Autoantibodies to Mitotic Apparatus: Association with Other Autoantibodies and Their Clinical Significance

Abstract

The most important mitotic apparatus (MA) antigens are centrosome (CE), nuclear mitotic apparatus (NuMA-1, NuMA-2), midbody, and centromere F (CENP-F). We studied associations of anti-MA antibodies with other autoantibodies and their clinical significance. A total of 6270 patients were studied for the presence of anti-MA antibodies on HEp-2 cells. Sera positive for anti-MA were tested for anti-extractable nuclear antigens (ENA) antibodies. Anti-MA antibodies were detected in 56 (45 females and 11 males) of 6270 sera (0.9%). Of these 56, NuMA-1 was found in 23, NuMA-2 in 7, CE in 20, CENP-F in 5, and CENP-F/centrosome in 1 case. Anti-NuMA-1 were associated with anti-ENA antibodies (p < 0.001). Diagnoses were established in 43/56 patients: 22 connective tissue diseases, 7 infections, 6 autoimmune hepatitis, 3 vasculitis, 3 primary antiphospholipid syndrome, 1 malignancy, and 1 fever of unknown origin. The differential diagnosis of anti-NuMA-1-positive patients must include Sjögren's syndrome, while patients with anti-CE antibodies must be observed for HCV infection.

Clinical characteristics of patients with autoimmune hepatitis seropositive for anticentromere antibody

“…Patients with PBC seropositive for ACA often seem to develop portal hypertension 7 or liver failure 8 . ACA is occasionally found in the sera of patients with type 1 autoimmune hepatitis (AIH) 2,5,6,9,10 or even in the sera of patients with hepatitis C virus (HCV)‐related chronic liver disease (CLD) 11,12 …”

“…8 ACA is occasionally found in the sera of patients with type 1 autoimmune hepatitis (AIH) 2,5,6,9,10 or even in the sera of patients with hepatitis C virus (HCV)-related chronic liver disease (CLD). 11,12 We have previously shown that patients with AIH seropositive for ACA are likely to have milder liver damage and a more favorable prognosis. 13 However, a case report indicating that an AIH patient associated with CREST syndrome, who rapidly developed hepatic failure, was later documented.…”

Anti‐Mitotic Spindle Apparatus Antoantibodies: Prevalence and Disease Association in Chinese Population

“…Anti‐MSA antibodies are reported to be associated with a wide variety of autoimmune diseases including RA, SS, SLE, MCTD, polyarteritis nodosa, polymyositis, and Hashimoto's thyroiditis . Our study revealed that 72.7% (16/22) of anti‐MSA‐positive patients in Chinese had autoimmune CTDs, mostly SS, RA, and SLE.…”

Clinical Characteristics of Patients with Hepatitis C Virus-Related Chronic Liver Disease Seropositive for Anticentromere Antibody

“…To date, there have been very few reports on patients with HCV-related CLD seropositive for ACA [25,40]. To the best of our knowledge, this is the first study that has carried out detailed analyses of clinical parameters in patients with HCV-related CLD seropositive for ACA.…”