Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line

Oh, Yuri; Lim, Hye‐Won; Huang, Yuhua; Kwon, Hee-Souk; Jin, Chang Duck; Kim, Kyunghoon; Lim, Chang‐Jin

doi:10.1016/j.jphotobiol.2016.08.026

Cited by 32 publications

(28 citation statements)

References 40 publications

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“…As previously described [ 22 ], ARE was prepared as follows. Dried A. rugosa leaves, ground under liquid nitrogen, were extracted under reflux by placing in a water bath at 90 °C for 4 h. After being filtered through a filter paper, the hot water extract was evaporated to dryness in a freeze dryer, and the extract powder was designated as ARE.…”

Section: Methodsmentioning

confidence: 99%

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Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes

Shin

Lee²,

Huang

et al. 2018

BMC Complement Altern Med

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BackgroundAgastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-induced photooxidative stress. The present work aimed to examine whether probiotic bacterial fermentation would potentiate the skin anti-photoaging activity of ARE or not, by comparing the protective properties of ARE and corresponding fermented extract (ARE-F) against UV-B radiation-induced photooxidative stress in HaCaT keratinocytes.MethodsARE-F was produced from ARE by the fermentation with Lactobacillus rhamnosus HK-9, a type of Gram-positive probiotic bacterial strain. Anti-photoaging activities were evaluated by analyzing reactive oxygen species (ROS), promatrix metalloproteinases (proMMPs), total glutathione (GSH) and total superoxide dismutase (SOD) in UV-B-irradiated HaCaT keratinocytes. Antiradical activity was determined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay.ResultsARE-F contained higher attenuating activity on the UV-B-induced ROS generation than ARE. Similarly, ARE-F was able to diminish the UV-B-induced proMMP-9 and -2 more effectively than ARE. ARE-F displayed higher tendencies to augment the UV-B-reduced total GSH content and SOD activity than ARE. However, there were no significant difference between ARE and ARE-F in ABTS radical scavenging activities.ConclusionsThe findings suggest that the UV-B radiation-protective activity of ARE is enhanced by probiotic bacterial fermentation, which might improve the therapeutic and cosmetic values of A. rugosa leaves.

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Section: Methodsmentioning

confidence: 99%

“…Throughout the previous work, ARE was reported to have an attenuating potential against the UV-B-induced photoaging of human skin [ 22 ]. In the present work, it is demonstrated that the skin anti-photoaging activity of ARE can be potentiated by probiotic bacterial fermentation.…”

Section: Introductionmentioning

confidence: 99%

Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes

Shin

Lee²,

Huang

et al. 2018

BMC Complement Altern Med

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“…Agastache rugosa Kuntze (Korean mint) is a perennial herb and well known as Bangah in the southern region of Korea [16]. Tilianin is a major constituent of A. rugosa and has been associated with numerous pharmacologic effects, such as antioxidant, antiatherogenic, antitumor, and antiinflammatory activities [17,18].…”

Section: Introductionmentioning

confidence: 99%

Tilianin Inhibits MUC5AC Expression Mediated Via Down-Regulation of EGFR-MEK-ERK-Sp1 Signaling Pathway in NCI-H292 Human Airway Cells

Song

Ryu

et al. 2017

Journal of Microbiology and Biotechnology

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In the human airway, mucus exists to protect the respiratory system as a primary barrier of the innate immune system. However, hyperexpressed mucus limits airflow, resulting in a decrease of lung function. Among more than 20 mucin family members, MUC5AC and MUC5B are major glycoproteins in human airway mucus. The epidermal growth factor receptor (EGFR) signaling pathway is one of the mechanisms of these mucins expression and specificity protein-1 (Sp1) transcription factor is the downstream signal of this pathway, playing pivotal roles in mucin expression. Even though there are some drugs for treating mucus hypersecretion, no drug has proven effects on humans. We found that the flavonoid tilianin regulated MUC5AC expression and also inhibited Sp1 phosphorylation. In this study, we investigated how tilianin would modulate EGFR signaling and regulate mucin production. In conclusion, tilianin inhibited MUC5AC expression mediated via modulating the EGFRMEK-ERK-Sp1 signaling pathway in NCI-H292 human airway epithelial cells. This study may provide the basis for the novel treatment of mucus hypersecretion.

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“…The antioxidant effect of AR was dose-dependent and led to increased HO-1 protein and enzyme activity, and protected cells from H 2 O 2 -induced cytotoxicity [27]. Such response also attenuated UVB-induced photoaging by upregulating anti-oxidant enzymes [28] and reducing the production of ROS [29]. Our results showed that compared with the HFD group, the levels of SOD and GSH in the liver of AR group were significantly increased, while the levels of MDA and ROS were significantly decreased in hepatocytes (Fig.…”

Section: Discussionmentioning

confidence: 95%

Agastache rugosa alleviates the multi-hit effect on hepatic lipid metabolism, inflammation and oxidative stress during nonalcoholic fatty liver disease

Cui¹,

Chang²,

Wang³

et al. 2020

Preprint

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Background Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease, and has high rates of morbidity and mortality worldwide. Agastache rugosa (AR) possesses unique anti-oxidant, anti‑inflammatory and anti-atherosclerosis characteristics. Methods To investigate the effects and the underlying mechanism of AR on NAFLD, we fed mice a high-fat diet (HFD) to establish NAFLD model of mice in vivo experiment and induced lipidosis in AML12 hepatocytes through a challenge with free fatty acids (FFA) in vitro. The contents of total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver homogenates were measured. Pathological changes in liver tissue were evaluated by HE staining. Oil red O staining was used to determine degree of lipid accumulation in liver tissue, and Western blot was used to detect abundance of inflammation-, lipid metabolism- and endoplasmic reticulum stress-related proteins. Results Supply of AR alleviated accumulation of lipid in hepatocytes induced by HFD in vivo and challenged with free fatty acids (FFA) in vitro. Compared with the HFD group, supplementing AR decreased p-NF-κB/NF-κB and p-IκB/IκB protein and inhibited abundance of PERK, IRE1 and ATF6 (P < 0.05). Furthermore, AR reduced lipid accumulation within hepatocytes by downregulating abundance of SREBP, ACC1 and FAS (P < 0.05). Supply of AR significantly attenuated ROS accumulation and MDA production by improving antioxidant enzymatic activity including SOD and GSH (P < 0.01). Conclusion Supply of AR attenuates disordered lipid metabolism and enhances the antioxidative defense associated with NAFLD induced by HFD in mice. Results underscore the potential of plants used in traditional Chinese medicine to achieve pharmacological benefits through a multi-tier cellular response.

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Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line

Cited by 32 publications

References 40 publications

Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes

Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes

Tilianin Inhibits MUC5AC Expression Mediated Via Down-Regulation of EGFR-MEK-ERK-Sp1 Signaling Pathway in NCI-H292 Human Airway Cells

Agastache rugosa alleviates the multi-hit effect on hepatic lipid metabolism, inflammation and oxidative stress during nonalcoholic fatty liver disease

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