Autologous Bone Marrow Aspirate Therapy in Wound Healing

Chittoria, Ravi Kumar; Nandhagopal, Vijayaraghavan; Mohapatra, Devi Prasad; Thiruvoth, Friji Meethale; Sivakumar, Dinesh Kumar; Asokan, Arjun

doi:10.1089/wound.2014.0612

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Zhou et al showed that a combination of AD‐MSCs and their ECM increases wound healing 74 . PRP is a rich source of cytokines and growth factors important for wound healing, including EGF, bFGF, HGF, PDGF, TGF‐β1 and VEGF.…”

Section: Products From Peripheral Blood – Prp and Prfmentioning

confidence: 99%

“…46 Zhou et al showed that a combination of AD-MSCs and their ECM increases wound healing. 74 PRP is a rich source of cytokines and growth factors important for wound healing, including EGF, bFGF, HGF, PDGF, TGF-β1 and VEGF. Recent studies have shown that PRP has an anti-inflammatory effect and regulates macrophages to increase wound healing.…”

Section: Association Between Orthobiologicsmentioning

confidence: 99%

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The role of orthobiologics in chronic wound healing

Domingues,

von Rautenfeld,

Kavalco

et al. 2024

International Wound Journal

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Chronic wounds, characterized by prolonged healing processes, pose a significant medical challenge with multifaceted aetiologies, including local and systemic factors. Here, it explores the complex pathogenesis of chronic wounds, emphasizing the disruption in the normal phases of wound healing, particularly the inflammatory phase, leading to an imbalance in extracellular matrix (ECM) dynamics and persistent inflammation. Senescent cell populations further contribute to impaired wound healing in chronic lesions. Traditional medical management focuses on addressing underlying causes, but many chronic wounds resist to conventional treatments, necessitating innovative approaches. Recent attention has turned to autologous orthobiologics, such as platelet‐rich plasma (PRP), platelet‐rich fibrin (PRF) and mesenchymal stem cells (MSCs), as potential regenerative interventions. These biologically derived materials, including bone marrow aspirate/concentrate (BMA/BMAC) and adipose tissue‐derived stem cells (ADSCs), exhibit promising cytokine content and regenerative potential. MSCs, in particular, have emerged as key players in wound healing, influencing inflammation and promoting tissue regeneration. This paper reviews relevant scientific literature regarding basic science and brings real‐world evidence regarding the use of orthobiologics in the treatment of chronic wounds, irrespective of aetiology. The discussion highlights the regenerative properties of PRP, PRF, BMA, BMAC and SVF, showcasing their potential to enhance wound healing. Despite advancements, further research is essential to elucidate the specific roles of each orthobiologic and determine optimal applications for different wound types. The conclusion underscores the evolving landscape in chronic wound management, with a call for more comprehensive studies to refine treatment strategies and maximize the benefits of regenerative medicine.

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Section: Products From Peripheral Blood – Prp and Prfmentioning

confidence: 99%

Section: Association Between Orthobiologicsmentioning

confidence: 99%

The role of orthobiologics in chronic wound healing

Domingues,

von Rautenfeld,

Kavalco

et al. 2024

International Wound Journal

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“…Поэтому альтернативой могут стать низкозатратные и доступные для базового звена системы здравоохранения методы стимуляции раневого заживления аутологичными аспиратами красного костного мозга [5][6][7]. Проведено проспективное простое контролируемое рандомизированное клиническое исследование течения 2-й фазы раневого процесса у 72 пациентов с гнойными ранами различного происхождения и локализации в период с 2017 по 2021 г. Рандомизацию осуществляли методом игральных костей.…”

