Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis

Menssen, Ruth; Neutzner, Albert; Seufert, Wolfgang

doi:10.1016/s0960-9822(01)00095-1

Cited by 83 publications

(94 citation statements)

References 24 publications

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“…Despite Cdc15 protein being present at constant levels throughout the cell cycle , we and others found that Cdc15 only localized to SPBs during anaphase and telophase (Cenamor et al, 1999;Gruneberg et al, 2000;Xu et al, 2000;Menssen et al, 2001). However, there are slight variations in the localization pattern observed for Cdc15, which appears to depend on the epitope tag used in the different studies.…”

Section: The Localization Of Cdc15mentioning

confidence: 49%

“…Several reports had shown that Cdc15 localizes to SPBs (Cenamor et al, 1999;Xu et al, 2000;Gruneberg et al, 2000;Menssen et al, 2001). However, slight differences in the dynamics of Cdc15 localization had been observed, depending on the epitope tag used.…”

Section: Cdc15 Localizes To Both Spindle Pole Bodies During Anaphasementioning

confidence: 99%

“…Cdc15 protein levels and associated kinase activity are constant throughout the cell cycle ; however, Cdc15 localization and its phosphorylation status change during the cell cycle. Cdc15 associates with the cytoplasmic face of SPBs during anaphase and is dephosphorylated during exit from mitosis (Cenamor et al, 1999;Gruneberg et al, 2000;Jaspersen and Morgan, 2000;Xu et al, 2000;Menssen et al, 2001). The protein levels of the related protein kinases Dbf2 and Dbf20 are also constant throughout the cell cycle , but Dbf2 kinase activity fluctuates during the cell cycle, being low during G1, S phase, and early mitosis, and high during anaphase and exit from mitosis .…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the Mitotic Exit Protein Kinases Cdc15 and Dbf2

Visintin

Amon

2001

MBoC

131

183

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In budding yeast, the release of the protein phosphatase Cdc14 from its inhibitor Cfi1/Net1 in the nucleolus during anaphase triggers the inactivation of Clb CDKs that leads to exit from mitosis. The mitotic exit pathway controls the association between Cdc14 and Cfi1/Net1. It is comprised of the RAS-like GTP binding protein Tem1, the exchange factor Lte1, the GTPase activating protein complex Bub2-Bfa1/Byr4, and several protein kinases including Cdc15 and Dbf2. Here we investigate the regulation of the protein kinases Dbf2 and Cdc15. We find that Cdc15 is recruited to both spindle pole bodies (SPBs) during anaphase. This recruitment depends on TEM1 but not DBF2 or CDC14 and is inhibited by BUB2. Dbf2 also localizes to SPBs during anaphase, which coincides with activation of Dbf2 kinase activity. Both events depend on the mitotic exit pathway components TEM1 and CDC15. In cells lacking BUB2, Dbf2 localized to SPBs in cell cycle stages other than anaphase and telophase and Dbf2 kinase was prematurely active during metaphase. Our results suggest an order of function of mitotic exit pathway components with respect to SPB localization of Cdc15 and Dbf2 and activation of Dbf2 kinase. BUB2 negatively regulates all 3 events. Loading of Cdc15 on SPBs depends on TEM1, whereas loading of Dbf2 on SPBs and activation of Dbf2 kinase depend on TEM1 and CDC15. INTRODUCTIONThe final stage of the cell cycle is exit from mitosis (reviewed in Morgan, 1999;Zachariae, 1999). During this time, the mitotic spindle disassembles, chromosomes decondense, and cytokinesis occurs. In the budding yeast Saccharomyces cerevisiae, these events are brought about by inactivation of Clb cyclin-dependent kinases (Clb-CDKs), which, earlier in the cell cycle, trigger entry into mitosis (reviewed in Zachariae and Nasmyth, 1999). Inactivation of Clb-CDKs is brought about by 2 redundant mechanisms. Ubiquitin-dependent degradation of Clb cyclins by the anaphase promoting complex (APC) or cyclosome (C) complexed with the APC activator Cdh1 (APC/C Cdh1 ) and binding of the CDK inhibitor Sic1 to the Clb-CDK complex (Schwab et al., 1997;Visintin et al., 1997;Fang et al., 1998;Shirayama et al., 1998; Kotani et al.1999;Kramer et al., 2000;Yudkovsky et al., 2000). The protein phosphatase Cdc14 plays an important role in both stimulating APC-dependent degradation of mitotic Clb cyclins and accumulation of Sic1 (Visintin et al., 1998;Jaspersen et al., 1999). Cdc14 dephosphorylates the APC-specificity factor Cdh1, the CDK inhibitor Sic1 and its transcription factor Swi5, allowing for activation of Cdh1 and transcription and stabilization of Sic1, respectively. Cdc14 is regulated by Cfi1/Net1 Straight et al., 1999;Visintin et al., 1999;Traverso et al., 2001). Cfi1/ Net1 inhibits Cdc14 in the nucleolus during G1, S phase, and early mitosis. During nuclear division, Cdc14 is released from Cfi1/Net1, allowing Cdc14 to reach its targets. The mitotic exit pathway is required for the release of Cdc14 from Cfi1/Net1 Visintin et al., 1999). This pathway includes the Ras-l...

