Association of hydrogen peroxide with restriction of Septoria tritici in resistant wheat

Shetty, Nandini P.; Kristensen, B.K.; Newman, Mari‐Anne; Möller, Kristian; Gregersen, Per L.; Jørgensen, Hans Jørgen Lyngs

doi:10.1016/s0885-5765(03)00079-1

Cited by 161 publications

(276 citation statements)

References 48 publications

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“…At the five-leaf stage (24Á26 days after sowing), the fourth leaf of each plant was mounted in a horizontal position on bent plastic plates using unbleached cotton strings (Shetty et al 2003). Conidia were harvested in glass-distilled water; the concentration adjusted to 10 6 spores ml (1 and sprayed onto the mounted leaves until run-off, using a glass hand sprayer.…”

Section: Host Pathogen Inoculation and Samplingmentioning

confidence: 99%

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction ofColletotrichum sublineolumin sorghum

Basavaraju

Sekhar

Shetty

et al. 2009

Journal of Plant Interactions

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Hydroxyproline-rich glycoproteins (HRGPs) accumulation and oxidative cross-linking is one of the earliest defense responses in plants against pathogen infection. In the present study HRGP accumulation in three sorghum genotypes i.e. SC146 (resistant), SC326 (intermediately resistant) and BTx623 (susceptible) as a response to Colletotrichum sublineolum isolate CP2126 infection is elucidated. HRGPs were monitored by hydroxyproline (Hyp) estimation. In genotypes SC146 and SC326 there was a significantly higher amounts of Hyp at 2 days after inoculation (dai) compared to genotype BTx623, indicating an infection induced accumulation of HRGPs. Western blot analysis of acid-ethanol extracted proteins with polyclonal antibody raised against pearl millet purified HRGPs identified four bands with molecular masses of Â65, 45, 17 and 14 kDa as HRGPs. Insolubilization of the 45 kDa protein in genotypes SC146 and SC326 upon infection with C. sublineolum indicates a role of this protein in cell wall cross-linking, coinciding with heavier accumulation of hydrogen peroxide. In addition, tissue print analysis using polyclonal antibody of pearl millet HRGPs recognized these cross-linked proteins to be HRGPs. These findings indicated that HRGPs in sorghum is a component of defense reaction against C. sublineolum infection.

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Section: Host Pathogen Inoculation and Samplingmentioning

confidence: 99%

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction ofColletotrichum sublineolumin sorghum

Basavaraju

Sekhar

Shetty

et al. 2009

Journal of Plant Interactions

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“…This fungus causes one of the most economically devastating foliar diseases of wheat, namely, septoria tritici blotch (11). The fungus initially infects the host via hyphal growth through stomatal openings (12,13). Subsequently, it grows slowly into the mesophyll with hyphae extending in the intercellular spaces between the mesophyll cells.…”

mentioning

confidence: 99%

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

Yang

Melo‐Braga

Larsen

et al. 2013

Molecular & Cellular Proteomics

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The fungus Septoria tritici causes the disease septoria tritici blotch in wheat, one of the most economically devastating foliar diseases in this crop. To investigate signaling events and defense responses in the wheat-S. tritici interaction, we performed a time-course study of S. tritici infection in resistant and susceptible wheat using quantitative proteomics and phosphoproteomics, with special emphasis on the initial biotrophic phase of interactions. Our study revealed an accumulation of defense and stress-related proteins, suppression of photosynthesis, and changes in sugar metabolism during compatible and incompatible interactions. However, differential regulation of the phosphorylation status of signaling proteins, transcription and translation regulators, and membraneassociated proteins was observed between two interactions. The proteomic data were correlated with a more rapid or stronger accumulation of signal molecules, including calcium, H 2 O 2 , NO, and sugars, in the resistant than in the susceptible cultivar in response to the infection. Additionally, 31 proteins and 5 phosphoproteins from the pathogen were identified, including metabolic proteins and signaling proteins such as GTP-binding proteins, 14 -3-3 proteins, and calcium-binding proteins. Quantitative PCR analysis showed the expression of fungal signaling genes and genes encoding a superoxide dismutase and cell-wall degrading enzymes. These results indicate roles of signaling, antioxidative stress mechanisms, and nutrient acquisition in facilitating the initial symptomless growth. Taken in its entirety, our dataset suggests interplay between the plant and S. tritici through complex signaling networks and downstream molecular events. Resistance is likely related to several rapidly and intensively triggered signal transduction cascades resulting in a multiple-level activation of transcription and translation processes of defense responses. Our sensitive approaches and model provide a comprehensive (phospho)proteomics resource for studying signaling from the point of view of both host and pathogen during a plant-pathogen interaction. Molecular & Cellular Proteomics 12:

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“…In the wheat-Septoria tritici interaction, infection induced a higher total peroxidase activity and H 2 O 2 levels in resistant than susceptible genotypes (Shetty et al, 2003). These high H 2 O 2 levels restricted further development of hyphae in the incompatible interactions.…”

Section: Discussionmentioning

confidence: 95%

“…These high H 2 O 2 levels restricted further development of hyphae in the incompatible interactions. Apoplastic accumulation of peroxidase transcripts in resistant plants however, did not closely follow H 2 O 2 accumulation, suggesting lack of direct involvement in the production of H 2 O 2 (Shetty et al, 2003). Mlic# ková et al (2004) also detected induced increases in H 2 O 2 levels and total peroxidase activity in resistant Lycopersicon species treated with Oidium neolycopersici.…”

Section: Discussionmentioning

confidence: 98%

“…This is in agreement with results of a recent study of Lan et al (2009) on the involvement of reactive oxygen species (O 2 -and H 2 O 2 ) in the rust resistance response of some sunflower varieties other than those used in this study. In wheat, infected with Septoria tritici, increased peroxidase activity and high levels of H 2 O 2 were associated with reduction in the development of infection structures (Shetty et al, 2003). Hydrogen peroxide can act as an early signal in the induction of cell death and defence gene expression (Neill et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of reactive oxygen species generating enzymes and hydrogen peroxide in the rust resistance response of sunflower (Helianthus annuusL.)

Mohase

Westhuizen

Pretorius

2011

South African Journal of Plant and Soil

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Association of hydrogen peroxide with restriction of Septoria tritici in resistant wheat

Cited by 161 publications

References 48 publications

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction ofColletotrichum sublineolumin sorghum

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction ofColletotrichum sublineolumin sorghum

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

Involvement of reactive oxygen species generating enzymes and hydrogen peroxide in the rust resistance response of sunflower (Helianthus annuusL.)

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