ATP-Charged Nanoclusters Enable Intracellular Protein Delivery and Activity Modulation for Cancer Theranostics

Zhou, Zhanwei; Zhang, Qingyan; Yang, Ruoxi; Wu, Hui; Zhang, Minghua; Qian, Chenggen; Chen, Xiang‐Zhong; Sun, Minjie

doi:10.1016/j.isci.2020.100872

Cited by 19 publications

(15 citation statements)

References 36 publications

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“…After cellular internalization, the borate ester bond between PBA and alginate was broken under intracellular ATP condition, resulting in the disassembly of crosslinked polymer, cationic-to-anionic charge reversal and rapid siRNA release (owing to the electrostatic repulsion between anionic complex and negative siRNA). By using similar strategies, the same research group have constructed ATP-triggered CR-NDDSs with borate ester bonds fraction, such as HA-PEI/siRNA nano system and mPEG-b-poly(2-[(2-aminoethyl)amino]ethylaspatamide) (pDET) nano-platform [ 98 , 105 ].…”

Section: Mechanisms Of Triggered Surface Charge Conversionmentioning

confidence: 99%

Charge reversal nano-systems for tumor therapy

Zhang

Chen

et al. 2022

J Nanobiotechnol

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Surface charge of biological and medical nanocarriers has been demonstrated to play an important role in cellular uptake. Owing to the unique physicochemical properties, charge-reversal delivery strategy has rapidly developed as a promising approach for drug delivery application, especially for cancer treatment. Charge-reversal nanocarriers are neutral/negatively charged at physiological conditions while could be triggered to positively charged by specific stimuli (i.e., pH, redox, ROS, enzyme, light or temperature) to achieve the prolonged blood circulation and enhanced tumor cellular uptake, thus to potentiate the antitumor effects of delivered therapeutic agents. In this review, we comprehensively summarized the recent advances of charge-reversal nanocarriers, including: (i) the effect of surface charge on cellular uptake; (ii) charge-conversion mechanisms responding to several specific stimuli; (iii) relation between the chemical structure and charge reversal activity; and (iv) polymeric materials that are commonly applied in the charge-reversal delivery systems. Graphical Abstract

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Section: Mechanisms Of Triggered Surface Charge Conversionmentioning

confidence: 99%

Charge reversal nano-systems for tumor therapy

Zhang

Chen

et al. 2022

J Nanobiotechnol

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“…Because of the higher affinity toward ATP over that of trypsin, the polymer would desorb from the enzyme leading to the liberation of native trypsin and complete recovery of enzymatic activity. Similarly, Zhou et al [99] utilized PBA-modified polycations to tune the catalytic activity of GOx. As shown in Figure 7a, PBA was modified to polymer mPEG-b-poly(2-[(2 aminoethyl)amino]ethylaspatamide) (pDET) via amide bond, denoted as PCD.…”

Section: Tumor Therapymentioning

confidence: 99%

“…a) The mechanism of the preparation of PCD/GOx; b) The mechanism of the ATP response of PCD; c) In vivo biodistribution of 4T1-bearing mice after intravenous injection with GOx-Cy5.5 or PCD/GOx-Cy5.5; d) Representative ex-tumors imaged at day 18 (n = 5). Reproduced with permission [99]. Copyright 2020, Elsevier.…”

mentioning

confidence: 99%

Weaving Enzymes with Polymeric Shells for Biomedical Applications

Blum

et al. 2021

Advanced Materials

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the most common administration method. However, the sizes of most enzymes are smaller than the kidney filtration threshold (50-60 kDa), resulting in fast renal clearance and short half-lives. [5] Also, limited by its hydrophilicity and large molecular weight, it is difficult for exogenous enzyme macromolecules to pass through the cell membrane to effectively catalyze intracellular target reactions. [6] To date, most of the clinical protein therapeutics were explored toward extracellular targets owing to the low translocation efficiency. [7] However, for enzyme therapeutics, especially for the enzyme replacement therapy, intracellular delivery is considered critical for effective therapy. [8] Typically, even if a number of enzymes are transfected into

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“…A shield‐transport‐recover (STR) cluster can deliver when nanoclusters filled tumor sites, this promotes the intracellular transduction of enzymes. [ 160 ] Versatile and multi‐functional nanoreactors are extremely sorted for catalase based photodynamic and chemotherapy. An oncolytic nanoreactor platform can be envisaged by loaded with catalase or other oxidase enzyme to product hydrogen peroxide.…”

Section: Perspectivesmentioning

confidence: 99%

Biocompatible nanoreactors of catalase and nanozymes for anticancer therapeutics

Munjal

Dutta

2021

Nano Select

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Significant progress has laid the groundwork in understanding the fundamental role of catalase enzyme and catalase-like nanozymes, notably with the emergence of nano-bioreactors which are promoting nanomaterials-based antitumor therapeutic strategies. The radiotherapy techniques such as photodynamic, photothermal, photoacoustic and along with chemotherapy are extensively used for interrupting microenvironment by exerting to locally applied ionizingradiations. Nevertheless, tumor therapy not only deficient in therapeutic efficiency for various solid tumors but also alerts significant side effects. The convergence of nano-bioreactor driven catalase delivery at the lysosome of tumor cells with photodynamic therapy (PDT) tool along with the engineered catalase-like nanozymes enable outstanding efficacy of radiotherapy for in vivo target specific cell population. Herein, the state of the art of catalase delivering nano-bioreactors that activate the catalase delivery in tumor microenvironment (TME) are reviewed. This was linked with the functional mimic catalase-like engineered nanozymes for improving the challenges in the TME for a widerange of cell lines in vivo. Such nanoarchitecture-based strategies of catalase and synthetic nanozymes include lysosomal upregulation of H 2 O 2 in TME, image-based probing, and mechanism of catalase action for reactive oxygen species generation which are likely to amplify efficiency of cancer therapy.

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ATP-Charged Nanoclusters Enable Intracellular Protein Delivery and Activity Modulation for Cancer Theranostics

Cited by 19 publications

References 36 publications

Charge reversal nano-systems for tumor therapy

Charge reversal nano-systems for tumor therapy

Weaving Enzymes with Polymeric Shells for Biomedical Applications

Biocompatible nanoreactors of catalase and nanozymes for anticancer therapeutics

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