Section: Introductionunclassified

Влияние Аутологичных Аспиратов Красного Костного Мозга На Раневой Процесс

Федянин¹,

Косинец²,

Коваленко³

et al. 2021

Хирургия. Восточная Европа

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Цель. Исследовать влияние аутологичных аспиратов красного костного мозга на раневой процесс. Материалы и методы. Выполнено рандомизированное исследование по изучению влияния аутомиелоаспиратов на раневой процесс у 72 пациентов с хирургическими инфекциями кожи и мягких тканей. Пациенты были разделены на основную и контрольную группы. В основной группе для стимуляции раневого заживления применялись аутологичные аспираты красного костного мозга. Стимуляция выполнялась при наличии признаков хронизации раневого процесса. Планиметрию производили с помощью метода А.Н. Лызикова и соавторов (2008) и программного обеспечения Adobe Photoshop (Adobe Systems, США). Для оценки клеточной картины приготовление цитологических отпечатков выполняли с применением метода М.П. Покровской и М.С. Макарова (1942). Скорость раневого заживления рассчитывали по формуле Л.Н. Поповой. Кислотность раневого отделяемого определяли с помощью стерильной универсальной индикаторной бумаги. Иммуногистохимическое окрашивание гистологического материала выполнялось с использованием моноклональных антител anti-Сollagen Type 1 и BondТМ Ki67. Для морфометрической оценки экспресии маркера в образцах применяли программное обеспечение ImageJ 1.45s. Результаты. Скорость заживления ран в основной группе составила 6 [5; 35]% в день, а в контрольной группе - 3 [3; 28]% в день и в 2 раза была достоверно выше при применении аутологичных аспиратов красного костного мозга (р<0,001). Сроки подготовки ран к пластическому закрытию в основной группе составили 5 [3; 7] дней, а в контрольной - 12 [9; 15] дней (р<0,001). Выявлено достоверное увеличение интенсивности экспрессии маркеров COL1 и Ki67 при применении аутомиелоаспиратов. Выводы. Аутологичные аспираты красного костного мозга являются мощными стимуляторами раневого заживления. Purpose. To study the effect of autologous red bone marrow aspirates on the wound healing process. Materials and methods. A randomized study was carried out to study the effect of automyeloaspirates on the wound healing process in 72 patients with surgical infections of the skin and soft tissues. Patients were divided into main and control groups. In the main group autologous red bone marrow aspirates were used to stimulate wound healing. Stimulation was performed in case of chronic wound healing signs. For planimetry the method of A.N. Lyzikov et al. (2008) and Adobe Photoshop software (Adobe Systems, USA) was used. To estimate the cellular pattern the cytological specimens were prepared with the M.P. Pokrovskaya and M.S. Makarov method (1942). The rate of wound healing was calculated using the formula of L.N. Popova. To determine the acidity of the wound discharge sterile the universal indicator paper was used. Immunohistochemical staining of histological material was performed using anti-Collagen Type 1 and BondTM Ki67 monoclonal antibodies. For morphometric assessment of the samples markers expression the ImageJ 1.45s software was applied. Results. The rate of wound healing in the main group was 6 [5; 35]% per day, and in the control group - 3 [3; 28]% per day and 2 times was significantly higher comparing with autologous red bone marrow aspirates (p<0,001). The duration of the wounds preparation for surgical plastic in the main group was 5 [3; 7] days, and in the control - 12 [9; 15] (p<0,001). A significant increase in the COL1 and Ki67 markers expression intensity was revealed in case of automyeloaspirates the use. Conclusions. Thus, autologous bone marrow aspirates are potent stimulators of wound healing.

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Autologous bone marrow aspirates in the treatment of extensive purulent wounds

Fedzianin

2020

Bulletin of Pirogov National Medical &Amp; Surgical Center

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Autologous Bone Marrow Aspirate Therapy in Wound Healing

Cited by 5 publications

References 9 publications

The role of orthobiologics in chronic wound healing

The role of orthobiologics in chronic wound healing

Влияние Аутологичных Аспиратов Красного Костного Мозга На Раневой Процесс

Autologous bone marrow aspirates in the treatment of extensive purulent wounds

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