Asymmetrical 3D Nanoceria Channel for Severe Neurological Defect Regeneration

Qian, Yun; Han, Qixin; Zhao, Xiaotian; Li, Hui; Yuan, Weien; Fan, Cunyi

doi:10.1016/j.isci.2019.01.013

Cited by 42 publications

(36 citation statements)

References 51 publications

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“…Melatonin‐loaded polycaprolactone scaffolds alleviated immune insult and improved repair of a 15 mm long sciatic nerve insult by regulating the autophagy signalling pathway . Three‐dimensional nanoceria‐based nerve conduit and nanodiamond channels also contributed to restoration of immune homeostasis by reducing regional oxidative stress and inflammatory reactions in lengthy sciatic nerve defects . Inflammation and oxidative stress are also responsible for radiation‐induced peripheral neuropathy.…”

Section: Discussionmentioning

confidence: 99%

(‐)‐Epigallocatechin gallate‐loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis

et al. 2019

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Objectives In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (‐)‐Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages. Materials and methods An EGCG‐loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state. Results RSCs and RSMCs exhibited higher proliferative, anti‐oxidant and anti‐inflammatory states in an EGCG/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators. Conclusions The EGCG‐modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.

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Section: Discussionmentioning

confidence: 99%

(‐)‐Epigallocatechin gallate‐loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis

et al. 2019

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“…They showed that nanoceria, as a strong self-regenerative antioxidant, can behave as a powerful ROS scavenger to inhibit cell senescence and stimulate neurite sprouting. In 2019, Qian et al evaluated the therapeutic effects of nanoceria in a 3D composite channel for treating a severe neurological defect [ 127 ]. To this end, they incorporated 0.5%, 1%, and 2%–4% nanoceria into a collagen/PCL (COL/PCL) blend to prepare composite conduits by asymmetrical 3D manufacturing.…”

Section: Nanoceria For Soft-tissue Engineeringmentioning

confidence: 99%

“…( B ) Microscopic images exhibiting the triple immunofluorescence of regenerated nerves indicating axonal restoration after 18 weeks of the implantation: A, E, I, and M were stained by DAPI; B, F, J, and N were stained by NF200; C, G, K, and O were stained by Tuj1; and D, H, L, and P show merged images. Reproduced with permission from [ 127 ].…”

Section: Figurementioning

confidence: 99%

Cerium Oxide Nanoparticles (Nanoceria): Hopes in Soft Tissue Engineering

et al. 2020

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Several biocompatible materials have been applied for managing soft tissue lesions; cerium oxide nanoparticles (CNPs, or nanoceria) are among the most promising candidates due to their outstanding properties, including antioxidant, anti-inflammatory, antibacterial, and angiogenic activities. Much attention should be paid to the physical properties of nanoceria, since most of its biological characteristics are directly determined by some of these relevant parameters, including the particle size and shape. Nanoceria, either in bare or functionalized forms, showed the excellent capability of accelerating the healing process of both acute and chronic wounds. The skin, heart, nervous system, and ophthalmic tissues are the main targets of nanoceria-based therapies, and the other soft tissues may also be evaluated in upcoming experimental studies. For the repair and regeneration of soft tissue damage and defects, nanoceria-incorporated film, hydrogel, and nanofibrous scaffolds have been proven to be highly suitable replacements with satisfactory outcomes. Still, some concerns have remained regarding the long-term effects of nanoceria administration for human tissues and organs, such as its clearance from the vital organs. Moreover, looking at the future, it seems necessary to design and develop three-dimensional (3D) printed scaffolds containing nanoceria for possible use in the concepts of personalized medicine.

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“…Fused deposition modeling (FDM) is a printing technology. A temperature-sensitive polymer is heated to a molten state and then deposited on a solid medium through a printing nozzle (Qian et al, 2019). The advantage of this kind of technology is its high precision.…”

Section: Extrusion-based Printingmentioning

confidence: 99%

Additive Manufacturing of Nerve Guidance Conduits for Regeneration of Injured Peripheral Nerves

Song

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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As a common and frequent clinical disease, peripheral nerve defect has caused a serious social burden, which is characterized by poor curative effect, long course of treatment and high cost. Nerve autografting is first-line treatment of peripheral nerve injuries (PNIs) but can result in loss of function of the donor site, neuroma formation, and prolonged operative time. Nerve guidance conduit (NGC) serves as the most promising alternative to autologous transplantation, but its production process is complicated and it is difficult to effectively combine growth factors and bioactive substances. In recent years, additive manufacturing of NGCs has effectively solved the above problems due to its simple and efficient manufacturing method, and it can be used as the carrier of bioactive substances. This review examines recent advances in additive manufacture of NGCs for PNIs as well as insight into how these approaches could be improved in future studies.

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Asymmetrical 3D Nanoceria Channel for Severe Neurological Defect Regeneration

Cited by 42 publications

References 51 publications

(‐)‐Epigallocatechin gallate‐loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis

(‐)‐Epigallocatechin gallate‐loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis

Cerium Oxide Nanoparticles (Nanoceria): Hopes in Soft Tissue Engineering

Additive Manufacturing of Nerve Guidance Conduits for Regeneration of Injured Peripheral Nerves

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