Automated microfluidically controlled electrochemical biosensor for the rapid and highly sensitive detection of Francisella tularensis

Dulay, Samuel; Gransee, Rainer; Julich, Sandra; Tomaso, Herbert; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2014.03.024

Cited by 22 publications

(9 citation statements)

References 25 publications

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“…To circumvent these problems substantial effort has been put into developing site-directed antibody immobilization methods (Makaraviciute and Ramanaviciene 2013). Immobilization of antibody fragments has been one of the most widely used in site-directed coupling techniques applied in biosensor development (Dulay et al 2014, Wu et al 2014, nanostructural modification (Hu et al 2010, Quarta et al 2015 and labeling applications (Pereira andLai 2008, Cho et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Considerations in producing preferentially reduced half-antibody fragments

Makaraviciute

Jackson

Millner

et al. 2016

Journal of Immunological Methods

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Half-antibody fragments are a promising reagent for biosensing, drug-delivery and labeling applications, since exposure of the free thiol group in the Fc hinge region allows oriented Preferential reduction of rabbit anti-myoglobin IgG antibodies was optimized and the highest half-antibody yield was obtained with 35 mM TCEP. Finally, it has been demonstrated that produced anti-myoglobin half-IgG fragments retained their binding activity.

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Section: Introductionmentioning

confidence: 99%

Considerations in producing preferentially reduced half-antibody fragments

Makaraviciute

Jackson

Millner

et al. 2016

Journal of Immunological Methods

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“…Automated sensing is optimal for use in a biothreat situation, as its simplicity results in ease of training in regard to large-scale testing. Work done by Dulay et al 88 resulted in the creation of a fully automated sensing platform using gold electrodes and microfluidics to assist in automation. The only hands-on time is the addition of sample, with a complete run time of <20 min.…”

Section: Tularemiamentioning

confidence: 99%

“…The setup contains a peristaltic pump positioned behind the reservoirs to provide solution flow into the electrode array mounted within the microfluidics. a Electrode array with microfluidics placed in the platform and connected to the potentiostat for amperometric measurement; b a sample script-based assay program; c electrode array integrated with microfluidics; d a full front view of the prepared testing device; and e lithographically produced gold electrode array with internal reference electrodes and counter electrodes88…”

mentioning

confidence: 99%

The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

2021

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During the past year, disease has shown us the iron grip it can hold over a population of people. Health systems can be overwhelmed, economies can be brought into recession, and many people can be harmed or killed. When weaponized, diseases can be manipulated to create a detriment to health while becoming an economic burden on any society. It is consequently prudent that easy detection of bioweapons is available to governments for protecting their people. Electrochemical sensing displays many distinct advantages, such as its low limit of detection, low cost to run, rapid generation of results, and in many instances portability. We therefore present a wide array of electrochemical sensing platforms currently being fabricated, a brief summary of Class A bioweapons, and the potential future of bioweapon detection and biosafety.

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“…In addition to faster medical diagnostics [ 22 , 23 , 24 , 25 ], microfluidics is being applied in drugs of abuse testing [ 26 , 27 , 28 , 29 ], pollutant detection [ 30 , 31 , 32 , 33 , 34 ], combatting biowarfare [ 35 , 36 , 37 , 38 ] and also in laboratory routines in a research context, as described below.…”

Section: Microfluidics—from Small Benchtop Biosensors To High-thromentioning

confidence: 99%

Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering

Perestrelo

Águas

Rainer

et al. 2015

Sensors

134

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Recent advances in biomedical technologies are mostly related to the convergence of biology with microengineering. For instance, microfluidic devices are now commonly found in most research centers, clinics and hospitals, contributing to more accurate studies and therapies as powerful tools for drug delivery, monitoring of specific analytes, and medical diagnostics. Most remarkably, integration of cellularized constructs within microengineered platforms has enabled the recapitulation of the physiological and pathological conditions of complex tissues and organs. The so-called “organ-on-a-chip” technology, which represents a new avenue in the field of advanced in vitro models, with the potential to revolutionize current approaches to drug screening and toxicology studies. This review aims to highlight recent advances of microfluidic-based devices towards a body-on-a-chip concept, exploring their technology and broad applications in the biomedical field.

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Automated microfluidically controlled electrochemical biosensor for the rapid and highly sensitive detection of Francisella tularensis

Cited by 22 publications

References 25 publications

Considerations in producing preferentially reduced half-antibody fragments

Considerations in producing preferentially reduced half-antibody fragments

The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering

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