Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang, Zhiwei; Xi, Yue; Lai, Kaichen; Wang, Ying; Wang, Huiming; Yang, Guoli

doi:10.1155/2017/9474573

Cited by 17 publications

(14 citation statements)

References 36 publications

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“…Natural organ ECM represents an important substrate formed from a complex of highly ordered fibrous proteins which provides many biological properties known to support cell viability and growth. Whole organ decellularized pancreatic ECM has been derived from mice, rats, pigs and humans 39 – 45 as a potential alternative to traditional organ transplantation where relevant cell types are reseeded into the decellularized matrix with the hope of reconstituting a neo-organ with functional properties. However, recellularizing and rebuilding the vasculature of intact decellularized organ scaffolds can be challenging 39 , 41 , 43 , 45 – 47 .…”

Section: Discussionmentioning

confidence: 99%

Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas

Sackett

Tremmel

et al. 2018

Sci Rep

224

164

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Extracellular matrix (ECM) plays an important developmental role by regulating cell behaviour through structural and biochemical stimulation. Tissue-specific ECM, attained through decellularization, has been proposed in several strategies for tissue and organ replacement. Decellularization of animal pancreata has been reported, but the same methods applied to human pancreas are less effective due to higher lipid content. Moreover, ECM-derived hydrogels can be obtained from many decellularized tissues, but methods have not been reported to obtain human pancreas-derived hydrogel. Using novel decellularization methods with human pancreas we produced an acellular, 3D biological scaffold (hP-ECM) and hydrogel (hP-HG) amenable to tissue culture, transplantation and proteomic applications. The inclusion of a homogenization step in the decellularization protocol significantly improved lipid removal and gelation capability of the resulting ECM, which was capable of gelation at 37 °C in vitro and in vivo, and is cytocompatible with a variety of cell types and islet-like tissues in vitro. Overall, this study demonstrates the characterisation of a novel protocol for the decellularization and delipidization of human pancreatic tissue for the production of acellular ECM and ECM hydrogel suitable for cell culture and transplantation applications. We also report a list of 120 proteins present within the human pancreatic matrisome.

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Section: Discussionmentioning

confidence: 99%

Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas

Sackett

Tremmel

et al. 2018

Sci Rep

224

164

View full text Add to dashboard Cite

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“…Isolation and Culture of BMSCs : BMSCs were isolated in accordance with the previously described method with minor amendment . The rats (3 weeks old) were killed through cervical dislocation and soaked in 75% alcohol for 2 min.…”

Section: Methodsmentioning

confidence: 99%

PGS Scaffolds Promote the In Vivo Survival and Directional Differentiation of Bone Marrow Mesenchymal Stem Cells Restoring the Morphology and Function of Wounded Rat Uterus

Xiao

Yang

Lei

et al. 2019

Adv Healthcare Materials

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Intrauterine adhesion (IUA) causing infertility and recurrent miscarriage of reproductive female mammals usually results from endometrium injury. Nevertheless, there is no efficient therapeutic method to avoid IUA. Bone marrow derived mesenchymal stem cells (BMSCs) are an important cell source for tissue regeneration. This study designs and explores the ability of BMSC‐loaded elastic poly(glycerol sebacate) (PGS) scaffold to prevent IUA and compares the effect of PGS with poly(lactic‐co‐glycolic acid) (PLGA) and collagen scaffolds in resumption of damaged rat uteruses. The 3D architecture provided by PGS scaffolds favors the attachment and growth of rat BMSCs. In vivo bioluminescence imaging shows that compared with direct BMSC intrauterine injection, PLGA, and collagen scaffolds, the PGS scaffold significantly prolongs the retention time of BMSCs in a wounded rat uterus model. More importantly, BMSCs can directly differentiate into endometrial stromal cells after transplantation of PGS/BMSCs constructs, but not PLGA/BMSCs and collagen/BMSCs. It is found that the level of transforming growth factor β1 (TGF‐β1), basic fibroblast growth factor (bFGF), vascular endothelial growth factor, and insulin‐like growth factors in the injured endometrium adjacent to PGS/BMSCs constructs is higher than those of rats receiving PLGA/BMSCs, collagen/BMSCs, or BMSCs intrauterine transplantation. Besides, transplantation of PGS/BMSCs leads to better morphology recovery of the damaged uterus than PLGA/BMSCs and collagen/BMSCs. The receptive fertility of PGS/BMSCs is 72.2 ± 6.4%, similar to the one of collagen/BMSCs, but significantly higher than 42.3 ± 3.9% in PLGA/BMSCs. Taken together, PGS/BMSCs may be a promising candidate for preventing IUA.

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“…Wei et al reported that Vitamin C treatment promotes in vitro mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity [ 33 ]. Jiang et al reported that methods for cell sheet harvesting include temperature-, electricity-, magnetism-, and pH change-induced methods and suggested a new strategy to obtain MSC sheets using light-induced cell sheet technology [ 34 ]. Using this technology, intact MSC sheets were detached from TiO 2 nanodot-coated quartz substrate after UV365 illumination.…”

Section: Mesenchymal Stem Cells and Their Use In Cell Sheet-based mentioning

confidence: 99%

A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

Yörükoğlu

Kiter

Akkaya

et al. 2017

Stem Cells International

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The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.

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Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Cited by 17 publications

References 36 publications

Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas

Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas

PGS Scaffolds Promote the In Vivo Survival and Directional Differentiation of Bone Marrow Mesenchymal Stem Cells Restoring the Morphology and Function of Wounded Rat Uterus

A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

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