“…First, force/distance curves were obtained after prolonged incubation at 37 8C. Then, the adhesion force was monitored at 3,5,10,15,20,30,60,120,180, and 240 min immediately after the temperature in the AFM fluid cuvette decreased to 20 8C. It was noteworthy that water in the liquid cuvette attained the pre-set temperature within 2 min (see Figure S4 in the Supporting Information).…”