Association of
            <i>Mycoplasma ovipneumoniae</i>
            Infection with Population-Limiting Respiratory Disease in Free-Ranging Rocky Mountain Bighorn Sheep (
            <i>Ovis canadensis canadensis</i>
            )

Besser, Thomas E.; Cassirer, E. Frances; Potter, Kathleen A.; VanderSchalie, John; Fischer, Allison; Knowles, Donald P.; Herndon, David R.; Rurangirwa, Fred R.; Weiser, Glen C.; Srikumaran, Subramaniam

doi:10.1128/jcm.01931-07

Cited by 107 publications

(125 citation statements)

References 26 publications

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“…haemolytica, B. trehalosi, P. multocida, and M. ovipneumoniae have been identified as pathogens associated with pneumonia in BHS (5,9,20,23,27). Of these, M. haemolytica has been shown to consistently induce fatal pneumonia in BHS under experimental conditions (12,19,29).…”

Section: Discussionmentioning

confidence: 99%

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Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

Bavananthasivam

Dassanayake

Kugadas

et al. 2012

Appl Environ Microbiol

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ABSTRACTMannheimia haemolytica,Pasteurella multocida, andBibersteinia trehalosihave been identified in the lungs of pneumonic bighorn sheep (BHS;Ovis canadensis). Of these pathogens,M. haemolyticahas been shown to consistently cause fatal pneumonia in BHS under experimental conditions. However,M. haemolyticahas been isolated by culture less frequently than the other bacteria. We hypothesized that the growth ofM. haemolyticais inhibited by other bacteria in the lungs of BHS. The objective of this study was to determine whetherP. multocidainhibits the growth ofM. haemolytica. Although in monoculture both bacteria exhibited similar growth characteristics, in coculture withP. multocidathere was a clear inhibition of growth ofM. haemolytica. The inhibition was detected at mid-log phase and continued through the stationary phase. When cultured in the same medium, the growth ofM. haemolyticawas inhibited when both bacteria were separated by a membrane that allowed contact (pore size, 8.0 μm) but not when they were separated by a membrane that limited contact (pore size, 0.4 μm). Lytic bacteriophages or bactericidal compounds could not be detected in the culture supernatant fluid from monocultures ofP. multocidaor fromP. multocida-M. haemolyticacocultures. These results indicate thatP. multocidainhibits the growth ofM. haemolyticaby a contact- or proximity-dependent mechanism. If the inhibition of growth ofM. haemolyticabyP. multocidaoccursin vivoas well, it could explain the inconsistent isolation ofM. haemolyticafrom the lungs of pneumonic BHS.

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Section: Discussionmentioning

confidence: 99%

“…Hence, B. trehalosi and P. multocida are unlikely to be the major pathogens of pneumonia in BHS (35). Experimental inoculation studies have shown that M. ovipneumoniae does not cause fatal pneumonia in BHS lambs (5). Nevertheless, it can predispose adult BHS to M. haemolytica pneumonia (6,14).…”

mentioning

confidence: 99%

Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

Bavananthasivam

Dassanayake

Kugadas

et al. 2012

Appl Environ Microbiol

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“…Bacterial pathogens commonly detected in pneumonic bighorn lungs include Mannheimia (Pasteurella) haemolytica, Bibersteinia (Pasteurella) trehalosi, Pasteurella multocida, and Mycoplasma ovipneumoniae (Miller 2001;Besser et al 2008;Wolfe et al 2010). Of these bacteria, M. haemolytica has been most extensively studied as a cause of fatal pneumonia in bighorns (Foreyt et al 1994;Dassanayake et al 2009).…”

Section: Introductionmentioning

confidence: 99%

PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Shanthalingam

Goldy

Bavananthasivam

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:Mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (Ovis canadensis) under experimental conditions. However, Bibersteinia trehalosi and Pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than M. haemolytica by culture-based methods. We hypothesized that assays more sensitive than culture would detect M. haemolytica in pneumonic lung tissues more accurately. Therefore, our first objective was to develop a PCR assay specific for M. haemolytica and use it to determine if this organism was present in the pneumonic lungs of bighorns during the 2009-2010 outbreaks in Montana, Nevada, and Washington, USA. Mannheimia haemolytica was detected by the species-specific PCR assay in 77% of archived pneumonic lung tissues that were negative by culture. Leukotoxin-negative M. haemolytica does not cause fatal pneumonia in bighorns. Therefore, our second objective was to determine if the leukotoxin gene was also present in the lung tissues as a means of determining the leukotoxicity of M. haemolytica that were present in the lungs. The leukotoxin-specific PCR assay detected leukotoxin gene in 91% of lung tissues that were negative for M. haemolytica by culture. Mycoplasma ovipneumoniae, an organism associated with bighorn pneumonia, was detected in 65% of pneumonic bighorn lung tissues by PCR or culture. A PCR assessment of distribution of these pathogens in the nasopharynx of healthy bighorns from populations that did not experience an all-age die-off in the past 20 yr revealed that M. ovipneumoniae was present in 31% of the animals whereas leukotoxin-positive M. haemolytica was present in only 4%. Taken together, these results indicate that culture-based methods are not reliable for detection of M. haemolytica and that leukotoxin-positive M. haemolytica was a predominant etiologic agent of the pneumonia outbreaks of 2009-2010.

