Astrocytic and neuronal biochemical markers in the sera of subjects with diabetes mellitus

Hovsepyan, Meri; Haas, Michael J.; Boyajyan, Anna; Guevorkyan, A. A.; Mamikonyan, A. A.; Myers, Susan E.; Mooradian, Arshag D.

doi:10.1016/j.neulet.2004.07.071

Cited by 35 publications

(28 citation statements)

References 17 publications

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“…This indicated that diabetic patients showed some sort of neuronal damage and/or bloodbrain barrier disruption. This finding was in agreement with Hovsepyan et al (16) who demonstrated a significant increase in antibodies against NSE in both type 1 and type 2 diabetic subjects compared to healthy controls. In hypertensive patients and patients with ischemic heart diseases, the mean serum NSE ranked an intermediate values between ischemic stroke and healthy controls.…”

Section: Discussionsupporting

confidence: 93%

Salivary neuron specific enolase: an indicator for neuronal damage in patients with ischemic stroke and stroke-prone patients

Al‐Rawi

Atiyah

2009

Clinical Chemistry and Laboratory Medicine

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Background: The blood-brain barrier is compromised in patients with stroke. The release of neuro-biochemical protein markers, such as neuron specific enolase (NSE) into the circulation may allow the pathophysiology and prognosis of patients with cerebrovascular diseases to be evaluated further. The present study was designed to measure the marker of neuronal damage, NSE, in saliva and serum of patients with acute ischemic stroke and patients with stroke related diseases as a diagnostic and/or monitoring tool for early prediction of ischemic stroke. Methods: Salivary and serum NSE concentrations were measured in 150 individuals. Fifty were patients recently diagnosed as having ischemic stroke, 75 were gender and age-matched risk-group patients (patients with hypertension, type 2 diabetes and ischemic heart disease). Another 25 were gender and age-matched healthy controls. Results: Salivary and serum NSE concentrations were significantly higher than that of healthy controls. The

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Section: Discussionsupporting

confidence: 93%

Salivary neuron specific enolase: an indicator for neuronal damage in patients with ischemic stroke and stroke-prone patients

Al‐Rawi

Atiyah

2009

Clinical Chemistry and Laboratory Medicine

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“…Glycolysis processes in a β-cell are disordered (and hence, insulin production is reduced) without NSE or if its concentration is low, which can eventually lead to cell death. This hypothesis is confi rmed by the data on a signifi cant increase in the level of autoantibodies to NSE in DM1 patients [7].…”

Section: Resultsmentioning

confidence: 72%

Immunoreactivity of Neuron-Specific Enolase (NSE) in Human Pancreas in Health and Type 1

et al. 2010

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The role of neuron-specific enolase (glycolytic enzyme; marker of nerve fibers and Langerhans islet in human pancreas) in the development of type 1 diabetes mellitus was studied in autopsy specimens from 6 adult patients. Autopsied specimens of the pancreas from 7 subjects without carbohydrate metabolism disorders served as the control. Autopsied specimens of the pancreas from a child with the clinical diagnosis of type 1 diabetes mellitus, a child without carbohydrate metabolism disorders, and from 7 human fetuses of 15-40 weeks gestation were also studied. In control specimens, the neuron-specific enolase was detected in the pancreatic nerve fibers and Langerhans islets. Studies of pancreatic tissue specimens from adult patients with type 1 diabetes mellitus showed no immunopositive reaction to neuron-specific enolase in insulin-negative specimens. A possible mechanism of type 1 diabetes mellitus development is suggested.

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“…However, several other lines of evidence support our findings. It has been shown that serum S100β concentrations in type 2 diabetic, but not type 1 diabetic humans are significantly lower than those found in healthy controls (Hovsepyan et al, 2004). In addition, experiments using cultured astrocytes have shown that chronic elevated glucose and beta-hydroxy-butyrate levels affect astrocyte activity by reducing extracellular secretion of S100β (Leite et al, 2004; Nardin et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

High-fat diet and age-dependent effects on enteric glial cell populations of mouse small intestine

Stenkamp-Strahm

Patterson

Boren

et al. 2013

Autonomic Neuroscience

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Diabetes and obesity are increasing in prevalence at an alarming rate throughout the world. Autonomic diabetic neuropathy is evident in individuals that experience a long-standing diabetic disease state, and gastrointestinal (GI) dysmotility is thought to be the outcome of neuropathies within the enteric nervous system (ENS) of these patients. To date, an analysis of enteric glial cell population changes during diabetic symptoms has not been performed, and may bring insight into disease pathology and neuropathy, given glial cell implications in gastrointestinal and neuronal homeostasis. Diabetes and obesity were monitored in C57Bl/6J mice fed a 72% high-fat diet, and duodenal glial expression patterns were evaluated by immunohistochemistry and RT-PCR for S100β, Sox10 and GFAP proteins and transcripts, as well as transmission electron microscopy (TEM). The high-fat diet caused obesity, hyperglycemia and insulin resistance after 4 weeks. These changes were associated with a significant decline in the area density indices of mucosa-associated glial cell networks, evidenced by S100β staining at 8 and 20 weeks. All three markers and TEM showed that myenteric glial cells were unaffected by early and late disease periods. However, analysis of Sox10 transcript expression and immunoreactivity showed a diet independent, age-associated decline in glial cell populations. This is the first study showing that mucosal glia cell damage occurs during diabetic symptoms, suggesting that mucosal enteric glia injury may have a pathophysiological significance during this disease. Our results also provide support for age-associated changes in longitudinal studies of enteric glial cells.

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Astrocytic and neuronal biochemical markers in the sera of subjects with diabetes mellitus

Cited by 35 publications

References 17 publications

Salivary neuron specific enolase: an indicator for neuronal damage in patients with ischemic stroke and stroke-prone patients

Salivary neuron specific enolase: an indicator for neuronal damage in patients with ischemic stroke and stroke-prone patients

Immunoreactivity of Neuron-Specific Enolase (NSE) in Human Pancreas in Health and Type 1

High-fat diet and age-dependent effects on enteric glial cell populations of mouse small intestine

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