Autoradiographic analyses of the effects of estradiol benzoate on [3H]paroxetine binding in the cerebral cortex and dorsal hippocampus of gonadectomized male and female rats

Mendelson, Scott D.; McKittrick, Christina R.; McEwen, Bruce S.

doi:10.1016/0006-8993(93)91724-7

Cited by 45 publications

(22 citation statements)

References 17 publications

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“…In male rats, castration decreased the density o f 5-H Tia receptors in the M P O A and this is in complete agreement with a pre vious study in which testosterone administration in creased 5-HTia binding in the PO A o f castrated rats [28]. Paroxetine binding was not measured in this study be cause it had been determined previously by this lab that E increases [3H]paroxetine binding in the hippocampus but does not alter [3H]paroxetine in the hypothalamus [13;Mendelson,unpubl. observations].…”

Section: Discussionsupporting

confidence: 78%

Effect of 5,7-Dihydroxytryptamine, Ovariectomy and Gonadal Steroids on Serotonin Receptor Binding in Rat Brain

et al. 1994

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Quantitative autoradiography was used to assess alterations in serotonin (5-HT) receptor binding in the hypothalamus and hippocampus following denervation with 5, 7-dihydroxytryptamine (5, 7-DHT), ovariectomy (OVX) and gonadal steroid manipulation. Seven days after 5, 7-DHT injection, 5-HT_1a receptor density was significantly increased in the ventromedial hypothalamic nucleus (VMN) of intact but not OVX femaled rats. Under these conditions 5-HT_1b receptor density was unchanged in any brain region examined and 5-HT transporter binding was decreased in all 5, 7-DHT injected animals. In addition, there was a significant interaction between OVX and 5, 7-DHT for both the 5-HT_1a receptor and the 5-HT transporter in the VMN, such that OVX inhibited the 5, 7-DHT-induced increase in 5-HT_1a receptors and attenuated the 5, 7-DHT-induced decrease in 5-HT transporter binding. In a separate experiment the effect of gonadal steroid manipulation on 5-HT receptor binding was assessed. In female OVX rats, 5-HT_1a receptor density was unchanged by estrogen or estrogen and progesterone administration. In male rats, castration significantly decreased 5-HT_1a receptor density in the medial preoptic area. Estrogen and progesterone administration to female OVX rats increased the density of 5-HT_1b receptors in the VMN, as compared to estrogen alone. The relationship of these results to the role of 5-HT in mediating lordosis behavior is discussed.

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Section: Discussionsupporting

confidence: 78%

Effect of 5,7-Dihydroxytryptamine, Ovariectomy and Gonadal Steroids on Serotonin Receptor Binding in Rat Brain

et al. 1994

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“…Interestingly, in the FST, raloxifene induced antidepressant-like effects similar to SSRIs such as fluoxetine (Estrada-Camarena et al, 2003;Estrada-Camarena et al, 2010). Studies in rats, analyzing different brain areas, indicate that acute or chronic treatment with estradiol benzoate decreased the number of 3 [H] paroxetine binding sites (Mendelson et al, 1993). These results are in agreement with in vitro studies that reported that some estrogenic compounds interact with the serotonin transporter in membranes obtained from cerebral cortex, hippocampus, hypothalamus and striatum (Chang & Chang, 1999).…”

Section: Evidence Of Estrogens Interactions With the Serotonegic Systemsupporting

confidence: 83%

Participation of the Monoaminergic System in the Antidepressant-Like Actions of Estrogens: A Review in Preclinical Studies

López‐Rubalcava¹,

Vega‐Rivera²,

Páez-Martínez³

et al. 2012

Effects of Antidepressants

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“…Reports analyzing the action of estrogen in vivo on SERT uptake sites and mRNA levels are inconclusive. Increases, decreases, and no change have been reported (Attali et al, 1997;Bethea et al, 1998;Krajnak et al, 2003;McQueen et al, 1997;Mendelson et al, 1993, Zhou et al, 2002. Discrepancies in these studies may be explained by variability in the treatment paradigm used, such as the time elapsed between ovariectomy and estrogen treatment, the duration of estrogen treatment, and the brain region evaluated.…”

Section: Introductionmentioning

confidence: 79%

Impact of Ovarian Hormones on the Modulation of the Serotonin Transporter by Fluvoxamine

Benmansour

Piotrowski

Altamirano

et al. 2008

Neuropsychopharmacol

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Most preclinical studies examining the mechanism(s) of action of antidepressants are carried out using male animals. Blockade of serotonin transporter (SERT) function by selective serotonin reuptake inhibitors (SSRIs) is the initial event that triggers a not completely understood process that results in clinical improvement in depression. To investigate whether there are differences in the ability of SSRIs to inhibit the SERT between male and female rats at different phases of the estrous cycle, clearance of locally applied serotonin (5-HT) was measured by in vivo chronoamperometry. Local application of the SSRI, fluvoxamine, directly into the CA3 area of hippocampus increased significantly 5-HT clearance time parameters in male rats and female rats in estrus or diestrus, but not in proestrus. The contribution of ovarian steroids to this result was investigated in ovariectomized (OVX) rats treated with estradiol benzoate (EB) and/or progesterone (P). In OVX-control rats, fluvoxamine increased clearance time parameters, whereas EB and/or P treatment blocked this effect, consistent with what was seen in female rats in proestrus. This effect was gender-specific, since treatment of castrated rats with EB/ P had no effect on the ability of fluvoxamine to slow 5-HT clearance. The time course of hormonal effects showed that 1-60 min after local application of 17-b-estradiol (E 2 ) into the CA3 region of OVX rats, fluvoxamine had no effect on clearance time of 5-HT. E 2 -BSA mimicked E 2 's effects at 10 min but not at 60 min. Pretreatment with estrogen receptor antagonists blocked the effects of E 2 . The finding that acutely both estradiol and progesterone can inhibit the ability of an SSRI to slow the clearance of 5-HT, may have important implications for the use of SSRIs in women.

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Autoradiographic analyses of the effects of estradiol benzoate on [3H]paroxetine binding in the cerebral cortex and dorsal hippocampus of gonadectomized male and female rats

Cited by 45 publications

References 17 publications

Effect of 5,7-Dihydroxytryptamine, Ovariectomy and Gonadal Steroids on Serotonin Receptor Binding in Rat Brain

Effect of 5,7-Dihydroxytryptamine, Ovariectomy and Gonadal Steroids on Serotonin Receptor Binding in Rat Brain

Participation of the Monoaminergic System in the Antidepressant-Like Actions of Estrogens: A Review in Preclinical Studies

Impact of Ovarian Hormones on the Modulation of the Serotonin Transporter by Fluvoxamine

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