Association of SNP g.643G&gt;A of MYF5 gene polymorphism with body weight and body measurements in Bali cattle

Saputra, E. A.; Ulum, Mokhamad Fakhrul; Jakaria, J.

doi:10.14710/jitaa.45.1.1-6

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, some did not. Association studies between MYF5 and PLAG1 with body weight and size in Bali cattle from BPTU-HPT Denpasar have shown no significant (Saputra et al 2020;Fahira et al 2022). In contrast, the previous studies reported that each genotype has a different effect on the economic feature.…”

Section: Association Of Genotype With Growth Traitsmentioning

confidence: 98%

Polymorphism of Melanocortin-4 Receptor Gene and Its Association with Growth Traits in Bali Cattle

Kusminanto,

Hazimi,

Ceri

et al. 2023

JITV

View full text Add to dashboard Cite

The melanocortin-4 receptor (MC4R) is a gene that controls growth traits.Â This gene is embedded in the leptin-melanocortin pathway and regulates body weight.Â Previous studies have successfully identified the genetic diversity of the MC4R associated with growth traits.Â However, studies on Bali cattle are limited.Â This study aimed to identify the SNP markers of the MC4R gene and its effect on birth body weight, weaning body weight, weaning chest girth, weaning withers height, weaning body length, yearling body weight, yearling chest girth, yearling withers height, and yearling body length in Bali cattle (n=43).Â The MC4R genotype was identified by sequencing and aligned using BioEdit v.7.2.5.Â The association between genotype and growth traits was analyzed using the GLM procedure and DMRT in RStudio program v.2022.02. Four SNPs were identified in the exon region: Â g.355GT, g.394CT, g.463GA, and g.682GA. Â Based on the Chi-square test, the population was fitted with HWE (p0.05).Â Genotype-phenotype association based on SNPs showed no significant result (p0.05) where the g.682GA (AG) locus had values of WB (104.33+17.15 kg), WCG (112.83+3.66 kg), WBL (89.50+3.94 kg), YB (124.17+22.44 kg), YCG (120.50+5.50 kg), and YBL (92.83+4.31 kg) higher than the other genotypes.Â Association based on haplotype type showed significant results (p0.05) in BB, where haplotype 2 had the highest BB (21.17+0.75 kg).Â In conclusion, the SNP markers found in this study were polymorphic but did not affect growth traits in Bali cattle.

show abstract

Section: Association Of Genotype With Growth Traitsmentioning

confidence: 98%

Polymorphism of Melanocortin-4 Receptor Gene and Its Association with Growth Traits in Bali Cattle

Kusminanto,

Hazimi,

Ceri

et al. 2023

JITV

View full text Add to dashboard Cite

show abstract

“…In pigs, MYF5 polymorphism was associated with meat quality traits (Liu at al., 2007) and in knockout mice, experiments demonstrated that the MYF5 gene affected muscle development (Lynes et al, 2015). However, the MYF5 gene with SNP g.643G>A had different results in Bali cattle in that it could not be used as a genetic marker for body weight and still needs to be evaluated in a comprehensive study (Saputra et al, 2016).…”

Section: O N L I N E F I R S T a R T I C L Ementioning

confidence: 99%

Profile of Melanocortin-4 Receptor and Myogenic Factor 5 Genes in Pasundan Beef Cattle

Fadholly,

Margawati,

Ibrahim

et al. 2024

PJZ

View full text Add to dashboard Cite

show abstract

“…This enzyme only hit one (1) cutting site: AATT. Saputra et al (2020) stated that restriction enzymes cut DNA fragments at specific base sequences.…”

Section: Polymorphism Of Gh Genementioning

confidence: 99%

Polymorphism of Sikumbang Jonti ducks Growth Hormone (GH) Gene using PCR-RFLP Methods

Rafian¹,

Yurnalis

Husmaini

et al. 2023

Agritropica. J. Agr. Sci.

View full text Add to dashboard Cite

This research aimed to identify polymorphism of the GH gene in Sikumbang Jonti ducks using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method as a candidate for genetic marker selection. This research used 123 blood samples of male and female Sikumbang Jonti ducks. The ducks were eight weeks old and kept intensively at the UPT Fakultas Peternakan Universitas Andalas. Blood samples of Sikumbang Jonti ducks were taken through the brachial vein as much as ± 2 mL. The Genomic DNA Purification Kit (Promega) protocol isolated DNA from blood samples. A pair of primers F amplified the total DNA: 5'-GGA CAG CCT GAG GAA AGA GT-3 'and R: 5'-GTG GAA GGT GGG GAG ACT TC-3', which produced a fragment region of the GH gene exon 3 with a cut region of part intron two and part intron 3 with a primary length of 833 bp. The amplified product was restricted by the enzyme TasI which recognizes the cutting site (↓AATT). Based on the restriction results, two (2) genotypes were obtained, namely homozygous (-/-) as many as four (4) and homozygous (+/+) as many as 119. The analysis of restriction products included the allele frequency, namely the allele (+) of 0.98 and the allele (-) of 0.02, and genotypes frequency of homozygous (-/-) of 0.03 and homozygous genotypes (+/+) of 0.97. The diversity of the GH gene is in the Hardy-Weinberg equilibrium. Based on the research results, it can be concluded that there is diversity in the GH gene of the Sikumbang Jonti duck, and it is in the Hardy-Weinberg equilibrium, so it can be used as a candidate for the genetic marker of ducks.

show abstract

Association of SNP g.643G>A of MYF5 gene polymorphism with body weight and body measurements in Bali cattle

Cited by 3 publications

References 0 publications

Polymorphism of Melanocortin-4 Receptor Gene and Its Association with Growth Traits in Bali Cattle

Polymorphism of Melanocortin-4 Receptor Gene and Its Association with Growth Traits in Bali Cattle

Profile of Melanocortin-4 Receptor and Myogenic Factor 5 Genes in Pasundan Beef Cattle

Polymorphism of Sikumbang Jonti ducks Growth Hormone (GH) Gene using PCR-RFLP Methods

Contact Info

Product

Resources

About