Autologously transplanted dermal fibroblasts improved diabetic wound in rat model

Mutlu, Hasan Serdar; Erdoğan, Aslı; Tapul, Leyla

doi:10.1016/j.acthis.2020.151552

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…10 days after the STZ administration, wound formation studies were performed. In order to begin the experiments, rats were kept for 10 days to obtain a chronic hyperglycemic state [43][44][45].…”

Section: Experimental Design Of Wound Healing Experimentsmentioning

confidence: 99%

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Çam

Ertaş

Alenezi

et al. 2021

Materials Science and Engineering: C

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The combination of oral antidiabetic drugs, pioglitazone, metformin, and glibenclamide, which also exhibit the strongest anti-inflammatory action among oral antidiabetic drugs, were loaded into chitosan/gelatin/polycaprolactone (PCL) by electrospinning and polyvinyl pyrrolidone (PVP)/PCL composite nanofibrous scaffolds by pressurized gyration to compare the diabetic wound healing effect. The combination therapies significantly accelerated diabetic wound healing in type-1 diabetic rats and organized densely packed collagen fibers in the dermis, it also showed better regeneration of the dermis and epidermis than single drug-loaded scaffolds with less inflammatory cell infiltration and edema. The formation of the hair follicles started in 14 days only in the combination therapy and lower proinflammatory cytokine levels were observed compared to single drug-loaded treatment groups. The combination therapy increased the wettability and hydrophilicity of scaffolds, demonstrated sustained drug release over 14 days, has high tensile strength and suitable cytocompatibility on L929 (mouse fibroblast) cell and created a suitable area for the proliferation of fibroblast cells. Consequently, the application of metformin and pioglitazone-loaded chitosan/gelatin/PCL nanofibrous scaffolds to a diabetic wound area offer high bioavailability, fewer systemic side effects, and reduced frequency of dosage and amount of drug.

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Section: Experimental Design Of Wound Healing Experimentsmentioning

confidence: 99%

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Çam

Ertaş

Alenezi

et al. 2021

Materials Science and Engineering: C

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“…Previously, the tissue block was often cut into about 1 × 1mm 2 in size with neat and smooth edges (Proudfoot and Shanahan 2011; Voicekhovskaya et al 2012;Mutlu et al 2020). Nevertheless, we, after many pre-experiments, found that 2×2mm 2 size of tissue block was optimum because the too-small tissue block tended to shrink during the adherent process and be unfavorable to reuse, whereas the toolarge one kept the cells poor in nutrients and easy to die.…”

Section: Discussionmentioning

confidence: 99%

A Newly Improved Method of Primary Cell Culture: Tissue Block with Continuous Adhesion Subculture in Skin Fibroblast

Deng

Liu

Tang

et al. 2023

Preprint

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Background Fibroblasts (FBs) have been widely used as a typical in vitro cell model for investigating the biological processes and cell pathophysiological mechanisms. However, FBs are prone to senescence in cell culture process after several passages. Thus, a new approach to cell culture is quite required to enhance the viability of cells. Objective To explore a novel method of primary cell culture based on skin FBs. Methods Dermal tissue blocks were obtained from BALB/c neonatal mice and randomly divided into experimental group and control group. The experimental group received the newly improved culture method, namely, continuous adherence subculture of tissue block (CASTB) method; while the traditional subculture method was applied in the control group. Cells at 1st, 5th and 10th passages were collected and identified by using histological/immunohistochemical and western blot analysis. Cellular viability, proliferation, senescence and apoptosis were analyzed through application of cell growth curve, CCK-8 assay, Ki67 assay, β-galactosidase staining, flow cytometry and western blot analysis. Results Cells under two culture patterns showed vimentin positive expression via immunohistochemistry and western blot assay. With the increase of passage times, the cellular growth rate in the control group gradually decreased, but no alterations emerged from the experimental group. CASTB remarkably promoted cell growth and proliferation. Besides, a lower apoptosis tendency emerged from the experimental group than the control goup with the increasing passages. Conclusion The method of CASTB may offer a large number of primary FBs with higher efficiency and success rate, which is worth of further popularization and application.

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“…The formation of granulation tissue, the promotion of angiogenesis and epithelization are important treatment methods for chronic wound healing ( 101 ). In addition, new research shows that cells obtained in dermal connective tissue can be used as mesenchymal stem cells, because the reduction and delay of FGF-7 growth factor production in diabetic patients improves with the improvement of this cell transplantation, and contribute to wound healing ( 102 ). FGF-7 is damaged in diabetic skin wounds, but the specific regeneration mechanism remains to be proved.…”

Section: Fgf-7 and Diabetic Wound Healingmentioning

confidence: 99%

Fibroblast Growth Factor in Diabetic Foot Ulcer: Progress and Therapeutic Prospects

et al. 2021

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Diabetic foot ulcer (DFU) is a combination of neuropathy and various degrees of peripheral vasculopathy in diabetic patients resulting in lower extremity infection, ulcer formation, and deep-tissue necrosis. The difficulty of wound healing in diabetic patients is caused by a high glucose environment and various biological factors in the patient. The patients’ skin local microenvironment changes and immune chemotactic response dysfunction. Wounds are easy to be damaged and ulcerated repeatedly, but difficult to heal, and eventually develop into chronic ulcers. DFU is a complex biological process in which many cells interact with each other. A variety of growth factors released from wounds are necessary for coordination and promotion of healing. Fibroblast growth factor (FGF) is a family of cell signaling proteins, which can mediate various processes such as angiogenesis, wound healing, metabolic regulation and embryonic development through its specific receptors. FGF can stimulate angiogenesis and proliferation of fibroblasts, and it is a powerful angiogenesis factor. Twenty-three subtypes have been identified and divided into seven subfamilies. Traditional treatments for DFU can only remove necrotic tissue, delay disease progression, and have a limited ability to repair wounds. In recent years, with the increasing understanding of the function of FGF, more and more researchers have been applying FGF-1, FGF-2, FGF-4, FGF-7, FGF-21 and FGF-23 topically to DFU with good therapeutic effects. This review elaborates on the recently developed FGF family members, outlining their mechanisms of action, and describing their potential therapeutics in DFU.

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Autologously transplanted dermal fibroblasts improved diabetic wound in rat model

Cited by 6 publications

References 41 publications

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

A Newly Improved Method of Primary Cell Culture: Tissue Block with Continuous Adhesion Subculture in Skin Fibroblast

Fibroblast Growth Factor in Diabetic Foot Ulcer: Progress and Therapeutic Prospects

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