Autologous fibrin sealant in reconstructive rabbit oviduct microsurgery

Weis-Fogh, Ulla Sivertsen; Schroeder, Erik; Olesen, H. P.; Sørensen, Steen

doi:10.1016/s0015-0282(16)53653-x

Cited by 6 publications

(13 citation statements)

References 7 publications

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“…After a postoperative recovery of 4 weeks, the rate of pregnancy was the same in animals where the reanastomosis was per formed with the use of fibrin adhesive, autol ogous and heterologous, as in animals with PGA-sutured anastomosis and in nonoperated animals [3]. When the rabbits were killed.…”

Section: Discussionmentioning

confidence: 98%

“…Our previous experiments [1][2][3] have shown the technical advantages (such as re duced bleeding, reduced operation time and a significant lower adhesion formation) of using fibrin adhesive in reanastomosis of the rabbit oviduct compared to the use of conventional suturing. After a postoperative recovery of 4 weeks, the rate of pregnancy was the same in animals where the reanastomosis was per formed with the use of fibrin adhesive, autol ogous and heterologous, as in animals with PGA-sutured anastomosis and in nonoperated animals [3].…”

Section: Discussionmentioning

confidence: 99%

“…The blood was taken from the rabbit immediately before surgery. The method was the same as described previously [3,4], Plasma was separated from the blood by centrifugation. The fibrin ogen was precipitated from plasma with 1.5 M ethanol at 0°C, separated from the plasma by centrifugation and redissolved at 37 °C in 0.15 ml ethanol-free plas ma.…”

Section: Methodsmentioning

confidence: 99%

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Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Weis-Fogh

Pedersen²,

Schroeder³

et al. 1993

Eur Surg Res

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The morphology of the healing process of microsurgical re-anastomosis of the rabbit oviduct with the use of fibrin adhesive, autologous and heterologous, and conventional sutures is described. Both oviducts in 48 rabbits were cut and reanastomoses were performed. The rabbits were killed at different intervals after the operations, ranging from 2 h to 28 days, and the anastomoses were evaluated by histomorphological examination.The autologous fibrin adhesive was absorbed after a week and an uncomplicated healing was observed. Heterologous fibrin adhesive caused a granulomatous inflammation interpreted as an immune reaction of the host to the foreign protein, and conventional suturing resulted in severe tissue damage with an intensive inflammatory reaction.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Weis-Fogh

Pedersen²,

Schroeder³

et al. 1993

Eur Surg Res

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“…A patency rate of 75% led to the conclusion that the microsurgical anastomosis using fine microsutures was the most effective method of oviduct reconstruction. However, Gauwerky et al (1989) and Weis-Fogh et al (1990) demonstrated that the gluing was as effective as microsuturing although they did not study the histological repair. The CO2 laser was applied successfully in the anastomosis of the uterine horn in rabbits by Klink et al (1983).…”

Section: Discussionmentioning

confidence: 99%

“…Trials with the laser (Lyon et al 1987) and tissue adhesives (Weis-Fogh et al, 1990) show that neither is superior to sutures.…”

Section: Introductionmentioning

confidence: 99%

Study of two simplified microsurgical techniques for uterine horn anastomosis in rat

Caballero-Gómez

Ortega-Moreno

1993

Arch Gynecol Obstet

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This study compares two simplified techniques, which use only 2 and 4 sutures respectively, with a conventional technique with 8 sutures for fallopian tube anastomosis. Experimentally these techniques were performed on the uterine horns of fifteen female rats. A 100% patency rate was obtained with all three techniques. No difference in the mucosal, muscular and serosal regeneration was observed in the three groups at 10, 20 and 60 days. Two months after surgery, the serosa, muscularis and mucosal layers were completely continuous in all groups. The operating with 2 sutures (5'30" +/- 1'10") was significantly less than with 4 (9'09" +/- 0'55", P < 0.05 ANOVA) and 8 sutures (15'12" +/- 1'41", P < 0.05 ANOVA). A minimum inflammatory reaction to sutures was observed in all three groups at 60 days after surgery. The results suggest that with 2 sutures are all that is needed for tissue repair.

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Fibrin Glue Vasal Anastomosis Compared To Conventional Sutured Vasovasostomy in the Rat

Silverstein

Mellinger

1991

Journal of Urology

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Vasectomy reversal has become a frequently performed surgical procedure with best results obtained with the use of the operating microscope and microsurgical technique. The present study was undertaken to evaluate the use of fibrin glue ("Tisseel", Immuno U.S., Inc.) for vasovasostomy and to compare this technique to conventional sutured vasovasostomy. Utilizing 60 male Sprague Dawley rats, a conventional two layered sutured anastomosis of vasovasostomy (30 rats) was compared to a fibrin glue technique of vasal anastomosis (30 rats). The fibrin glue technique was performed with two transmural sutures, was unstented, and utilized the biological glue to seal the anastomosis. The contralateral vas of each animal underwent vasectomy and reapproximation of unligated ends so that the rate of spontaneous recanalization could be accessed. Rats were sacrificed at 24 hours, one week, four weeks, and three months postvasovasostomy. The vasal specimens were evaluated for gross patency, presence and size of sperm granuloma, mean flow rates at varying infusion pressures, tensile strength measurements and histologic studies. Combining the one and three month groups, a similar patency rate was obtained by either technique; 83% (n = 18) for the sutured group, and 90% (n = 21) for the fibrin glue group. The rate of spontaneous recanalization of the contralateral vasa in the one and three month animals was 8% (n = 38). The mean flow rates obtained at high and low infusion pressures were not statistically different for the two techniques. The tensile strength of the glue anastomosis averaged 78% of the tensile strength achieved by the conventional sutured technique. The incidence of sperm granuloma after vasovasostomy was 28% for the fibrin glue group and 61% for the sutured group. Additionally, 67% of granulomas were small (less than 3 mm.) in the glue group, compared to only 36% in the sutured group. Histology revealed similar morphological changes in the area of anastomosis with either technique. Operative time for sutured vasovasostomy averaged 24 minutes, compared to an average of 11 minutes for the glue assisted vasovasostomy. The use of fibrin glue allowed the performance of a sperm tight patent anastomosis that had the advantages of reduced incidence of sperm granuloma formation, reduced operative time, and less microsurgical skill required to perform the anastomosis.

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Autologous fibrin sealant in reconstructive rabbit oviduct microsurgery

Cited by 6 publications

References 7 publications

Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Study of two simplified microsurgical techniques for uterine horn anastomosis in rat

Fibrin Glue Vasal Anastomosis Compared To Conventional Sutured Vasovasostomy in the Rat

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