Autoradiographic Investigation of Incorporation of H
            <sup>3</sup>
            -Thymidine into Cells of the Rat and Mouse

Schultze, B.; Oehlert, W.

doi:10.1126/science.131.3402.737

Cited by 138 publications

(15 citation statements)

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“…1). This protocol can be used as an efficient screening procedure for a large number of bacterial clones pre- 1 To whom correspondence should be addressed. Fax: 55 41 266 2042.…”

Section: Resultsmentioning

confidence: 99%

“…Conventional proliferation assays are based on the measurement of DNA synthesis by the incorporation of labeled nucleosides. The incorporation of radioactively labeled [ 3 H]thymidine into DNA has been the most common and earliest developed measure of mitosis and cell proliferation (1). A nonradioactive proliferation assay based on the incorporation of the pyrimidine nucleoside analogue BrdU 2 instead of thymidine during DNA synthesis and its subsequent immunohistochemical detection was later developed (2,3).…”

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confidence: 99%

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Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Wittstock

Rehfeldt

Mix

et al. 2001

Analytical Biochemistry

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“…1). This protocol can be used as an efficient screening procedure for a large number of bacterial clones pre- 1 To whom correspondence should be addressed. Fax: 55 41 266 2042.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Wittstock

Rehfeldt

Mix

et al. 2001

Analytical Biochemistry

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“…By the use of the mitotic indices obtained at the various intervals of time, the cycle of the islet cells can be estimated roughly, by the methods of Schultze and Oehlert 36 and Wimber. 37 The mean nuclear cycle of the islet in the normal, untreated mice is about 10.6 hrs., the time for DNA synthesis about 5.8 hrs., and the time for mitosis 0.4 hrs., or 24 min.…”

Section: Discussionmentioning

confidence: 99%

“…The nuclear cycle of the islet cells may be calculated from table 3 by using the methods of Schultze and Oehlert 36 and Wimber 37 as follows:…”

Section: The Interrelationships Of the Pancreatic Ducts Blood Vesselmentioning

confidence: 99%

Postnatal Neogenesis of Islets of Langerhans in the Mouse

Bunnag

1966

Diabetes

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The source of the new islets during growth and the effect of aging on the number of islets have been studied in Webster Swiss mice. A combination of methods was employed, including microscopy in vivo, enumeration of the total number of islets, radioautography with H-3-thymidine, and conventional histological technics. The total number of islets in normal mice increases with age until about six months, and then becomes stable or decreases slightly. Radioautographs showed that the cells of the islets are in all likelihood derived from migrating ductal or periductal cells residing at the junction between the islet and pancreatic duct and at the periphery of the islet, and that these cells doubtless serve as the progenitor pool for the growth of the islet. The nuclear cycle of the insular cells was also determined by this method. In vivo study revealed that the structural integrity of ducts and of their vascular supply is important for the maintenance of the population of ductal and periductal islets. DIABETES 75:480-91, July, 1966.Regeneration of pancreatic tissue and formation of new islets of Langerhans in adult mammals of various species have been described in a variety of experimental conditions, including partial or complete pancreatectomy, 1 " 7 ligation of the pancreatic duct, 8 ' 9 ligation of the pancreatic arteries, 10 prolonged hyperglycemia produced by intravenous infusion of glucose, 11 protracted glucagon administration, 12 force-feeding of a high carbohydrate diet 13 and the administration of alloxan. 14 " 16 Whether new islets of Langerhans are formed in the resting pancreas of adult mammals under normal conditions is disputed, 7 " 22 and information concerning the formation of islets in aging is scanty.In both the experimental and the normal state, the

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“…In autoradiographic studies of the gas trointestinal tract, smooth muscle is usually ignored or is the subject of only few com ments [9,23,[25][26][27], The muscularis ex terna can be classified as a slowly renewing population. In adult rabbit more than 0.3% of the cells of the colonic muscularis externa were heavily labeled 2 h after a multipledose pulse [10].…”

Section: Discussionmentioning

confidence: 99%

Cell Proliferation in Developing Human Jejunum

Arsenault¹,

Ménard²

1987

Neonatology

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Cell proliferation of the developing human jejunum was investigated by radioautography using organ culture. The sites of [³H]-thymidine uptake were localized in the epithelium, the mesenchyme and the muscularis externa of fetal human small intestinal explants from 8 to 18 weeks of gestation. Proliferating cells were abundant and scattered throughout the stratified epithelium before the appearance of villi. Many nuclei in the mesenchyme and the muscular layer were labeled. With the villi formation and the simplification of the epithelium, there was a confinement of the proliferating zone in the intervillus areas and developing crypts. The quantitation of proliferating cells showed a labeling index at its highest value between 8 and 10 weeks of gestation decreasing gradually up to 18 weeks of gestation at the epithelial and mesenchymal level. In the muscularis externa, the labeling remained more or less constant between 11 and 18 weeks of gestation. The organ culture of intestinal explants for 5 days did not modify epithelial cell proliferation. The present investigation provides for the first time basic quantitative data regarding cell proliferation in the developing human jejunum.

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Autoradiographic Investigation of Incorporation of H ³ -Thymidine into Cells of the Rat and Mouse

Cited by 138 publications

References 0 publications

Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Postnatal Neogenesis of Islets of Langerhans in the Mouse

Cell Proliferation in Developing Human Jejunum

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Autoradiographic Investigation of Incorporation of H 3 -Thymidine into Cells of the Rat and Mouse

Cited by 138 publications

References 0 publications

Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Comparison of Three Different Proliferation Assays for Mouse Myoblast Cultures

Postnatal Neogenesis of Islets of Langerhans in the Mouse

Cell Proliferation in Developing Human Jejunum

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Product

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Autoradiographic Investigation of Incorporation of H ³ -Thymidine into Cells of the Rat and Mouse