ATP Sulfurylase is Essential for the Utilization of Sulfamate as a Sulfur Source in the Yeast Komagataella pastoris (syn. Pichia pastoris)

Linder, Tomas

doi:10.1007/s00284-017-1276-0

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Sulfamates are thought to be assimilated through a sulfate intermediate (Fig. 1 ) as the enzyme ATP sulfurylase (encoded by the MET3 gene in yeast) is essential for the utilization of sulfamate as a sulfur source (Linder 2017 ). The enzyme responsible for desulfurization of sulfamates remains to be identified.…”

Section: Sulfonamides and Sulfamatesmentioning

confidence: 99%

“…Both human and bacterial arylsufatase-type enzymes have previously been shown to be involved in the desulfurization of sulfamate-containing polysaccharides such as heparan sulfate (Scott et al 1995 ; Myette et al 2009 ). However, the presence of arylsulfatase-family enzymes does not correlate with the ability to utilize sulfamate as a sulfur source among yeasts (Linder 2012 , 2017 ). Conversely, the utilization of sulfamate-containing polysaccharides as sulfur sources has not yet been established in fungi, which does not exclude that fungal arylsulfatase-type enzymes could play a role in the desulfurization of such compounds.…”

Section: Sulfonamides and Sulfamatesmentioning

confidence: 99%

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Assimilation of alternative sulfur sources in fungi

Linder

2018

World J Microbiol Biotechnol

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Fungi are well known for their metabolic versatility, whether it is the degradation of complex organic substrates or the biosynthesis of intricate secondary metabolites. The vast majority of studies concerning fungal metabolic pathways for sulfur assimilation have focused on conventional sources of sulfur such as inorganic sulfur ions and sulfur-containing biomolecules. Less is known about the metabolic pathways involved in the assimilation of so-called “alternative” sulfur sources such as sulfides, sulfoxides, sulfones, sulfonates, sulfate esters and sulfamates. This review summarizes our current knowledge regarding the structural diversity of sulfur compounds assimilated by fungi as well as the biochemistry and genetics of metabolic pathways involved in this process. Shared sequence homology between bacterial and fungal sulfur assimilation genes have lead to the identification of several candidate genes in fungi while other enzyme activities and pathways so far appear to be specific to the fungal kingdom. Increased knowledge of how fungi catabolize this group of compounds will ultimately contribute to a more complete understanding of sulfur cycling in nature as well as the environmental fate of sulfur-containing xenobiotics.

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Section: Sulfonamides and Sulfamatesmentioning

confidence: 99%

Section: Sulfonamides and Sulfamatesmentioning

confidence: 99%

Assimilation of alternative sulfur sources in fungi

Linder

2018

World J Microbiol Biotechnol

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“…Further addition of the sulfur atom is possible only in the form of sulfide, so sulfate and sulfite from the external environment are first reduced in the highly conservative pathway [39]. Once external sulfate enters the cell, it is metabolized into active adenosyl sulfate by ATP sulfurylase, and further converted to phosphoadenylsulfate (APS) by APS kinase.…”

Section: Introductionmentioning

confidence: 99%

Effect of Methionine on Gene Expression in Komagataella phaffii Cells

et al. 2023

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Komagataella phaffii yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in K. phaffii cells using RNA-seq analysis. Several gene groups exhibited altered expression when K. phaffii cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The AOX1 gene promoter, which is widely used for heterologous expression in K. phaffii, is downregulated in methionine-containing media. Despite great progress in the development of K. phaffii strain engineering techniques, a sensitive adjustment of cultivation conditions is required to achieve a high yield of the target product. The revealed effect of methionine on K. phaffii gene expression is important for optimizing media recipes and cultivation strategies aimed at maximizing the efficiency of recombinant product synthesis.

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“…Despite bacterial sulfate dissimilation pathway, ATP sulfurylase is involved in sulfate assimilation. This enzyme has been founded in the cells of many different organisms, such as yeast: Saccharomyces cerevisiae and Komagataella pastoris (Linder 2017), fungi: Penicillium duponti and Penicillium chrisogenum (Resonto et al 1985), plants: cabbage leaves (Osslund et al 1982), spinach (Resonto et al 1993), and soybean Glycine max (Herrmann 2014). ATP sulfurylase is involved in the transport of sulfate in the cell and in the processes of dissimilation/assimilation of sulfur-containing compounds.…”

Section: Introductionmentioning

confidence: 99%

ATP sulfurylase activity of sulfate-reducing bacteria from various ecotopes

et al. 2020

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Sulfate-reducing bacteria (SRB) are widespread in various ecotopes despite their growth and enzymatic features not compared. In this study, the enzymatic parameters of ATP sulfurylase in cell-free extracts of sulfate-reducing bacteria isolated from various ecotopes such as soils, corrosion products and human large intestine were determined. Comparative analysis of both enzyme characteristics and growth parameters were carried out and similar research has not been reported yet. The initial and maximum rates of enzymatic reaction catalyzed by ATP sulfurylase were significantly different (p < 0.05) in the bacterial strains isolated from various environmental ecotopes. The specific activity of this enzyme in sulfate-reducing bacteria was determined for corrosive and intestinal strains 0.98-1.56 and 0.98-2.26 U × mg −1 protein, respectively. The Michaelis constants were 1.55-2.29 mM for corrosive and 2.93-3.13 mM for intestinal strains and the affinity range were demonstrated. Based on cluster analysis, the parameters of physiological and biochemical characteristics of sulfate-reducing bacteria from different ecotopes are divided into 3 clusters corresponding to the location of their isolation (soils, heating systems and human intestine). Understanding the enzymatic parameters of the initial stages of sulfate consumption in the process of dissimilatory sulfate reduction will allow the development of effective methods for controlling the production of toxic metabolites, including hydrogen sulfide.

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ATP Sulfurylase is Essential for the Utilization of Sulfamate as a Sulfur Source in the Yeast Komagataella pastoris (syn. Pichia pastoris)

Cited by 7 publications

References 16 publications

Assimilation of alternative sulfur sources in fungi

Assimilation of alternative sulfur sources in fungi

Effect of Methionine on Gene Expression in Komagataella phaffii Cells

ATP sulfurylase activity of sulfate-reducing bacteria from various ecotopes

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