Automatic fabrication of 3-dimensional tissues using cell sheet manipulator technique

Kikuchi, Tetsutaro; Shimizu, Tatsuya; Wada, Masaaki; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2013.12.014

Cited by 67 publications

(59 citation statements)

References 21 publications

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“…In addition, the results of live and dead staining showed that only a few of cells were dead. Kikuchi et al [29] found that there were thickness limitations in maintaining tissues in a static culture. Three-layer human skeletal muscle myoblast (HSMM) sheets maintained their live cell number.…”

Section: Discussionmentioning

confidence: 99%

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang

Lai

et al. 2017

BioMed Research International

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Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

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Section: Discussionmentioning

confidence: 99%

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang

Lai

et al. 2017

BioMed Research International

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“…Taking inkjet printing as an example, although droplet ejection frequencies of around 20 kHz have been achieved [40], ejection of cells can induce transient nanopores in printed cells [41], which may explain some of the cell damage observed in this process. Just-in-time cell harvesting, microfluidic culture devices, or automated cell sheet manipulation [42] may improve the scalability and complexity of construct fabrication.…”

Section: D Printing: Engineering Layer-by-layermentioning

confidence: 99%

The Billion Cell Construct: Will Three-Dimensional Printing Get Us There?

Miller

2014

PLoS Biol

168

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“…Generally, there are two methods for constructing engineered cardiac tissue. 8 Cell sheet technology has been well-studied, and clinical trials in the myocardium are underway. 3,4 The core principle is to culture cells on a thermoresponsive polymer (i.e.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of optogenetic, multi-strip cardiac muscles

et al. 2015

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Cardiac tissue engineering aims to recreate functional tissue constructs similar to the structure and function of the native myocardium. To date, in vitro tissue constructs lack the architectural complexity of a vascular network and the precise motor unit control of muscle fibers. Here, we present a method to construct engineered multi-strip cardiac muscle that simulates the bundle-like architecture of the native myocardium. Densely packed primary myocytes and cardiac fibroblasts were co-cultured with optogenetic, non-excitable cells. The resulting 3D syncytium triggered contraction upon localized blue light illumination to selectively activate and pace the multi-strip cardiac muscles, similar to the activity of pacemaker cells. Acting on a single load, we demonstrated graded force production through light-modulated multi-strip recruitment. These results demonstrate an in vitro platform of optogenetic, multi-strip cardiac muscles that can be used in a wide variety of applications, such as drug discovery, tissue engineering, and bio-hybrid robotic systems.

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Automatic fabrication of 3-dimensional tissues using cell sheet manipulator technique

Cited by 67 publications

References 21 publications

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

The Billion Cell Construct: Will Three-Dimensional Printing Get Us There?

Fabrication and characterization of optogenetic, multi-strip cardiac muscles

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