Abstract-Electromagnetic shielding (EMS) fabrics often need to design rectangular holes for application. However, there is not a mature approach to predict the shielding effectiveness (SE) of the EMS fabric with rectangular hole. This paper proposes that there are a number of loose regions of conductive fibers on the hole edge of the EMS fabric, and establishes a SE prediction model of the EMS fabric with rectangular hole. Firstly, the loose region of conductive fiber is analyzed to build a model of the rectangular hole. Secondly, the SE prediction model of the EMS fabric with rectangular hole is deduced according to the transmission coefficient of the normal region, hole region and loose region, and the determining method of the loose region is given. Finally, the prediction model is verified by experiments. The results show that the model can successfully predict the SE of the EMS fabric with the plain, twill and satin weaves, and the factors such as frequency, fabric density and metal fiber content have little influence on the model. The proposed model can provide a valuable reference for the rational design of the rectangular hole of the EMS fabric.