Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

Alghilan, Maryam A.; Windsor, L. Jack; Palasuk, Jadesada; Yassen, Ghaeth H.

doi:10.1111/iej.12669

Cited by 45 publications

(46 citation statements)

References 37 publications

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“…This antibiofilm effect was exactly similar to 20 mg mL −1 clindamycin, so that the same concentration of clindamycin alone was showed the same efficacy in comparison with TAP. The common used concentration of TAP in the clinical condition is 1000 mg mL −1 that recently has been shown toxic for apical papillae and dental pulp stem cells . Recent studies indicated highly unfavorable effect of TAP on surface roughness and the colour of dentine , so a reduced concentration of TAP in our study was used.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: An in vitro study

Zargar

Abadi²,

Sabeti

et al. 2018

Aust Endodontic J

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This study compared the antibacterial effect of 2% clindamycin and 2% and 100% concentration of triple antibiotic paste (TAP) on an Enterococcus faecalis biofilm. Dentinal tubules of 100 root specimens were infected and randomly assigned to five groups. A total of 1000 mg mL of TAP, 20 mg mL of TAP and clindamycin, calcium hydroxide or methylcellulose (control) were placed in the root canal for 1 week. After treatment, dentine shavings were collected from 200 and 400 μm dentine depth and the number of colony-forming units (CFU) per mg was determined. Reduction in viable bacteria in first three groups was significantly better than calcium hydroxide and control groups. However, the antimicrobial effectiveness among these three groups was not significantly different from each other. There was no significant difference between data at 200 and 400 μm in all groups except the Ca(OH) group. The antibiofilm effect of clindamycin was comparable with TAP, so it may be used instead of TAP.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: An in vitro study

Zargar

Abadi²,

Sabeti

et al. 2018

Aust Endodontic J

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show abstract

“…Even at low concentrations, TAP has unwanted effects on the proliferative capacity and mineralized matrix formation of dental pulp cells and apical papilla cells [ 50 ]. According to several studies, a concentration of 0.125 mg/mL of TAP has no cytotoxic effect on the stem cells; thus, it is expected that a concentration of the antibiotic combination with the lowest possible side effects and adequate ability for removing microorganisms, particularly Enterococcus faecalis ( E. faecalis ) must be used during endodontic treatments [ 50 51 ]. The proper and careful combination of the 3 antibiotics has shown to create an appropriate environment for the cell attachment to occur, and to have enough dislocation resistance [ 52 ] for mineral trioxide aggregate (MTA) or calcium enriched mixture (CEM) cement as the bio-regenerative dressing.…”

Section: Reviewmentioning

confidence: 99%

Triple antibiotic paste: momentous roles and applications in endodontics: a review

2018

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This study investigated the latest findings and notions regarding ‘triple antibiotic paste’ (TAP) and its applications in dentistry, particularly endodontics. TAP is a combination of 3 antibiotics, ciprofloxacin, metronidazole, and minocycline. Despite the problems and pitfalls research pertaining to this paste has unveiled, it has been vastly used in endodontic treatments. The paste's applications vary, from vital pulp therapy to the recently introduced regeneration and revascularisation protocol. Studies have shown that the paste can eliminate the root canal microorganisms and prepare an appropriate matrix for further treatments. This combination is able to remove diverse groups of obligate and facultative gram-positive and gram-negative bacteria, providing an environment for healing. In regeneration protocol cases, this allows the development, disinfection, and possible sterilization of the root canal system, so that new tissue can infiltrate and grow into the radicular area. Moreover, TAP is capable of creating a discipline in which other wanted and needed treatments can be successfully performed. In conclusion, TAP, as an antibacterial intracanal medication, has diverse uses. Nevertheless, despite its positive effects, the paste has shown drawbacks. Further research concerning the combined paste and other intracanal medications to control microbiota is a must.

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“…Indeed, using current techniques, it appears that revitalization will at best stimulate repair, not regeneration with minimal gain in root length over traditional apexification techniques [7, 35]. As a result, specific attention was directed to developing methods, which harness the fossilized bioactive dentin matrix components, including GFs and other signaling molecules, with irrigants, medicaments, and materials [40–43]. Additionally, new functionalized scaffolds are being investigated as a mechanism for controlled delivery of angiogenic and other exogenous GFs to support and improve the regenerative response [31, 44].…”

Section: Reviewmentioning

confidence: 99%

Growth Factors and Cell Homing in Dental Tissue Regeneration

Duncan

Kobayashi

Shimizu

2018

Curr Oral Health Rep

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Purpose of Review To summarize current views on the role and therapeutic potential of growth factors (GFs) within endodontic cell homing. Recent Findings Cell homing/revitalization techniques aim to regenerate dentin and pulp using endogenous cells. Clinically, revitalization has successfully created new vital tissue in necrotic permanent teeth with an open apex; however, there is no evidence of new odontoblasts, pulp tissue, or predictable extension in root length. Although the response is reparative rather than regenerative, exciting opportunities to improve these biologically-based strategies remain by (1) efficiently sequestering dentin-matrix-components (DMCs) using irrigants and dental materials (2) designing next-generation GF-releasing scaffold materials and (3) utilizing other sources of GF such as cells and plasma-rich plasma and plasma-rich fibrin. Summary GFs can promote reparative-dentinogenesis and pulp-like tissue formation. The future development and clinical approval of GF-functionalized-scaffolds is a priority; however, current focus should be to harness DMCs and target the interaction of stem cells and GFs.

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Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

Abstract: Introduction To investigate the attachment and proliferation of dental pulp stem cells (DPSC)

Cited by 45 publications

References 37 publications

Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: An in vitro study

Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: An in vitro study

Triple antibiotic paste: momentous roles and applications in endodontics: a review

Growth Factors and Cell Homing in Dental Tissue Regeneration

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