Auxin production by the Klebsiella planticola strain TSKhA-91 and its effect on development of cucumber (Cucumis sativus L.) seeds

Blinkov, E. A.; Цавкелова, Е. А.; Selitskaya, O. V.

doi:10.1134/s0026261714050063

Cited by 18 publications

(16 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was also observed that there was a relationship between IAA production and the growth of the B. cereus strains. Results obtained in the present study were also confirmed by Prashanth and Mathivanan [20] and Blinkov et al [21]. …”

Section: Screening Of B Cereus Isolates For Iaa Productionsupporting

confidence: 91%

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Özdal¹,

Ozdal²,

Sezen³

et al. 2016

CSJ

View full text Add to dashboard Cite

Abstract. Four different strains of Bacillus cereus producing indole-3-acetic acid (IAA) were isolated from various rhizospheric soils and characterized biochemically. The isolates were screened for (IAA) production and BC-On strain was found to be the best IAA producer with 46.25 mg/l. Maximum IAA production was obtained in the stationary growth phase at 72h. Significant correlation was also observed between IAA production and growth of the B. cereus strains. Among the isolates, BC-On strain was further used for immobilization studies. Maximum IAA production was obtained at initial pH of 8.0 and temperature of 35 °C after 18 h of fermentation. The immobilized cells could be effectively reused thirteen times and the IAA concentration of 300 mg/l was determined during this period. Results showed that immobilized cells can be used in the continuous process for the production of IAA. The productivity obtained by immobilization was higher than the one obtained by submerged cultivation and immobilization reduced the fermentation time.Keywords: Indole-3-acetic acid, Rhizobacteria, Bacillus cereus, Immobilization Immobilize Bacillus cereus Hücreleri Tarafından İndol-3-Asetik Asitin BiyosenteziÖzet. İndol-3-asetik asit (İAA) üreten dört farklı Bacillus cereus straini çeşitli rizosfer topraklarından izole edildi ve biyokimyasal olarak karakterize edilmiştir. İzolatlar İAA üretimi için taranmış ve BC-On straini 46.25 mg/l ile en iyi İAA üreticisi olarak bulunmuştur. En fazla IAA üretimi 72. saatte durağan büyüme fazında elde edilmiştir. B. cereus strainlerinin IAA üretimi ve büyüme arasında önemli bir ilişki gözlenmiştir. İzolatlar arasında, BC-On straini immobilizasyon çalışmalarında kullanılmıştır. En fazla IAA üretimi 18 saatlik fermentasyon sonrasında başlangıç pH'sı 8.0 ve 35 °C elde edilmiştir. İmmobilize edilmiş hücreler etkili olarak on üç kez kullanılabilmiş ve bu süre boyunca 300 mg/l İAA konsantrasyonu tespit edilmiştir. Sonuçlar immobilize hücrelerin IAA üretimi için sürekli bir işlemde kullanılabilir olduğunu göstermiştir. İmmobilizasyon ile elde edilen verimlilik, derin fermentasyon ile elde edilenden daha yüksek olduğu bulunmuş ve immobilizasyon fermentasyon süresini kısaltmıştır.

show abstract

Section: Screening Of B Cereus Isolates For Iaa Productionsupporting

confidence: 91%

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Özdal¹,

Ozdal²,

Sezen³

et al. 2016

CSJ

View full text Add to dashboard Cite

show abstract

“…The maximal IAA production for diazotrophic K. oxytoca TSKhA-gfp was observed in nitrogen free medium (120 lg ml À1 ); rhizobacteria are known to produce high amounts of auxin in unfavourable conditions that might induce the formation of plant-microbial associations (Oljunina and Shabaev 1996). The wild strain of the tested Klebsiella also demonstrated the highest production in the same nitrogen-free conditions (Blinkov et al 2014) in amounts of around 80 lg ml À1 . In this previous study, the feeding experiments with exogenous IAM supplementation did not show any consumption or its transformation into IAA by the wild Klebsiella strain.…”

Section: Discussionmentioning

confidence: 80%

“…The wild strain of the tested Klebsiella also demonstrated the highest production in the same nitrogen‐free conditions (Blinkov et al . ) in amounts of around 80 μ g ml −1 . In this previous study, the feeding experiments with exogenous IAM supplementation did not show any consumption or its transformation into IAA by the wild Klebsiella strain.…”

Section: Discussionmentioning

confidence: 99%

“…no AP014951.1). Previously (Blinkov et al 2014), we showed that the wild type of K. oxytoca (former K. planticola TSKhA-91) produced the highest IAA yield at the stationary phase of growth under the tryptophan supplementation, producing up to 85Á5 lg ml À1 of auxins in the nitrogen-free medium. In order to estimate the IAA production by its gfp-tagged transformant, K. oxytoca TSKhA-gfp as well as P. fluorescens 32-gfp, were also tested for their capacity of auxin production with various nitrogen sources.…”

Section: Production Of the Plant Growth Stimulator Iaamentioning

confidence: 99%

See 1 more Smart Citation

Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

et al. 2017

View full text Add to dashboard Cite

show abstract

“…It has been shown that rhizobacteria support plant growth under stressful conditions by improving their mineral nutrition, water relations, resistance to pathogens and antioxidant functions [ 5 , 6 , 7 , 8 , 9 ]. The capacity of rhizoshere bacteria to produce hormones is considered as an important mechanism promoting plant growth and productivity under both favorable and stress conditions [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. Plant hormone signaling plays an important role in many physiological and developmental processes, including stress response.…”

Section: Introductionmentioning

confidence: 99%

Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

et al. 2020

View full text Add to dashboard Cite

Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoculation with either bacterium promoted the growth in terms of leaf mass accumulation. The effects were associated with increased concentrations of auxin and cytokinin hormones in the leaves and stems and with suppression of an increase in the leaf abscisic acid that PEG treatment otherwise promoted in the potato microplants. O. cytisi IPA7.2 had a greater growth-stimulating effect than A. brasilense Sp245 on stressed plants, while A. brasilense Sp245 was more effective in unstressed plants. The effects were likely to be the result of changes to the plant’s hormonal balance brought about by the bacteria.

show abstract

Auxin production by the Klebsiella planticola strain TSKhA-91 and its effect on development of cucumber (Cucumis sativus L.) seeds

Cited by 18 publications

References 15 publications

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

Contact Info

Product

Resources

About