Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative

Lyngstadaas, Ståle Petter; Lundberg, Eva; Ekdahl, Helena; Andersson, Christer; Gestrelius, Stina

doi:10.1034/j.1600-051x.2001.028002181.x

Cited by 235 publications

(282 citation statements)

References 27 publications

Supporting

Mentioning

252

Contrasting

Unclassified

Order By: Relevance

“…Yet, little is known about the critical factors promoting MSCs expansion in the co-culture system. Tendon/ligament cells were reported to secrete a number of growth factors when healing or stimulated, such as IGF, TGF-b, FGF-2 and PDGF (Lyngstadaas et al 2001;Dahlgren et al 2005), among which FGF-2, PDGF and BMP-2, a member of TGF-b superfamily, have been illustrated to promote MSCs division in vitro (Martin et al 1997;Liu et al 2009;Choi et al 2008;Tamama et al 2006). In our co-culture system, the exact factors playing the critical role in MSCs proliferation need to be further clarified.…”

Section: Discussionmentioning

confidence: 99%

Indirect co-culture with tenocytes promotes proliferation and mRNA expression of tendon/ligament related genes in rat bone marrow mesenchymal stem cells

Luo

Song

et al. 2009

Cytotechnology

View full text Add to dashboard Cite

Recent evidences have suggested that humoral factors released from the appropriate cocultured cells influenced the expansion and differentiation of mesenchymal stem cells (MSCs). However, little is known about the proliferation and differentiation of MSCs subjected to co-culture condition with tenocytes. In this study, we aimed to establish a coculture system of MSCs and tenocytes and investigate the proliferation and tendon/ligament related gene expression of MSCs. MTT assay was used to detect the expansion of MSCs. Semi-quantitative RT-PCR was performed to investigate the expression of proliferation associated c-fos gene and tendon/ligament related genes, including type I collagen (Col I), type III collagen (Col III), tenascin C and scleraxis. Significant increase in MSCs expansion was observed after 3 days of co-culture with tenocytes. The c-fos gene expression was found distinctly higher than for control group on day 4 and day 7 of co-culture. The mRNA expression of four tendon/ligament related genes was significantly up-regulated after 14 days of co-culture with tenocytes. Thus, our research indicates that indirect coculture with tenocytes promotes the proliferation and mRNA expression of tendon/ligament related genes in MSCs, which suggests a directed differentiation of MSCs into tendon/ligament.

show abstract

Section: Discussionmentioning

confidence: 99%

Indirect co-culture with tenocytes promotes proliferation and mRNA expression of tendon/ligament related genes in rat bone marrow mesenchymal stem cells

Luo

Song

et al. 2009

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…This finding corroborates with other EMD related studies. 23 EMD medium led to a higher BSP gene expression irrespective of stretching. Collagen I expression was lower in the stretched group, but this was compensated by the effect of EMD.…”

Section: D Model To Study the Regeneration Of The Pdlmentioning

confidence: 85%

A Three-Dimensional Cell Culture Model to Study the Mechano-Biological Behavior in Periodontal Ligament Regeneration

Oortgiesen

Bronckers

et al. 2012

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Periodontitis is a disease affecting the supporting structures of the teeth, which can eventually result in tooth loss. A three-dimensional (3D) tissue culture model was developed that may serve to grow a 3D construct that not only transplants into defective periodontal sites, but also allows to examine the effect of mechanical load in vitro. In the current in vitro study, green fluorescent protein labeled periodontal ligament (PDL) cells form rat incisors were embedded in a 3D matrix and exposed to mechanical loading alone, to a chemical stimulus (Emdogain; enamel matrix derivative [EMD]) alone, or a combination of both. Loading consisted of unilateral stretching (8%, 1 Hz) and was applied for 1, 3, or 5 days. Results showed that PDL cells were distributed and randomly oriented within the artificial PDL space in static culture. On mechanical loading, the cells showed higher cell numbers. Moreover, cells realigned perpendicular to the stretching force depending on time and position, with great analogy to natural PDL tissue. EMD application gave a significant effect on growth and upregulated bone sialoprotein (BSP) and collagen type-I (Col-I), whereas Runx-2 was downregulated. This implies that PDL cells under loading might tend to act similar to bone-like cells (BSP and Col-I) but at the same time, react tendon like (Runx-2). The combination of chemical and mechanical stimulation seems possible, but does not show synergistic effects. In this study, a new model was successfully introduced in the field of PDLrelated regenerative research. Besides validating the 3D model to mimic an authentic PDL space, it also provided a useful and well-controlled approach to study cell response to mechanical loading and other stimuli.

show abstract

“…TGF-β 1 is known to be related to the mitogenesis of cells and the remodeling of extracellular matrix 12,14) while FGF-2 has a potent angiogenic effect 15) . IL-6 is one of the well-known and plentiful inflammatory cytokines 16) . In this regard, Koh et al 17) studied the cytokine production from osteoblastic cells in contact with MTA, and they found that MTA has an up-regulating effect on the expression of IL-1α , IL-1β , and IL-6 from osteoblasts.…”

Section: Department Of Conservative Dentistry College Of Dentistry mentioning

confidence: 99%

The effect of mineral trioxide aggregate on the production of growth factors and cytokine by human periodontal ligament fibroblasts

Kwon

Lim

Baek

et al. 2007

J Korean Acad Conserv Dent

View full text Add to dashboard Cite

Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative

Cited by 235 publications

References 27 publications

Indirect co-culture with tenocytes promotes proliferation and mRNA expression of tendon/ligament related genes in rat bone marrow mesenchymal stem cells

Indirect co-culture with tenocytes promotes proliferation and mRNA expression of tendon/ligament related genes in rat bone marrow mesenchymal stem cells

A Three-Dimensional Cell Culture Model to Study the Mechano-Biological Behavior in Periodontal Ligament Regeneration

The effect of mineral trioxide aggregate on the production of growth factors and cytokine by human periodontal ligament fibroblasts

Contact Info

Product

Resources

About