Automatic process control for stable bio-hythane production in two-phase thermophilic anaerobic digestion of food waste

Mıcoluccı, Federico; Gottardo, Marco; Bolzonella, David; Pavan, Paolo

doi:10.1016/j.ijhydene.2014.08.136

Cited by 94 publications

(42 citation statements)

References 14 publications

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“…FW was used as the substrate as it has been proven to be a highly desirable feedstock for anaerobic fermentation, due to its high biodegradability, availability, and well balanced carbon and nutrient contents [11,18,34]. FW was manually sorted from OFMSW collected in a Tuscan municipality (Italy) by means of a kerbside collection system.…”

Section: Substratesmentioning

confidence: 99%

Biochemical Hydrogen Potential Tests Using Different Inocula

2019

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Four inocula collected from different operating facilities were tested in their hydrogenic performances by means of two biochemical hydrogen potential test set-ups using sucrose and food waste as substrates, with the aim of evaluating the influence of inoculum media in batch fermentative assays. The selected inocula were: activated sludge collected from the aerobic unit of a municipal wastewater treatment plant, digested sludge from an anaerobic reactor treating organic waste and cattle manure, digested sludge from an anaerobic reactor treating agroindustrial residues, and digested sludge from an anaerobic reactor of a municipal wastewater treatment plant. Test results, in terms of specific hydrogen production, hydrogen conversion efficiency, and volatile solids removal efficiency, were significantly dependent on the type of inoculum. Statistical analysis showed different results, indicating that findings were due to the different inocula used in the tests. In particular, assays performed with activated sludge showed the highest performances for both substrates and both experimental set-ups.

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Section: Substratesmentioning

confidence: 99%

Biochemical Hydrogen Potential Tests Using Different Inocula

2019

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“…The 2 nd phase of anaerobic decomposition is dominated by a group of methanogenic bacteria, which grow in neutral condition (pH 6.7-7.5) [7]. Thus, the pH of the 2 nd stage medium was adjusted to 7 as the substrate (the liquid part) from the 1st stage had a low pH.…”

Section: Production Of Methane In the 2 Nd Stage Of Anaerobic Digestimentioning

confidence: 99%

Anaerobic digestion of citrus waste using two-stage membrane bioreactor

Millati

Lukitawesa

Permanasari

et al. 2018

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. Anaerobic digestion is a promising method to treat citrus waste. However, the presence of limonene in citrus waste inhibits anaerobic digestion process. Limonene is an antimicrobial compound and could inhibit methane forming bacteria that takes a longer time to recover than the injured acid forming bacteria. Hence, volatile fatty acids will be accumulated and methane production will be decreased. One way to solve this problem is by conducting anaerobic digestion process into two stages. The first step is aimed for hydrolysis, acidogenesis, and acetogenesis reactions and the second stage is aimed for methanogenesis reaction. The separation of the system would further allow each stage in their optimum conditions making the process more stable. In this research, anaerobic digestion was carried out in batch operations using 120 ml-glass bottle bioreactors in 2 stages. The first stage was performed in free-cells bioreactor, whereas the second stage was performed in both bioreactor of free cells and membrane bioreactor. In the first stage, the reactor was set into 'anaerobic' and 'semi-aerobic' conditions to examine the effect of oxygen on facultative anaerobic bacteria in acid production. In the second stage, the protection of membrane towards the cells against limonene was tested. For the first stage, the basal medium was prepared with 1.5 g VS of inoculum and 4.5 g VS of citrus waste. The digestion process was carried out at 55°C for four days. For the second stage, the membrane bioreactor was prepared with 3 g of cells that were encased and sealed in a 3×6 cm 2 polyvinylidene fluoride membrane. The medium contained 40 ml basal medium and 10 ml liquid from the first stage. The bioreactors were incubated at 55°C for 2 days under anaerobic condition. The results from the first stage showed that the maximum total sugar under 'anaerobic' and 'semi-aerobic' conditions was 294.3 g/l and 244.7 g/l, respectively. The corresponding values for total volatile fatty acids were 3.8 g/l and 2.9 g/l, respectively. Methane production of citrus waste taken from the first stage under 'anaerobic' condition in membrane and free-cells bioreactors was 11.2 Nml and 7.2 Nml, respectively. Whereas, methane production of citrus waste taken from the first stage under 'semi-aerobic' condition in membrane and free-cells bioreactors was 8.8 Nml and 5.7 Nml, respectively. It can be seen from the results of the first stage that volatile fatty acids from 'anaerobic' condition was higher than that of 'semi-aerobic' condition. The absence of oxygen provides the optimal condition for growth and metabolism of facultative and obligatorily anaerobic bacteria in the first stage. Furthermore, polyvinylidene fluoride membrane was able to protect the cells from antimicrobial compounds.

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“…In another pilot scale trial study [84], simultaneous production of hydrogen and methane for a trial period of 310 d in a two-phase thermophilic anaerobic digestion process utilizing FW as sole substrate was demonstrated. The biohythane had a mix of 7% H 2 , 58% CH 4 and 35% CO 2 while the average specific gas production was 0.69 m 3 biogas/kgTVS and gas production rate of 2.78 m 3 m À 3 d. A prototype of on-site biogas production from high-rise building as a single-stage anaerobic digester for the codigestion of FW and sewage sludge was proposed [85].…”

Section: Cases Of Pilot-scale Operationsmentioning

confidence: 99%