Attenuation of cryopreservation-induced oxidative stress by antioxidant: Impact of Coenzyme Q10 on the quality of post-thawed buck spermatozoa

Yousefian, Iman; Emamverdi, Mojtaba; Karamzadeh-Dehaghani, Amir; Sabzian-Melei, Razieh; Zhandi, Mahdi; Zare-Shahneh, Ahmad

doi:10.1016/j.cryobiol.2018.02.005

Cited by 24 publications

(15 citation statements)

References 28 publications

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“…Studies that report that antioxidants obtained from plants maintain chromatin integrity (Avdatek et al, 2018; Awan et al, 2018) and that there is a positive relationship between decreasing oxidative stress and decreasing chromatin damage (Losano et al, 2018) supported our findings. In another study conducted in rams, in accordance with our results, addition of 1 μM of coenzyme Q10, a lipophilic antioxidant, caused the sperm chromatin integrity to increase and MDA activity to decrease (Yousefian et al, 2018). On the contrary, there are studies expressing that quercetin addition has no healing effect on sperm chromatin damage (Seifi‐Jamadi, Kohram, Shahneh, Ansari, & Macías‐García, 2016).…”

Section: Discussionsupporting

confidence: 88%

Red pine ( Pinus brutia Ten ) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

et al. 2020

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This study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200, 100, 50 and 25 µg/ml) and P free (control; C) up to a final concentration of 16 × 106 per straw. All specimens were equilibrated for a period of 4 hr at a temperature of 4°C, following which they were filled in 0.25‐ml French straws and frozen. Addition of P resulted in favourable tail length in comparison with C (p < .05). The lowest malondialdehyde levels and the highest glutathione levels were detected in all P groups (p < .05). Supplementation with P did not show advanced results in terms of total, progressive sperm motility and total abnormality in comparison with C (p > .05). In conclusion, it has been shown that although P added to a Tris extender does not have a positive effect on sperm motility, it prevents chromatin damage by reducing oxidative stress, in addition to reducing head abnormalities when used at the amount of 50 μg/ml.

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Section: Discussionsupporting

confidence: 88%

Red pine ( Pinus brutia Ten ) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

et al. 2020

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“…However, further studies should be conducted to elucidate why the intense impact of freezing-thawing process on the mitochondrial activity in buck sperm cannot be counteract by the mentioned antioxidants. By contrast, other authors observed a significant increase in mitochondrial potential when cysteine, coenzyme Q 10, lycopene or alpha-lipoic acid were used [10,11,65].…”

Section: Discussionmentioning

confidence: 71%

“…By contrast, when spermatozoa are exposed to stress conditions, endogenous antioxidants cannot counteract the excess of free radicals; thus, the addition of exogenous antioxidants may be essential to preserve the quality of these cells. In this sense, several studies have evaluated the use of exogenous antioxidants in goat sperm [3,[8][9][10][11]. In this framework, a large number of natural compounds has been tested in cell cultures assessing the antioxidant, anti-inflammatory or chelating properties of antioxidants, in recent years, e.g., mentha [12], Feijoa sellowiana [13], grapes [14], or olives [15].…”

Section: Introductionmentioning

confidence: 99%

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Arbulu

González

Bermúdez-Oria

et al. 2021

Animals

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The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian inference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mitochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 µg/mL and DHPG at 30 µg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively.

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“…In cryopreservation, antioxidants can reduce oxidative stress (Mathew et al, 2019), regulate the synthesis of mitochondrial proteins (Banday et al, 2017), decrease ROS production (Zhu et al, 2019), clear intracellular ROS (Len et al, 2019), enhance the activity of antioxidant enzyme (Azadi et al, 2017), resist to LPO and DNA fragmentation (Yousefian et al, 2018). Specifically, for germ cells such as sperm, antioxidants can increase motility parameters (Toker et al, 2016), acrosomal integrity (Lone et al, 2018), mitochondrial membrane potential (Fontoura et al, 2017) and pregnancy rates (Ren et al, 2018).…”

Section: Positive Effectsmentioning

confidence: 99%

The Feasibility of Antioxidants Avoiding Oxidative Damages from Reactive Oxygen Species in Cryopreservation

Liu

et al. 2021

Front. Chem.

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Cryopreservation prolongs the storage time of cells and plays an important role in modern biology, agriculture, plant science and medicine. During cryopreservation, cells may suffer many damages, such as osmotic dehydration, large ice puncture and oxidative damages from reactive oxygen species (ROS). Classic cryoprotectants (CPAs) are failing to dispose of ROS, while antioxidants can turn ROS into harmless materials and regulate oxidative stress. The combination of antioxidants and CPAs can improve the efficiency of cryopreservation while negative results may occur by misuse of antioxidants. This paper discussed the feasibility of antioxidants in cryopreservation.

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Attenuation of cryopreservation-induced oxidative stress by antioxidant: Impact of Coenzyme Q10 on the quality of post-thawed buck spermatozoa

Cited by 24 publications

References 28 publications

Red pine ( Pinus brutia Ten ) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

Red pine ( Pinus brutia Ten ) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

The Feasibility of Antioxidants Avoiding Oxidative Damages from Reactive Oxygen Species in Cryopreservation

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