Avaliação da eficiência do Somaticell® para o diagnóstico da contagem indireta de células somáticas no leite de búfalas

Abstract

Abstract:Two experiments were conducted with a view to evaluating the Somaticell® diagnosis involving an indirect somatic cell-count in buffalo milk. In experiment A, six Murrah buffaloes in different lactation phases were subjected to Somaticell®, CMT and Alizarol tests. In experiment B, 49 Murrah buffaloes in different lactation phases were used, giving a total of 196 samples, on which Somaticell® and CCS tests were carried out using an electronic counter. It was observed that the counting of somatic cells performed using Somaticell® provided values significantly higher than those obtained when using an electronic counter. The values obtained using the Somaticell® test were 4.7 times higher than those obtained for the same samples by way of electronic counting of somatic cells. Comparison of Somaticell® and CCS electronic counting for each mammary gland separately revealed a disparity between the mean values obtained using both techniques, with the lowest values obtained using Somaticell® 69 times higher than those obtained using CCS. The correlations between both tests were low and insignificant, demonstrating that the techniques behave differently when counting the somatic cells in buffalo milk.Viana et al. / Rev. Ci. Agra., v.53, n.

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

“…Mastitis is an inflammation of the mammary gland parenchyma due to an infectious process predominantly caused by many microorganisms, particularly bacteria, and may also be involved fungi and yeast (Baloch et al 2011). The effective and accurate diagnosis is extremely important to control this severe disease in buffalo (Viana et al 2010). However, the absence of macroscopic changes in the tissues or secretions in cases of subclinical mastitis, does not allow the identification of infected mammary quarters before milking, once routine diagnostic methods include only physical examination, and fluid secretion (Bonini Pardo et al 2007).…”

Quimiometria na classificação de leite cru refrigerado

“…Para avaliar a qualidade microbiológica, as amostras foram refrigeradas a temperatura de ± 3 ºC e acondicionadas em frascos estéreis de polietileno. Em seguida, foram transportadas e analisadas seguindo os métodos oficiais estabelecidos pela Instrução Normativa nº 62/2003 do Ministério da Agricultura, Pecuária e Abastecimento -MAPA [16] para determinar a contagem de bactérias totais (CBT) e para a contagem de células somáticas (CCS) foi utilizada a metodologia proposta por Viana et al [17] .…”