Augmentin® as an alternative antibiotic for growth suppression of Agrobacterium for tomato (Lycopersicon esculentum) transformation

Ieamkhang, Supaporn; Chatchawankanphanich, Orawan

doi:10.1007/s11240-005-0416-6

Cited by 22 publications

(11 citation statements)

References 21 publications

(32 reference statements)

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“…At this level plant regeneration from the leaf explants of P. acerifolia was completely inhibited. The A. tumefaciens on infected Platanus leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L -1 Timentin, which was nearly in line with the results obtained by Ieamkhang and Chatchawankanphanich (2005).…”

Section: Effect Of Antibiotics On Suppression Of Agrobacterium Tumefasupporting

confidence: 89%

“…The suppression of bacterial growth was monitored by measuring the absorbance of bacterial culture at OD 600 (A) and estimation of Agrobacterium cell numbers as colony (B). genetic transformations should be highly effective, have no negative effect on plant growth and regeneration, should be stable in culture and inexpensive (Ieamkhang et al, 2005). Currently, carbenicillin and cefotaxime are the most commonly used antibiotics for such a purpose (Cheng et al, 1998).…”

Section: Effect Of Antibiotics On Suppression Of Agrobacterium Tumefamentioning

confidence: 99%

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Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Fang

et al. 2007

For. Stud. China

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The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L -1 . Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L -1 ; however, 300 mg·L -1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% (control) to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L -1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L -1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L -1 is the preferred application in A. tumefaciens-mediated transformation of P. acerifolia.

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Section: Effect Of Antibiotics On Suppression Of Agrobacterium Tumefasupporting

confidence: 89%

Section: Effect Of Antibiotics On Suppression Of Agrobacterium Tumefamentioning

confidence: 99%

Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Fang

et al. 2007

For. Stud. China

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“…Timentin TM is one of the most expensive antibiotics used widely in transformation studies (Ieamkhang et al 2005;Slater et al 2011;Thiruvengadam et al 2011). Clavamox Ò is relatively inexpensive and appears to work effectively at a low dose (250 mg l -1 ).…”

Section: Selection For Transformationmentioning

confidence: 99%

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Ren

Bang

Curtis

et al. 2011

Plant Cell Tiss Organ Cult

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“…The use of AugmentinÒ was found beneficial in Agrobacterium-mediated transformation systems. After cocultivation AugmentinÒ was used in the medium to suppress the bacterial growth, but at the same time it supported growth of plant system with regard to cell division (plant growth) and cell differentiation (shoot organogenesis) as reported in many plant systems (Ieamkhang and Chatchawankanphanich 2005). Other antibiotics like ampicillin and antifungal agents like benomyl had also been used to control contamination in Annonaceae (Santana et al 2003).…”

Section: Identification Of Organism and Microscopic Examinationmentioning

confidence: 99%

Establishment of long-term proliferating shoot cultures of elite Jatropha curcas L. by controlling endophytic bacterial contamination

Misra

Gupta

Toppo

et al. 2009

Plant Cell Tiss Organ Cult

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Leaf explants of the second or third node were collected from field-grown elite Jatropha curcas trees and incubated in Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium supplemented with growth regulators. Direct shoot organogenesis was induced when explants were incubated in a medium containing 0.5 mg l -1 benzyladenine (BA) and 0.1 mg l -1 indolebutyric acid (IBA). A maximum of seven shoot buds differentiated within 6 weeks of culture incubation. Indirect shoot organogenesis was obtained when explants were incubated in the medium supplemented with 0.5 mg l -1 BA along with 1.0 mg l -1 each of 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA). A pulse treatment of 0.5 mg l -1 thidiazurone (TDZ) and 0.1 mg l -1 IBA for 5 days was necessary for shoot organogenesis in green compact callus before subculture into 0.5 mg l -1 BA and 0.1 mg l -1 IBA containing medium. Leaf explants of J. curcas, collected from the field, contained endophytic bacterial contamination, which expressed itself after 2-3 subcultures. These bacteria were cultured and identified as Enterobacter ludwigii. After staining, these were found as gram-negative bacteria. Their sensitivity against different antibiotics has been tested by culturing them with different antibiotic stabs for 72 h. Finally, AugmentinÒ was found as the most effective and suitable antibiotic which not only controlled the bacteria within 2-3 subcultures but also supported the regeneration system and growth of the regenerated shoots and such cultures have been grown for a long-term of over 2 years without any contamination.

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Augmentin® as an alternative antibiotic for growth suppression of Agrobacterium for tomato (Lycopersicon esculentum) transformation

Abstract: Augmentin Ò was used as an alternative antibiotic for suppressing Agrobacterium tumefaciens during tomato (Lycopersicon esculentum) transformation. The efficiency of Augmentin compared with Timentin Ò

Cited by 22 publications

References 21 publications

Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Establishment of long-term proliferating shoot cultures of elite Jatropha curcas L. by controlling endophytic bacterial contamination

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