Atorvastatin inhibits brain oxidative stress of Streptozotocininduced diabetic rat

Abstract

It is well known that production of ROS compounds and generation of oxidative stress during diabetes are the most important mechanisms of tissue damage. The aim of this study was to examine the effects of atorvastatin treatment, as an antioxidant, to prevent the brain tissue oxidative stress in streptozotocin-induced diabetic rats. Male Wistar rats were randomly divided into four groups (five rats in each group) as followed: normal, normal treated was orally received 20 mg/kg/day atorvastatin for 30 days, diabetic group was given 40 mg/kg streptozotocin by intravenous injection and diabetic treated similar to normal treated rats. After 30 days of treatment, rats were sacrificed under deep anesthesia to remove the brain. After tissue homogenization, superoxide dismutase (SOD) and catalase (CAT) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods. In addition to increase blood glucose level in diabetic rats (78%), brain SOD and CAT activities were significantly increased compared with normal rats. Also, diabetes significantly decreased the GSH content of brain tissue by 57%, and increased the brain MDA level by 35%. Finally treatment with atorvastatin significantly decreased the augmented brain CAT activity and the MDA level during diabetes. Based on the finding of this study, diabetes-induced hyperglycemia provoked the production of free radicals in the brain tissue that leading to oxidative stress. Also, treatment with atorvastatin may have prevented from hyperglycemia-induced oxidative stress in the brain of diabetic rat.

Assessing of Antidiabetic and Ameliorative Effect of<i> </i>Lupin Seed Aqueous Extract on Hyperglycemia, Hyperlipidemia and Effect on pdx1, Nkx6.1, Insulin-1, GLUT-2 and Glucokinase Genes Expression in Streptozotocin-induced Diabetic Rats

“…Twenty male rats were injected intraperitoneal (IP) with STZ (50 mg/kg B.wt) that was dissolved previously in cold 0.1M citrate buffer (PH is 4.5) after overnight fasting [25]. Following STZ injection, 5% glucose water was given to all animals for one day to avoid hypoglycemic mortality due to leakage of insulin from damaged β cells [26].…”

Protective Role of Wheat Germ Oil against Hyperglycemia and Hyperlipidemia in Streptozotocin Induced Diabetic Rats

“…For induction of type 1 DM. Rats were fasted overnight then injected intraperitoneally (IP) with 50 mg STZ previously dissolved in freshly prepared cold 0.1 M citrate buffer (0.1 M citric acid, 0.1 M trisodium citrate, PH is 4.5) according to Mohammadi et al (2013). The animals were given 5% glucose water for 24 h following STZ injection to prevent initial drug induced hypoglycemic mortality.…”