Automated analysis of antidepressants’ effect in the forced swim test

Куликов, А. В.; Морозова, М. В.; Kulikov, V. A.; Kirichuk, V. S.; Попова, Н. К.

doi:10.1016/j.jneumeth.2010.06.002

Cited by 32 publications

(9 citation statements)

References 29 publications

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“…With an integrated threshold algorithm, the threshold for immobility was fine-tuned (at a value of 20) prior to the actual analyses so that the mouse is considered immobile (minimal movements) when it is visually verified as such. This and other automated FST behavioral quantitation have been tested to serve as reliable methods that address the limitations of manual (visual) scoring by experimenters (26, 28). …”

Section: Methodsmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Val66Met Allele Impairs Basal and Ketamine-Stimulated Synaptogenesis in Prefrontal Cortex

Liu

Lee

et al. 2012

Biological Psychiatry

359

306

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Background Knock-in mice with the common human BDNF Val66Met polymorphism have impaired trafficking of BDNF mRNA to dendrites. Given evidence that local synapse formation is dependent on dendritic translation of BDNF mRNA, it was hypothesized that loss-of-function Met allele mice would show synaptic deficits both at baseline and in response to ketamine, an NMDA antagonist that stimulates synaptogenesis in prefrontal cortex (PFC). Methods Whole-cell recordings from layer V medial PFC pyramidal cells in brain slices were combined with 2-photon laser scanning for analysis of WT, Val/Met, and Met/Met mice both at baseline and in response to a low dose of ketamine. Results Val/Met and Met/Met mice were found to have constitutive atrophy of distal apical dendrites and decrements in apically-targeted excitatory postsynaptic currents (EPSCs) in layer V pyramidal cells of PFC. In addition, spine density and diameter were decreased, indicative of impaired synaptic formation/maturation (synaptogenesis). In Met/Met mice the synaptogenic effect of ketamine was markedly impaired, consistent with the idea that synaptogenesis is dependent on dendritic translation/release of BDNF. In parallel behavioral studies we found that the antidepressant response to ketamine in the forced swim test was blocked in Met/Met mice. Conclusions The results demonstrate that expression of the BDNF Met allele in mice results in basal synaptic deficits and blocks synaptogenic and antidepressant actions of ketamine in PFC, suggesting that the therapeutic response to this drug may be attenuated or blocked in depressed patients who carry the loss of function Met allele.

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Section: Methodsmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Val66Met Allele Impairs Basal and Ketamine-Stimulated Synaptogenesis in Prefrontal Cortex

Liu

Lee

et al. 2012

Biological Psychiatry

359

306

View full text Add to dashboard Cite

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“…Fortunately, these methods do lend themselves to automation based on video analysis. Automated detection of the rodents in the video frame followed by measurements of the changes in various features of the object between frames has been tried and published before ( Hédou et al, 2001 ; Kurtuncu et al, 2005 ; Rocha et al, 2005 ; Cryns et al, 2007 ; Juszczak et al, 2008 ; Yuman et al, 2008 ; Kulikov et al, 2010 ; Hayashi et al, 2011 ; Gao et al, 2014 ; Pennington et al, 2019 ; Sturman et al, 2020 ). TST has also been automated using a strain-gauge apparatus ( Steru et al, 1987 ; Crowley et al, 2004 ; Mombereau et al, 2004 ; Strekalova et al, 2004 ; Cryan et al, 2005 ; Alexandre et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

DBscorer: An Open-Source Software for Automated Accurate Analysis of Rodent Behavior in Forced Swim Test and Tail Suspension Test

Nandi

Virmani

Barve

et al. 2021

eNeuro

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“…Other behavioral tests were held between 15:00 and 18:00 in the dark time. The EthoStudio software was applied for automatic tracking of mouse behavior in OF, EPM and FS tests (Kulikov et al, 2008(Kulikov et al, , 2010(Kulikov et al, , 2014. In OF and FS tests we used the transmitted (inverted) light for automatic tracking of mouse behavior when the light was transmitted through the arena to a WEB camera placed at 80 cm above the arena and connected to a computer via a USB 3.0 port.…”

Section: генетика животных / Animal Geneticsmentioning

confidence: 99%

“…Mice were placed for 6 min into a clear plastic tank (18 cm in diameter and 30 cm in height) filled with water at temperature 25 °C for 2/3 of the volume (20 cm). The water tank was placed on a semitransparent platform and illuminated (150 lx) with one halogen lamp (35 W) placed 40 cm below the platform (Kulikov et al, 2010). Behavior in the FS test was automatically evaluated for the last four minutes of the test using EthoStudio software by immobility time (s) and rate of alteration of the animal silhouette (%).…”

Section: генетика животных / Animal Geneticsmentioning

confidence: 99%

Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior

et al. 2019

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Decrease in natural illumination in fall/winter months causes depressive-like seasonal affective disorders in vulnerable individuals. Obesity is another risk factor of depression. The lethal yellow (A Y ) mutation causes ectopic expression of agouti protein in the brain. Mice heterozygous for A Y mutation (A Y /a) are obese compared to their wild-type littermates (a/a). The main aims of the study were to investigate the effects of A Y mutation, photoperiod and the interaction between these factors on daily activity dynamics, feeding, locomotor and exploratory activities, anxiety-related and depressive-like behaviors in mild stress condition. Six weeks old mouse males of A Y /a and a/a lines were divided into four groups eight animals each and exposed to long-(14 h light and 10 h darkness) or short-(4 h light and 20 h darkness) day conditions for 28 days. Then the behavior of these mice was successively investigated in the home cage, open field, elevated plus-maze and forced swim tests. We did not observed any effect of A Y mutation on the general activity, water and food consumption in the home cage; locomotion and exploration in the open field test; anxiety-related behavior in the open field and elevated plus-maze tests. At the same time, A Y mutation increased depressive-like immobility time in the forced swim test (F 1.28 = 20.03, p = 0.00012). Shortday conditions decreased nocturnal activity in the home cage, as well as locomotion (F 1.28 = 16.33, p = 0.0004) and exploration (F 1.28 = 16.24, p < 0.0004) in the open field test. Moreover, short-day exposition decreased time spent in the center of the open field (F 1.28 = 6.57, p = 0.016) and in the open arms of the elevated plus-maze (F 1.28 = 12.08, p = 0.0017) tests and increased immobility time in the forced swim test (F 1.28 = 9.95, p = 0.0038). However, no effect of the interaction between A Y mutation and photoperiod on immobility time in the forced swim test was observed. Therefore, short-day photoperiod and A Y mutation increased depressive-like behavior in the forced swim test by means of different mechanisms.For citation: Bazhenova E.Y., Fursenko D.V., Khotskin N.V., Sorokin I.E., Kulikov A.V. Effect of lethal yellow (A Y ) mutation and photoperiod alterations on mouse behavior.

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Automated analysis of antidepressants’ effect in the forced swim test

Cited by 32 publications

References 29 publications

Brain-Derived Neurotrophic Factor Val66Met Allele Impairs Basal and Ketamine-Stimulated Synaptogenesis in Prefrontal Cortex

Brain-Derived Neurotrophic Factor Val66Met Allele Impairs Basal and Ketamine-Stimulated Synaptogenesis in Prefrontal Cortex

DBscorer: An Open-Source Software for Automated Accurate Analysis of Rodent Behavior in Forced Swim Test and Tail Suspension Test

Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior

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