Atomic Force Microscopy-Based Molecular Recognition of a Fibrinogen Receptor on Human Erythrocytes

Carvalho, Filomena A.; Connell, Simon D.; Miltenberger-Miltényi, Gábriel; Pereira, Sonia; Tavares, Anna Carolina Faria Moreira Gomes; Ariëns, Robert A. S.; Santos, Nuno C.

doi:10.1021/nn1009648

Cited by 139 publications

(150 citation statements)

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“…The initiation of cell adhesion www.ecmjournal.org E Lamers et al Dynamic cell adhesion and migration on nanogrooves is a process which is usually established within a few microseconds (Carvalho et al, 2010) and involves integrins that adhere to surface specifi c proteins such as fi bronectin and vitronectin (Pierres et al, 2008;Siebers et al, 2005). Within 5 min, these integrins cluster to become focal adhesions (FAs), and may either disassemble or further mature into fi brillar adhesions (FBs) within 20 min (Gardel et al, 2010;Zaidel-Bar et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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Organised nanotopography mimicking the natural extracellular matrix can be used to control morphology, cell motility, and differentiation. However, it is still unknown how specifi c cell types react with specifi c patterns. Both initial adhesion and preferential cell migration may be important to initiate and increase cell locomotion and coverage with cells, and thus achieve an enhanced wound healing response around an implantable material. Therefore, the aim of this study was to evaluate how MC3T3-E1 osteoblast initial adhesion and directional migration are infl uenced by nanogrooves with pitches ranging from 150 nm up to 1000 nm. In this study, we used a multipatterned substrate with fi ve different groove patterns and a smooth area with either a concentric or radial orientation. Initial cell adhesion measurements after 10 s were performed using atomic force spectroscopy-assisted single-cell force spectroscopy, and demonstrated that nascent cell adhesion was highly induced by a 600 nm pitch and reduced by a 150 nm pitch. Addition of RGD peptide signifi cantly reduced adhesion, indicating that integrins and cell adhesive proteins (e.g. fi bronectin or vitronectin) are key factors in specifi c cell adhesion on nanogrooved substrates. Also, cell migration was highly dependent on the groove pitch; the highest directional migration parallel to the grooves was observed on a 600 nm pitch, whereas a 150 nm pitch restrained directional cell migration. From this study, we conclude that grooves with a pitch of 600 nm may be favourable to enhance fast wound closure, thereby promoting tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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“…This phenomenon can be explained by Bell-Evans model [55], which characterizes the behavior of molecular unbinding pulled by an external force. SMFS is a mature single-molecule technique and many researchers have used SMFS to measure the molecular binding force on cells grown in vitro, such as receptor-drug [41], receptor-ligand [56], fibrinogen-erythrocyte [57], and aptamer-protein [58], showing that the binding force of receptor-ligand was in the range of 20-200 pN [59]. Here, we measured the CD20-rituximab binding force on patient cancer cells based on the fluorescence recognition of the specific cancer cell surface marker and the binding force was in the range of molecular binding force.…”

Section: Force Spectroscopy Of Molecular Interactions On Tumor Cells mentioning

confidence: 99%

Investigating the Molecular Specific Interactions on Cell Surface Using Atomic Force Microscopy

Liu

et al. 2015

Micro‐ and Nanomanipulation Tools

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Cancer is the leading cause of death in economically developed countries and the second leading cause of death in developing countries [1]. The new cancer cases will increase from 12.7 million in 2008 to 22.2 million by 2030, while the cancer-related deaths will increase from 7.6 million in 2008 to 13.2 million by 2030 [2]. Carcinogenesis and tumor progression are complex and progressive processes that are associated with numerous genetic and epigenetic alterations [3]. Our growing understanding of tumor biology and genomics paves the way to the development of new therapy approaches, and marked progress has been achieved in overcoming treatment resistance through precision medicine and immunotherapy [4]. However, because of the fact that the exact reason why a cell becomes cancerous is unknown (the only one cancer whose cause is clear is cervical cancer), the current cancer treatments cannot prevent the recurrence of cancers and the age-adjusted mortality rate for cancer is about the same in the twenty-first century as it was 50 years ago [5].Lymphomas, solid tumors of the immune system [6], account for 4%-5% of all cancers [7]. Hodgkin's lymphoma accounts for about 10% of all lymphomas and the remaining 90% are referred to as non-Hodgkin lymphoma (NHL) [6]. NHL can be divided into many subtypes according to the combination of morphology, immunophenotype, genetic, molecular, and clinical features of the tumors [8]. Approximately, 85% of NHL in adults arises from B cells [9], and the rest are T-cell origin [10]. Follicular lymphoma and diffuse large B-cell lymphoma are the two most common B-cell NHLs, comprising 60% of new B-cell NHL diagnoses each year in North America [11]. In 1997, US Food and Drug Administration (FDA) approved rituximab (an anti-CD20 monoclonal antibody (mAb)) for treating B-cell NHLs. CD20 is 297 amino acids long with a molecule weight of about 33 kDa [12]. The exact biological function of CD20 is currently unknown [13], partly because it has no known natural ligand and CD20 knockout mice display an almost normal phenotype [14]. Many of the functions of CD20 have been determined using artificial ligands (antibody) [15]. In vitro experiments proposed that CD20

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“…Furthermore, purified proteins may not fully represent proteins in cell membranes. Hence, SMFS on cells [36,[43][44][45][46][47] that are attached to a substrate (e.g., glass slide, Petri dish) can detect molecular interactions in a native environment. In 2006, Puntheeranurak et al [43] investigated molecular forces on SGLT1- [47] investigated the specific molecular interactions between the extracellular matrix protein Tenascin-C and the aptamer GBI-10 on live U251 cells, and measured the binding force and the dissociation constant for the GBI-10/Tenascin-C complex.…”

Section: Measuring the Affinity And Distribution Of Membrane Proteinsmentioning

confidence: 99%

Progress in measuring biophysical properties of membrane proteins with AFM single-molecule force spectroscopy

Liu

et al. 2014

Chin. Sci. Bull.

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Membrane proteins are crucial in cell physiological activities and are the targets for most drugs. Thus, investigating the behaviors of membrane proteins not only provide deeper insights into cell function, but also help disease treatment and drug development. Atomic force microscopy is a unique tool for investigating the structure of membrane proteins. It can both image the morphology of single native membrane proteins with high resolution and, via single-molecule force spectroscopy (SMFS), directly measure their biophysical properties during molecular physiological activities such as ligand binding and protein unfolding. In the context of molecular biomechanics, SMFS has been successfully used to understand the structure and function of membrane proteins, complementing the static three-dimensional structures of proteins obtained by X-ray crystallography. Here, based on the authors' antigen-antibody binding force measurements in clinical tumor cells, the principle and method of SMFS is discussed, the progress in using SMFS to characterize membrane proteins is summarized, and challenges for SMFS are presented.

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Atomic Force Microscopy-Based Molecular Recognition of a Fibrinogen Receptor on Human Erythrocytes

Cited by 139 publications

References 42 publications

Dynamic cell adhesion and migration on nanoscale grooved substrates

Dynamic cell adhesion and migration on nanoscale grooved substrates

Investigating the Molecular Specific Interactions on Cell Surface Using Atomic Force Microscopy

Progress in measuring biophysical properties of membrane proteins with AFM single-molecule force spectroscopy

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