Atomic force microscopy-based bioanalysis for the study of disease

Morton, Kirstin C.; Baker, Lane A.

doi:10.1039/c4ay00485j

Cited by 18 publications

(29 citation statements)

References 315 publications

(360 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4,[11][12][13][14] Both techniques have also been combined. 9,15,16 The imaging mechanism in AFM is based on mechanical forces between the sample and the tip of a cantilever, which is used to track the surface of the sample (Fig. 1a).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

et al. 2015

View full text Add to dashboard Cite

Atomic force microscopy (AFM) and scanning ion conductance microscopy (SICM) are excellent and commonly used techniques for imaging the topography of living cells with high resolution. We present a direct comparison of AFM and SICM for imaging microvilli, which are small features on the surface of living cells, and for imaging the shape of whole cells. The imaging quality on microvilli increased significantly after cell fixation for AFM, whereas for SICM it remained constant. The apparent shape of whole cells in the case of AFM depended on the imaging force, which deformed the cell. In the case of SICM, cell deformations were avoided, owing to the contact-free imaging mechanism. We estimated that the lateral resolution on living cells is limited by the cell's elastic modulus for AFM, while it is not for SICM. By long-term, time-lapse imaging of microvilli dynamics, we showed that the imaging quality decreased with time for AFM, while it remained constant for SICM.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

et al. 2015

View full text Add to dashboard Cite

show abstract

“…thioflavin T, or ThT, fluorescence assay, CD spectroscopy, IR spectroscopy, and NMR spectroscopy) 4,5 , and then visualized by single molecule techniques, such as transmittance electron microscopy (TEM) and atomic force microscopy (AFM). 6–9 For both popular tools, samples are typically dried at various aggregation stages for further characterization of the amyloid species prepared in solution. Among different types of aggregates, structured oligomers have been recognized as potentially more toxic species than other structures.…”

Section: Introductionmentioning

confidence: 99%

Potential Artifacts in Sample Preparation Methods Used for Imaging Amyloid Oligomers and Protofibrils due to Surface-Mediated Fibril Formation

et al. 2017

View full text Add to dashboard Cite

Accurate imaging of nanometer-sized structures and morphologies is essential to characterizing amyloid species formed at various stages of amyloid aggregation. In this report, we examine the effect of different drying procedures on the final morphology of surface-mediated fibrils formed during the incubation period, which may be then mistaken as oligomers or protofibrils intentionally formed in solution for a particular study. AFM results show that some artifacts, such as globules, flake-like structures, and even micrometer-long fibrils, can be produced under various drying conditions. We also demonstrate that one can prevent drying artifacts by using an appropriate spin-coating procedure to dry amyloid samples. This procedure can bypass the wetting/dewetting transition of the liquid layer during the drying process, and preserve the structure of interest on the substrate without generating drying artifacts.

show abstract

“…The method allows the direct imaging of nanoscale features both on cell surface and along the length of a single microvillus. This method is compatible with both commercially available standalone AFM tools and those used in conjunction with optical microscopy techniques 28 , which makes it amenable to field deployed screening of diseases 29 related to cell malfunction. We anticipate that this microscopy based methodology will have an impact in the emerging field of digital pathology 30 , where it is important to register information on diseased cells in a fixed state that is free of ambient contaminants and of sufficiently high-spatial resolution for algorithm based data analysis.…”

Section: Introductionmentioning

confidence: 99%

Subcellular Imaging of Liquid Silicone Coated-Intestinal Epithelial Cells

Nirmalraj

Lehner

Thompson

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Surface contamination and the formation of water bridge at the nanoscopic contact between an atomic force microscope tip and cell surface limits the maximum achievable spatial resolution on cells under ambient conditions. Structural information from fixed intestinal epithelial cell membrane is enhanced by fabricating a silicone liquid membrane that prevents ambient contaminants and accumulation of water at the interface between the cell membrane and the tip of an atomic force microscope. The clean and stable experimental platform permits the visualisation of the structure and orientation of microvilli present at the apical cell membrane under standard laboratory conditions together with registering topographical features within a microvillus. The method developed here can be implemented for preserving and imaging contaminant-free morphology of fixed cells which is central for both fundamental studies in cell biology and in the emerging field of digital pathology.

show abstract

Atomic force microscopy-based bioanalysis for the study of disease

Abstract: This review aims to describe the operation of atomic force microscopy related to the study of disease states and single cell analysis, and to serve as an overview of recent advances in this subject area.

Cited by 18 publications

References 315 publications

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

Potential Artifacts in Sample Preparation Methods Used for Imaging Amyloid Oligomers and Protofibrils due to Surface-Mediated Fibril Formation

Subcellular Imaging of Liquid Silicone Coated-Intestinal Epithelial Cells

Contact Info

Product

Resources

About