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Section: The Localization Of Cdc15mentioning

confidence: 49%

Section: Cdc15 Localizes To Both Spindle Pole Bodies During Anaphasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of the Mitotic Exit Protein Kinases Cdc15 and Dbf2

Visintin

Amon

2001

MBoC

131

183

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“…Besides their roles in mitotic exit, components in the MEN such as Cdc5, Tem1, Cdc15, Dbf2-Mob1, and Cdc14 are required for proper actin ring formation at the mother-bud-neck (Jimenez et al, 1998;Frenz et al, 2000;Lee et al, 2001). Consistent with their roles in cytokinesis, both Cdc15 and Dbf2-Mob1 localize to the bud-neck in late mitosis following the downregulation of Cdk1 activity (Xu et al, 2000;Luca et al, 2001;Menssen et al, 2001;Bardin et al, 2003;Lim et al, 2003). Unlike Cdc15 and Dbf2, however, Cdc5 localizes to the neck as early as G2 (prior to Cdc28 downregulation) and remains at this structure until late mitosis , suggestive of additional role(s) at the neck.…”

Section: Cytokinesismentioning

confidence: 97%

Yeast polo-like kinases: functionally conserved multitask mitotic regulators

et al. 2005

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The polo-like kinases (Plks) are a conserved subfamily of Ser/Thr protein kinases that play pivotal roles in regulating various cellular and biochemical events at multiple stages of M phase. Genetic and biochemical data revealed that both the budding yeast and the fission yeast polo kinase homologs (Cdc5 and Plo1, respectively) bear remarkable functional similarities with those in metazoan organisms, suggesting that the role of Plks is largely conserved throughout evolution. Thus, studies on Plks in genetically amenable lower eucaryotic organisms may yield valuable insights into the function of Plks in higher eucaryotic organisms. In this review, common properties and distinct functions of Cdc5 and Plo1 will be discussed and compared to properties and functions of Plks in higher eucaryotic organisms.

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“…Nevertheless, recent evidence suggests that MEN signalling components have more direct roles in orchestrating the process of cytokinesis. On one hand, Dbf2p and Mob1p have been found at the actin-myosin ring (AMR) structure that is essential for abscission [35][36][37][38], and Cdc15p, Dbf2p and Mob1p are required for AMR contraction [39,40]. Moreover, current evidence suggests that the MEN components Dbf2p and Mob1p can regulate the cytokinetic components Chs2p, Hof1p, and Inn1p (summarised in [33,41]).…”

Section: Mitotic Exit Network (Men) In S Cerevisiaementioning

confidence: 99%

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

Hergovich

Hemmings

2012

Seminars in Cell & Developmental Biology

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Over the past decade Hippo kinase signalling has been established as an essential tumour suppressor pathway controlling tissue growth in flies and mammals. All members of the Hippo core signalling cassette are conserved from yeast to humans, whereby the yeast analogues of Hippo, Mats and Lats are central components of the mitotic exit network and septation initiation network in budding and fission yeast, respectively. Here, we discuss how far core Hippo signalling components in Drosophila melanogaster and mammals have reported similar mitotic functions as already established for their highly conserved yeast counterparts.

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Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis

Cited by 83 publications

References 24 publications

Regulation of the Mitotic Exit Protein Kinases Cdc15 and Dbf2

Regulation of the Mitotic Exit Protein Kinases Cdc15 and Dbf2

Yeast polo-like kinases: functionally conserved multitask mitotic regulators

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

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