Association of Interleukin-6, Interleukin-12, and Interleukin-10 Gene Polymorphisms with Essential Hypertension in Tatars from Russia

Abstract

Essential hypertension is a common disease with fatal clinical complications. Epidemiological and family studies have confirmed the role of genetic predisposition in its development. Hypertensive patients have been shown to have an altered profile of pro- and anti-inflammatory cytokines. The aim of our investigation was to reveal the association of interleukin-6, interleukin-12, and interleukin-10 gene polymorphisms with essential hypertension and its clinical complications in a Tatar ethnic group from Bashkortostan, Russia. The study involved 362 hypertensive patients and 244 healthy subjects from this Tatar ethnic group (Bashkortostan, Russia). DNA was isolated from whole venous blood using phenol-chloroform extraction by the standard method. IL6 -572 G/C, IL12B 1159 C/A, and IL10 -627 C/A genotypes were typed using polymerase chain reaction followed by restriction enzyme digestion. We found that the IL10 -627 *C/*C genotype was associated with decreased risk of hypertension (OR = 0.64, P = 0.035). IL6 genotypes and allele distribution did not differ significantly between subjects with and without hypertension, but the IL6 -572 *G/*G genotype frequency was found to be significantly higher among those patients who had stroke, compared with normotensive control subjects (P = 0.036). Carriers of the IL12B 1159 *A/*A genotype had a lower risk of stroke (OR = 0.38, P = 0.028). Our study has shown the association between IL10 -627 C/A polymorphism and essential hypertension in the Tatar ethnic group from Bashkortostan, Russia. The IL10 -627*C/*C genotype was found to be protective against hypertension. We also demonstrated that hypertensive patients with the IL12B *A/*A and IL6 *G/*G genotypes had increased risk of stroke. Our results suggest a role for cytokines in cardiovascular disease development in the Tatar ethnic group, but further investigation is needed.

Investigation of relationship between IL-6 gene variants and hypertension in Turkish population

“…In accordance to our findings, no significant differences were found between IL-6 -174 G/C and -572 G/C polymorphisms and HT whereas some other studies showed relations between IL-6 -174 G/C polymorphism and HT and also cardiovascular diseases (CVD) (Humphries et al 2001;Timasheva et al 2008;Losito et al 2003;Pola et al 2002;Wong et al 2007). …”

“…Some studies showed association between -174 G/C or -572 G/C or both polymorphisms and HT whereas some other studies showed no relationship (Wang et al 2011;Humphries et al 2001;Jenny et al 2002;Timasheva et al 2008;Losito et al 2003;Pola et al 2002).…”

“…The IL-6 gene is located at p21 of chromosome 7, contains a 1.3 kb coding sequence with five exons and encodes a 23.7 kd protein. It is a polymorphic gene which -634 C/G, -598 A/G, -597 G/A, -572 G/C, -174 G/C polymorphisms were identified in its promoter region (Wang et al 2011;Nakajima et al 1999;Sesso et al 2006;Gao et al 2011;Jenny et al 2002;Baumann and Gauldie 1994;Wypasek et al 2010;Tanaka et al 2005;Sanders et al 2009;Timasheva et al 2008;Kitamura et al 2002;Illig et al 2004). Therefore most of the studies on IL-6 gene focus on promoter region polymorphism analyses.…”

Analysis of candidate genes of QTL and chromosomal regions for essential hypertension in the rat model

“…Timasheva et al [44] found that carriers of the IL12B 1159 *A/*A genotype have a lower risk of stroke during a study to reveal the association of interleukin-6, interleukin-12, and interleukin-10 gene polymorphisms with essential hypertension, and its clinical complications in a Tatar ethnic group from Bashkortostan, Russia.…”

Sistemas neuromodulatórios: implicações fisiopatológicas

“…Podemos citar a enzima conversora de angiotensina (ECA) (Hilbert et al, 1991), β 2 -adrenérgicos (Li et al, 2007a), interleucina-6, 10 e 12 (Timasheva et al, 2008), ABCA1 e ROS1 (Yamada et al, 2008), aldosterona (White et al, 1999), renina (Mansego et al, 2008), angiotensinogênio (Basarici & Suleymanlar, 2007), NOS (Huang et al, 1995) e muitos outros.…”