Automatic Identification and Separation of Somatic Embryos in Vitro

Harrell, R. C.; Hood, C.F.; Moltó, Eduardo; Munilla, R.; Bieniek, Marianne E.; Cantliffe, Daniel J.

doi:10.17660/actahortic.1992.319.95

Cited by 7 publications

(2 citation statements)

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“…The advantages of liquid media in enhancing the shoot propagation (Harris and Manson, 1983;Alvard et al, 1993;Levin et al, 1999), growth (Snir and Erez, 1980) and somatic embryogenesis (Jones and Petolino, 1988;Harrel et al, 1994;Liu et al, 1998;Hosokawa et al, 1998) have been reported for several plant species. However, the use of liquid media can lead to the problem of asphyxia in explants as a result of immersion.…”

Section: Introductionmentioning

confidence: 99%

Comparison of liquid culture methods and effect of temporary immersion bioreactor on growth and multiplication of banana (Musa, cv. Dwarf Cavendish)

Farahani¹,

Majd²

2012

Afr. J. Biotechnol.

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Four different liquids, as well as solid culture methods used in shoot propagation of banana were compared. Treatments studied were solid medium (A), liquid medium with immersion of plants (B), liquid medium with cotton culture support (C), liquid medium aerated by bubbling (D), and liquid medium with a temporary immersion bioreactor system (TIB) for 20 min every 1 h (E). After 4 weeks of culture, shoots in liquid medium with immersion and liquid medium aerated by bubbling showed none too little proliferation. Shoots in the solid medium and those cultured in liquid medium containing cotton culture supported played multiplication rates of 2.7 to 3.5 with the highest multiplication rate (> 7.00) observed in the explants that were subjected to the TIB in the medium. Three treated groups differed in the accumulation of dry matter; the lowest weight (around 0.6 g) was observed in treatments B and D, while 2 to 4 times greater accumulation was observed in the explants in the solid medium and those cultured in the liquid medium with a cotton culture support. The highest multiplication rates and weight gains were observed in the liquid medium with a TIB (E). Shoots in liquid medium continuously aerated by bubbling, displayed hyperhydricity of the outer leaf sheaths. However, this was not observed with temporary immersion of explants.

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Section: Introductionmentioning

confidence: 99%

Comparison of liquid culture methods and effect of temporary immersion bioreactor on growth and multiplication of banana (Musa, cv. Dwarf Cavendish)

Farahani¹,

Majd²

2012

Afr. J. Biotechnol.

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“…It gave a classification accuracy of about 80 to 90% for different normal embryo classes. A machine vision based neural network classifier has also been developed to identify mature embryos for harvesting (12). It demonstrates the possibility of using such a system in artificial seed productions.…”

Section: Introductionmentioning

confidence: 99%

Classification of Plant Somatic Embryos by Using Neural Network Classifiers

Ruan

Zhang

et al. 1997

Biotechnol. Prog.

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The development of somatic embryos is characterized by a series of morphological changes. Quantitative kinetic studies have been hampered by the difficulties in enumerating and characterizing embryo populations. By employing neural networks, we have developed a pattern‐recognition system for characterizing the morphological features of carrot somatic embryos. This pattern‐recognition system employs a hierarchical decision tree to achieve optimal classification. It successfully classified carrot somatic embryos into normal and abnormal embryo classes. For normal embryo classes (globular, oblong, heart, and torpedo embryos), an accuracy of 90% or higher was achieved. The features identified by the neural network classifiers as most important for embryo classification are almost identical to those obtained by the branch‐and‐bound searching algorithm used previously. However, employing the neural networks shortens the system developing time greatly. Coupled with an image analysis system, this neural‐network‐based pattern‐recognition system shows great potential in embryo sorting and automation of synthetic seed production.

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