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“…The bacterium can be found within the lungs, trachea, and nasal cavity of small ruminants. also Mebratu Asaye et al (2015) isolated many species of bacteria from pneumonic sheep lung but Staphylococcus spp.was the predominant one by percentage of 42.4%on the other hand in this study no detection to P.multocida that is differ from others Rosário Gonçalves (2010) recorded the high mortality rate related to combination of M.ovipneumoniea with P.multocida and Mannheimia haemolytica (the main cause of pneumonia in sheep and goat),which my explained by Besser et al (2008) mentioned that M.ovipneumoniae may act as the only pneumonia pathogen in lamb as it was the predominant one or the primary pathogen which enhances the secondary bacterial infection Post mortem examination, the lesions in the dead animals appear to be the same with Rosário Gonçalves (2010) in epidemiological point of view the deaths were in the summer months (July and August) ( Table 2, Fig 1), the total number of the dead animals are 154 sheep and goats the high percent of deaths occurred in flock 3 in Shrq Aleuaynat by 15% , the high deaths occurred in sheep species by 71.43% while goat species 28.57% the result are agree with Besser et al (2008) who mentioned that M.ovipneumoniae linked to the deaths of bighorn sheep in the Western United States in July 2007.…”

Section: Discussionmentioning

confidence: 66%

Microbiological and Epidemiological Studies on Sheep and Goat Deaths in New Valley Governorates, Egypt

Elnaker¹,

Ibrahim²,

Diab³

2017

AJVS

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Key words:Mycoplasma ovipneumoniae, PCR, pasteurella multocida, deaths in sheep This study was carried out on four flocks of sheep and goats in New Valley governorate in July and August, 2015 in which 154 sheep and goats were dead in these two months after a short course of respiratory signs. It was noticed that the rate of deaths was higher in sheep (71.43%) than in goats (28.57%) and Flock (3) located in Shrq Aleuaynat district showed the highest rate of deaths by 15%. In addition, the highest rate of deaths occurred in age group (8 month) (38.31%) followed by age group (2 month) (25.975%) then the age group (6 month) (24.68%). Postmortem examination (PM) showed that the lesions were restricted to the thoracic cavity where lungs were firm, congested and lobulated with presence of straw fluid in the thoracic cavity with congestion in heart muscles and liver. Bacteriological examination of samples obtained from 13 freshly dead sheep and goats revealed the isolation of 10 isolates of Staphylococcus aureus, 3 isolates of Escherichia coli and 6 isolates of Klebseilla pneumoniae from samples of lung and liver tissues while no isolation was recorded from thoracic fluid, heart tissue or heart blood. PCR technique succeeded in detection of M. ovipneumoniae in two lung samples out of 9 samples at percentage of 22.22%. On contrary, Pasteurella Multocida could not be detected by either traditional culture or PCR technique in the examined samples. Antibiotic sensitivity test revealed that the recovered bacterial isolates were highly sensitive to tulathromycin followed by ciprofloxacin (CIP) while they were less sensitive to tetracycline (TE). Finally, it was concluded that M. Ovipneumoniae may be accused in increased death rates in sheep and goats flocks in New Valley governorate with other bacteria. In addition, it was the first recording of M. Ovipneumoniae in New Valley governorate and further epidemiological studies needed to determine the risk factors associated with occurrence of infection. It was suggested that Tulathromycin should be given to treat respiratory manifestations in sheep.

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Association of Mycoplasma ovipneumoniae Infection with Population-Limiting Respiratory Disease in Free-Ranging Rocky Mountain Bighorn Sheep ( Ovis canadensis canadensis )

Cited by 107 publications

References 26 publications

Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Microbiological and Epidemiological Studies on Sheep and Goat Deaths in New Valley Governorates, Egypt

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