Autolysis of high-GC isolates of Pseudomonas putrefaciens

Levin, Robert E.; Sickle, Camilla Van

doi:10.1007/bf00399459

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…The group with a relatively low G C content tends to be weighted with strains of non-clinical origin, and a number of distinctive phenotypic characters (e.g. failure to grow in the presence of 6 to 776 NaC1) have been reported (Levin, 1972;Riley et al, 1972;Holmes et al, 1975;Williams & Levin, 1975;Levin & Van Sickle, 1976). In a recent numerical taxonomic study, Lee et al (1977) were unable to correlate the GC content of a strain with its salt tolerance or other properties, but proposed that P. putrefaciens (their phenon E) be transferred to the genus Alteromonas as Alteromonas putrefaciens, in accord with an earlier suggestion (Gavini & Leclerc, 1974).…”

mentioning

confidence: 99%

Lipid Composition and Chemotaxonomy of Pseudomonas putrefaciens (Alteromonas putrefaciens)

Wilkinson¹,

Caudwell²

1980

Microbiology

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The major polar lipids in cells of Pseudomonas putrefaciens NCIB 10472 grown on nutrient agar were phosphatidylethanolamine, phosphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. An additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. The wall lipids from broth cultures of three further strains of P. putrefaciens (NCIB 10471, NCIB 11 156 and NCTC 10737) contained all of the above lipids, and in two cases (strains NCIB 10471 and NCIB 11 156) had an unusually high content of free fatty acid. Fatty acid compositions of the extractable lipids were qualitatively similar for all four strains : the major components were iso-pentadecanoic acid, pentadecanoic acid, a cis-heptadecenoic acid and a cis-hexadecenoic acid. Anteiso fatty acids were minor components in strain NCIB 10472. Lipid mixtures in which the ornithine amide lipid was present also contained small amounts of P-hydroxy fatty acids: in strain NCIB 10472 the major ones were the straight-chain and iso-branched C,, acids. Lipopolysaccharides from all four strains had similar, complex fatty acid compositions. The major non-hydroxy acids were the straight-chain and iso-branched C,, acids. P-Hydroxy acids common to all strains included the straight-chain Cll, C12, CI3, C14 and C15 acids, together with branched-chain C13 and C15 acids probably belonging to the is0 series. The lipopolysaccharide from strain NCIB 10472 also contained C12 and C,, hydroxy acids of the same series, and small amounts of C13 and CI5 /I-hydroxy acids probably belonging to the anteiso series. The close resemblance in both polar lipid and fatty acid compositions between strains of P. putrefaciens and Pseudomonas rubeseens is further evidence that these species are synonymous. Significant differences between the lipids and fatty acids of P. putrefaciens and those reported for a strain of Alteromonas haloplanktis do not harmonize with a proposal to transfer the former organism to the genus Alteromonas.

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confidence: 99%

Lipid Composition and Chemotaxonomy of Pseudomonas putrefaciens (Alteromonas putrefaciens)

Wilkinson¹,

Caudwell²

1980

Microbiology

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“…Cultures were grown in 50-ml portions of TSB in 250-ml flasks with rotary agitation (200 rpm) at 20°C. The viscosity of broth cultures was determined as previously described (11), with a capillary viscometer (model no. 200; Cannon-Fenske; Fisher Scientific Co., Boston) at 20°C in a temperature-controlled water bath.…”

Section: Methodsmentioning

confidence: 99%

Relative Incidence of Alteromonas putrefaciens and Pseudomonas putrefaciens in Ground Beef

Parker

Levin

1983

Appl Environ Microbiol

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“…The local density of SSTRs was correlated with the G+C content in the first step, since the G+C content was well-known to be associated with several functional features [20][21][22][23][24].…”

Section: Introduction Strs/sstrs and K-mer Analysismentioning

confidence: 99%

Specific Patterns in Correlations of Super-Short Tandem Repeats (SSTRs) with G+C Content, Genic and Intergenic Regions, and Retrotransposons on All Human Chromosomes

Henn,

Sievers,

Hausmann

et al. 2023

Genes

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The specific characteristics of k-mer words (2 ≤ k ≤ 11) regarding genomic distribution and evolutionary conservation were recently found. Among them are, in high abundance, words with a tandem repeat structure (repeat unit length of 1 bp to 3 bp). Furthermore, there seems to be a class of extremely short tandem repeats (≤12 bp), so far overlooked, that are non-random-distributed and, therefore, may play a crucial role in the functioning of the genome. In the following article, the positional distributions of these motifs we call super-short tandem repeats (SSTRs) were compared to other functional elements, like genes and retrotransposons. We found length- and sequence-dependent correlations between the local SSTR density and G+C content, and also between the density of SSTRs and genes, as well as correlations with retrotransposon density. In addition to many general interesting relations, we found that SINE Alu has a strong influence on the local SSTR density. Moreover, the observed connection of SSTR patterns to pseudogenes and -exons might imply a special role of SSTRs in gene expression. In summary, our findings support the idea of a special role and the functional relevance of SSTRs in the genome.

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Autolysis of high-GC isolates of Pseudomonas putrefaciens

Cited by 8 publications

References 13 publications

Lipid Composition and Chemotaxonomy of Pseudomonas putrefaciens (Alteromonas putrefaciens)

Lipid Composition and Chemotaxonomy of Pseudomonas putrefaciens (Alteromonas putrefaciens)

Relative Incidence of Alteromonas putrefaciens and Pseudomonas putrefaciens in Ground Beef

Specific Patterns in Correlations of Super-Short Tandem Repeats (SSTRs) with G+C Content, Genic and Intergenic Regions, and Retrotransposons on All Human Chromosomes

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