Atrazine degradation by a simple consortium of Klebsiella sp. A1 and Comamonas sp. A2 in nitrogen enriched medium

Yang, Chunyu; Li, Yang; Zhang, Kun; Wang, Xia; Ma, Cuiqing; Tang, Hongzhi; Xu, Ping

doi:10.1007/s10532-009-9284-9

Cited by 84 publications

(43 citation statements)

References 28 publications

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“…strains which were able to tolerate and remove lindane (c-hexachlorocyclohexane), another OP, from culture media and soil, as pure and mixed cultures. The use of microbial mixed cultures has proved to be more suitable for bioremediation of recalcitrant compounds than pure cultures because their biodiversity can enhance environmental survival and increase the number of catabolic pathways available for the biodegradation of the xenobiotic (Siripattanakul et al 2009;Yang et al 2010). In this context, the aims of this study were the selection of the most efficient actinobacteria consortium for MTX biodegradation and their application on slurry and soil systems, as potential techniques for ex situ and in situ bioremediation, respectively.…”

Section: Introductionmentioning

confidence: 99%

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Fuentes

Álvarez

Sáez

et al. 2013

Int. J. Environ. Sci. Technol.

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Methoxychlor is an organochlorine pesticide used worldwide against several insect pests, resulting in human exposure. This pesticide mimics endocrine hormone functions, interfering with normal endocrine activity in humans and wildlife. For this reason, it is imperative to develop methods to remove this pesticide from the environment, and though, bioremediation using microorganisms results as an excellent strategy. Five Streptomyces spp. strains previously isolated from organochlorine-polluted sites and capable to grow and remove methoxychlor were combined as different mixed cultures to increase methoxychlor removal. From the 39 consortia tested, one consortium (Streptomyces spp. A6, A12, A14, M7) was selected because of its high pesticide removal and specific dechlorinase activity to be assayed on slurry and soil systems. This consortium showed higher biomass values (8.3 9 10 6 ± 5.7 9 10 5 CFU mL -1 ) and methoxychlor removal (56.2 ± 2.3 %) on enriched slurry than in nonenriched slurry (7.3 9 10 5 ± 1.2 9 10 5 CFU mL -1 and 45.6 ± 7.4 % of pesticide removal). In soil systems, Streptomyces consortium showed higher growth (1.0 9 10 11 ± 5.0 9 10 10 CFU g -1 ) than in enriched slurry, although differences in methoxychlor removal between both culture conditions were not statistically significant. Therefore, the selected Streptomyces consortium may be suitable for the development of in situ (soil) and ex situ (slurry bioreactor) bioremediation methods because of their potential to remove methoxychlor from different systems.

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Section: Introductionmentioning

confidence: 99%

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Fuentes

Álvarez

Sáez

et al. 2013

Int. J. Environ. Sci. Technol.

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“…The rationale is that their biodiversity can enhance environmental survival and increase the number of catabolic pathways available for contaminant biodegradation (Smith et al 2005). For example, in atrazine degradation assays, bacterial consortia appeared to be more common and more effective than individual species (Mandelbaum et al 1993;Assaf & Turco 1994), and some consortia were reported for their metabolic cooperative actions by examining the individual contribution in atrazine degradation (Smith et al 2005;Yang et al 2010). However, there are no reports on γ-HCH degradation by consortia of actinobacteria.…”

Section: Introductionmentioning

confidence: 99%

Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains

Fuentes

Sáez

Benimeli

et al. 2011

Water Air Soil Pollut

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The current study aimed to compare lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of lindane. Furthermore, the role of bacterial consortia of lindane-degrading strains was examined in lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L -1 lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual lindane concentration and SDA and could be a promising tool for lindane biodegradation.

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“…Apart from members of the phylum Acidobacteria, all of the genera that are indicative for HDO degradation have been reported to persist or even degrade pesticides in their respective environments (28,30,34,38). However, the analyzed pesticides and environmental conditions in these studies varied, and none of these pesticides had a chemical structure similar to that of HDO or potential metabolites.…”

Section: Discussionmentioning

confidence: 97%

Biodegradation of a Biocide (Cu- N -Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community

Jakobs-Schönwandt

Mathies

Abraham

et al. 2010

Appl Environ Microbiol

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The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled ( 13 C) and nonlabeled ( 12 C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the ␦ 13 C content of respired CO 2 , as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the 13 CO 2 concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C 19:0 d8,9, C 18:0 , C 18:1 7, C 18:2 6,9, C 17:1 d7,8, C 16:0 , and C 16:1 7 were highly enriched in their ␦ 13 C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.

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Atrazine degradation by a simple consortium of Klebsiella sp. A1 and Comamonas sp. A2 in nitrogen enriched medium

Cited by 84 publications

References 28 publications

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains

Biodegradation of a Biocide (Cu- N -Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community

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