Automated Synthesis of Peptides

Abstract

essary for self-sufficiency will certainly prevent the attainment of this goal within the foreseeable future. Still, it remains a goal toward which we are moving.Meanwhile, we may expect to see the development of a number of selfsufficient regional calibration centers. With such centers in existence, the national standards laboratories may no longer need to make many calibrations; they can then concentrate more on the development and dissemination of procedures that will permit every major laboratory to reproduce accurately the standard for each quantity. essary for self-sufficiency will certainly prevent the attainment of this goal within the foreseeable future. Still, it remains a goal toward which we are moving.Meanwhile, we may expect to see the development of a number of selfsufficient regional calibration centers. With such centers in existence, the national standards laboratories may no longer need to make many calibrations; they can then concentrate more on the development and dissemination of procedures that will permit every major laboratory to reproduce accurately the standard for each quantity.References and Notes 1. Unfortunately the word standard has multiple meanings in English. In this article the word is used to mean a standard for physical measurement; that is to say, the physical realization of an agreed-upon unit (such as the meter or kilogram) for the expression of physical measurements.References and Notes 1. Unfortunately the word standard has multiple meanings in English. In this article the word is used to mean a standard for physical measurement; that is to say, the physical realization of an agreed-upon unit (such as the meter or kilogram) for the expression of physical measurements.

Production of anti-Helicobacter pylori urease-specific immunoglobulin in egg yolk using an antigenic epitope of H. pylori urease

“…Domains with a high degree of hydrophilicity were selected as peptide antigens. Two 15-residue peptides selected from amino acids 1-238 of UreA (UA-1, residues 18-32 and UA-2, residues 216-230) and three peptides of 15-16 residues selected from amino acids 1-569 of UreB (UB-1, residues 52-67; UB-2, residues 381-395; and UB-3, residues 396-410) were synthesized by the solid phase method of Merrifield (1965). In brief, the tert-butyloxycarbonyl-protected amino acids were obtained using a multipeptide synthesizer (Applied Biosystems).…”

Method for Correlation of Proton Magnetic Resonance Assignments in Different Solvents: Conformational Transition of Oxytocin and Lysine Vasopressin from Dimethylsulfoxide to Water

“…1) were prepared by the solid-phase method of peptide synthesis (11), and exhibited 450-500 U/mg of avian vasodepressor (12) and 270-300 U/mg of rat pressor (13) activities, respectively, which is in line with potencies previously described for these hormones (14). Stock solutions of each hormone (3% w/v) were prepared in both Me2SO and water, and were acidified with HCO (to pH 2.5 for oxytocin and to pH 4.2 for Lys-VP).…”

Comparative characteristic of the methods of protein antigens epitope mapping

“…Цей підхід базується на біоорганіч ному синтезі пептидів на основі спеціальної смоли-матриці [27]. Загальна стратегія методу адресо-вана до імітації конформаційного або лінійного епітопа шляхом використання синтетично-го пептиду, хімічнозв'язаного із полімерним носієм.…”