Association of the CETP TaqIB Polymorphism with Coronary Artery Disease in Type 2 Diabetic Patients

Abstract

Background and Objective: Diabetes mellitus is the most common risk factor for coronary artery disease (CAD). Cholesteryl ester transfer protein (CETP) TaqIB polymorphism is associated with changes in lipid profile and may be a risk factor for CAD in patients with diabetes. This study aimed to evaluate the association of CETP TaqIB polymorphism with CAD in patients with type 2 diabetes.Methods: In this case-control study, 292 diabetic patients were divided into two groups based on angiography reports (150 participants with normal angiogram as the control group and 142 participants with more than 50% stenosis of at least one coronary artery as the case group). The CETP TaqIB genotypes were determined by PCR-RFLP analysis. Fasting blood glucose was measured using glucose oxidase and lipid profile (triglycerides, total cholesterol, high density lipoprotein-cholesterol and low density lipoprotein-cholesterol) by an enzymatic method.Results: There was no significant difference in the frequency of genotypes and alleles between case and control group (control group: B1B1, 17.3%; B1B2, 63.3%; and B2B2, 19.3%; case group: B1B1, 18.3%; B1B2, 64.1%; and B2B2, 17.6%) (P=0.92). In the control group, subjects with heterozygous genotype (B1B2 genotype) had higher levels of cholesterol compared with the other genotypes (B1B1 and B2B2 genotypes). Also, in the case group, subjects with B1B2 genotype had significantly higher weight (P=0.013).Conclusion: There is no significant correlation between CETP TaqIB polymorphism and the increased risk of coronary artery disease in patients with type 2 diabetes.

Interaction between CETP Taq1B polymorphism and HEI, DQI and DPI on metabolic biomarkers in patients with type 2 diabetes

“…Parallel with our result, other studies have reported an insignificant association between lipid profile and CETP Taq1B polymorphism. [56][57][58] By contrast to our results, some studies showed a significant association of CETP Taq1B polymorphism with HDL-C concentrations. [59][60][61] This inconsistency might relate to some factors such as sample size, ethnic group and underlying disease.…”

“…According to our previous study, 25 although the HDL‐C concentrations in B2B2 patients were higher than for the B1B1 genotype, the relationship between the genotypes and HDL‐C levels was not significant. Parallel with our result, other studies have reported an insignificant association between lipid profile and CETP Taq1B polymorphism 56–58 . By contrast to our results, some studies showed a significant association of CETP Taq1B polymorphism with HDL‐C concentrations 59–61 .…”

“…Besides, CETP Taq1B is not the only polymorphism affecting the HDL-C concentrations and it may depend on other polymorphisms in the promotor region of the gene. 56,[62][63][64] Our data indicated an inverse association between DQI and HDL-C concentrations. The mean of HDL-C concentrations deccreased across the DQI tertiles.…”

“…As we mentioned before, the results on the association between CETP Taq1B polymorphism and blood lipid profile are inconsistent. 56,59 However, in our previous study, we could show that diet has a notable effect on the relationship between the genotypes and lipid profile, particularly HDL-C concentrations, among T2DM patients and we postulated that B1B1 individuals with an isocaloric high-fat diet might have higher HDL-C concentrations. 25 Li et al 24 also reported a significant interaction among diabetic men.…”

“…This inconsistency might relate to some factors such as sample size, ethnic group and underlying disease. Besides, CETP Taq1B is not the only polymorphism affecting the HDL‐C concentrations and it may depend on other polymorphisms in the promotor region of the gene 56,62–64 …”