Attachment of Arcobacter butzleri, a New Waterborne Pathogen, to Water Distribution Pipe Surfaces

Assanta, Mafu Akier; Roy, Denis; Lemay, Marie-Josée; Montpetit, Diane

doi:10.4315/0362-028x-65.8.1240

Cited by 87 publications

(60 citation statements)

References 29 publications

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“…These considerations together with the ability of A. butzleri to adhere to different surfaces (23) and to form biofilm (16,24) suggest two main sources of contamination in the investigated cheese factory: contaminated raw milk and resident strains able to survive in the wet dairy environment, characterized by large amounts of water and abundant organic material known to enhance the survival of A. butzleri (10).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Giacometti

Lucchi

Manfreda

et al. 2013

Appl Environ Microbiol

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bThe present study aimed to investigate the presence, distribution, and persistence of Arcobacter spp. in an artisanal dairy plant and to test the isolates to determine their different genotypes in the processing plant and in foods. Samples were collected in an artisanal cheese factory on four occasions between October and December 2012. Food samples (raw milk, ricotta cheese, mozzarella cheese, and conditioning liquid), water samples, and environmental samples were analyzed by the culture method; isolates were identified by multiplex PCR and genotyped by pulsed-field gel electrophoresis (PFGE) analysis. Arcobacter butzleri was isolated from 29 out of 59 samples (46.6%), 22 of which were from environmental samples and 7 of which were from food samples. Cluster analysis divided the strains into 47 PFGE patterns: 14 PFGE clusters and 33 unique types. Our findings indicate that the plant harbored numerous A. butzleri pulsotypes and that the manual cleaning and sanitation in the studied dairy plant do not effectively remove Arcobacter. The recurrent isolation of A. butzleri suggests that the environmental conditions in the dairy plant constitute a good ecological niche for the colonization of this microorganism. In some cases, the presence of indistinguishable strains isolated from the same facilities on different sampling days showed that these strains were persistent in the processing environment. Interest in arcobacters in veterinary and human public health has increased from the first report of the isolation of arcobacters from food of animal origin; since then, studies worldwide have reported the occurrence of arcobacters on food and in food production animals and have highlighted possible routes of transmission, especially for Arcobacter butzleri, to the human population (1).Arcobacter butzleri is the most important and prevalent species of the genus and has been classified as a serious hazard to human health by the International Commission on Microbiological Specifications for Foods (2) and as a significant zoonotic pathogen (3). A few surveys have investigated the presence of Arcobacter spp. in bulk tank raw cow's milk, reporting prevalence rates of 46% in Northern Ireland (4), 5.8% in Malaysia (5), and 48% in Italy (6). In Italy, A. butzleri was isolated in fecal samples and in-line milk filters on a water buffalo (WB) dairy farm (7,8). Consumption of Arcobacter-contaminated food or water is regarded as the most probable route of transmission to humans and animals (9) following fecal contamination during the various stages of production processes (10). In the Mediterranean countries, there is a widespread tradition of raw milk cheese production, including, particularly in Italy, WB cheeses. A. butzleri demonstrated the ability to survive during the production process and shelf life of WB mozzarella cheese (11), and raw milk cheeses may be considered a potential source of human infection even though A. butzleri has not been isolated from dairy products. A. butzleri contamination of food processing plants may...

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Section: Discussionmentioning

confidence: 99%

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Giacometti

Lucchi

Manfreda

et al. 2013

Appl Environ Microbiol

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“…Besides environmental sources, researchers have observed Arcobacter spp. such as Arcobacter butzleri, A. cryaerophilus, and A. skirrowii in patients with diarrhea and/or food-borne illness (13,30,33,49,60), which has led to these microbes being characterized as potential food-and waterborne pathogens (1,26). Moreover, the presence of Arcobacter in livestock and processed meat (28,29,48) suggests that animals may also serve as possible reservoirs for Arcobacter species.…”

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confidence: 99%

Arcobacter in Lake Erie Beach Waters: an Emerging Gastrointestinal Pathogen Linked with Human-Associated Fecal Contamination

Lee¹,

Agidi²,

Marion³

et al. 2012

Appl Environ Microbiol

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The genus Arcobacter has been associated with human illness and fecal contamination by humans and animals. To better characterize the health risk posed by this emerging waterborne pathogen, we investigated the occurrence of Arcobacter spp. in Lake Erie beach waters. During the summer of 2010, water samples were collected 35 times from the Euclid, Villa Angela, and Headlands (East and West) beaches, located along Ohio's Lake Erie coast. After sample concentration, Arcobacter was quantified by realtime PCR targeting the Arcobacter 23S rRNA gene. Other fecal genetic markers (Bacteroides 16S rRNA gene [HuBac], Escherichia coli uidA gene, Enterococcus 23S rRNA gene, and tetracycline resistance genes) were also assessed. Arcobacter was detected frequently at all beaches, and both the occurrence and densities of Arcobacter spp. were higher at the Euclid and Villa Angela beaches (with higher levels of fecal contamination) than at the East and West Headlands beaches. The Arcobacter density in Lake Erie beach water was significantly correlated with the human-specific fecal marker HuBac according to Spearman's correlation analysis (r ‫؍‬ 0.592; P < 0.001). Phylogenetic analysis demonstrated that most of the identified Arcobacter sequences were closely related to Arcobacter cryaerophilus, which is known to cause gastrointestinal diseases in humans. Since human-pathogenic Arcobacter spp. are linked to human-associated fecal sources, it is important to identify and manage the human-associated contamination sources for the prevention of Arcobacter-associated public health risks at Lake Erie beaches.

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“…Arcobacter have been identified in water distribution pipe biofilms (Assanta et al, 2002) and on slaughter equipment in poultry abattoirs (Houf et al, 2002;Ferreira et al, 2013). This, coupled with the observation that Arcobacter can survive long periods in water (Van Driessche and Houf, 2008), or even replicate at slaughter and refrigeration temperatures 4 to 10 °C (Kjeldgaard et al, 2009) could place it in a very different level of risk compared to the other campylobacters.…”

Section: Watermentioning

confidence: 99%

“…Arcobacter has been shown to possess the ability to form biofilms (Fernandez et al, 2008;Kjeldgaard et al, 2009;Ferreira et al, 2013) and has been identified in biofilms in drinking water distribution pipes (Assanta et al, 2002), which has the potential to contribute to gastrointestinal illness in people who consume such water.…”

Section: Drinking Watersmentioning

confidence: 99%

Arcobacter

Banting¹,

Salvat²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Attachment of Arcobacter butzleri, a New Waterborne Pathogen, to Water Distribution Pipe Surfaces

Cited by 87 publications

References 29 publications

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Arcobacter in Lake Erie Beach Waters: an Emerging Gastrointestinal Pathogen Linked with Human-Associated Fecal Contamination

Arcobacter

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