stallions, we can recommend using Gent extender for the production of frozen-thawed IDs.

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“…Sperm motility is widely used as a basic marker of semen or spermatozoa quality (Dzyuba et al 2015;Zhang et al 2015;Petelak and Krylov 2016;Simonik et al 2016). Previous studies focusing on the effects of the above-mentioned substances differ in their conclusions as well as in their methodological approaches.…”

Section: Resultsmentioning

confidence: 99%

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

Krejcárková¹,

Folková²,

Šimoník³

et al. 2017

CZECH J ANIM SCI

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Krejcárková A., Folková P., Šimoník O., Šašková M., Krejčířová R., Drábek O., Rajmon R. (2017): Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro. Czech J. Anim. Sci., 62, 435-445.Genistein (GEN) and zearalenone (ZEA), environmental oestrogens commonly present in feedstuff for pigs, are known for their effects on reproductive functions. The aim was to verify the in vitro effects of 0.5-20 µM concentrations of GEN, ZEA and its metabolite α-zearalenol (α-ZOL) on pig sperm motility. A dose-dependent increase of the immotile sperm amount against fast and medium-fast sperm clusters was observed with all three oestrogens from the lowest concentrations tested. Individual CASA (computer-assisted sperm analysis) parameters of motile sperms seemed to be less sensitive indicators. This should be considered especially in toxicological research on a sperm model. Background of inconsistencies in to date-published papers is discussed. The results shift the effective concentrations of ZEA, α-ZOL, and GEN to values achievable in vivo and raises the questions of risk assessment of these compounds in pig reproduction.

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Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Cited by 45 publications

References 26 publications

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions - A pilot study

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

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