Autofocusing method for tilted image plane detection in digital holographic microscopy

Kostencka, Julianna; Kozacki, Tomasz; Liżewski, Kamil

doi:10.1016/j.optcom.2013.01.078

Cited by 36 publications

(11 citation statements)

References 31 publications

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“…Indeed, if digital holographic reconstruction can refocus a sample slice-by-slice as the focusing stage of a classical imaging system, the refocusing degree of an object has to be determined by an external criterion. Several different solutions have been developed [29][30][31], the one we use in this study is based on the invariance of both energy and amplitude [32,33]. Those invariance properties allow building two focus criteria, respectively for pure amplitude and pure phase objects, based on the score of the integrated amplitude modulus.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Caprio¹,

Mallahi²,

Ferraro³

et al. 2014

Biomed. Opt. Express

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In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

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Section: Theoretical Backgroundmentioning

confidence: 99%

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Caprio¹,

Mallahi²,

Ferraro³

et al. 2014

Biomed. Opt. Express

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“…In the case of the microlenses, the evaluation of the image sharpness was performed by calculating the normalized variance [27] of an amplitude distribution, which is a standard method for focus evaluation of pure phase objects. In the case of the USAF target, sharpness metrics based on calculation of the image gradient were applied, which is especially designed for amplitude objects [41]. As can be seen from Figs.…”

Section: B In-focus Plane Detection With a Standard Autofocusing Methodsmentioning

confidence: 99%

High-precision topography measurement through accurate in-focus plane detection with hybrid digital holographic microscope and white light interferometer module

et al. 2014

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High-precision topography measurement of micro-objects using interferometric and holographic techniques can be realized provided that the in-focus plane of an imaging system is very accurately determined. Therefore, in this paper we propose an accurate technique for in-focus plane determination, which is based on coherent and incoherent light. The proposed method consists of two major steps. First, a calibration of the imaging system with an amplitude object is performed with a common autofocusing method using coherent illumination, which allows for accurate localization of the in-focus plane position. In the second step, the position of the detected in-focus plane with respect to the imaging system is measured with white light interferometry. The obtained distance is used to accurately adjust a sample with the precision required for the measurement. The experimental validation of the proposed method is given for measurement of high-numerical-aperture microlenses with subwavelength accuracy.

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“…In Ref. 74,Kostencka et al proposed an appropriate tool for automatic localization of a tilted optimum focus plane. The method is based on the estimation of the focusing condition of the optical field by evaluating the sharpness in its amplitude distribution.…”

Section: Diffraction Between Arbitrarily Oriented Planesmentioning

confidence: 99%

Extended focus imaging in digital holographic microscopy: a review

2014

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The microscope is one of the most useful tools for exploring and measuring the microscopic world. However, it has some restrictions in its applications because the microscope's depth of field (DOF) is not sufficient for obtaining a single image with the necessary magnification in which the whole longitudinal object volume is in focus. Currently, the answer to this issue is the extended focused image. Techniques proposed over the years to overcome the limited DOF constraint of the holographic systems and to obtain a completely in-focus image are discussed. We divide them in two macro categories: the first one involves methods used to reconstruct three-dimensional generic objects (including techniques inherited from traditional microscopy, such as the sectioning and merging approach, or multiplane imaging), while the second area involves methods for objects recorded on a tilted plane with respect to hologram one (including not only the use of reconstruction techniques and rotation matrices, but also the introduction of a numerical cubic phase plate or hologram deformations). The aim is to compare these methods and to show how they work under the same conditions, proposing different applications for each. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Autofocusing method for tilted image plane detection in digital holographic microscopy

Cited by 36 publications

References 31 publications

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

High-precision topography measurement through accurate in-focus plane detection with hybrid digital holographic microscope and white light interferometer module

Extended focus imaging in digital holographic microscopy: a review

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