Avaliação da fase inicial do reparo ósseo após implantação de biomateriais

Almeida, Renata dos Santos; Ribeiro, Iorrana Índira dos Anjos; Silva, Marcelo Henrique Prado da; Rocha, Daniel Navarro da; Miguel, Fúlvio Borges; Rosa, Fabiana Paim

doi:10.9771/cmbio.v13i3.12940

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…This demonstrates that the scaffolds have physical-chemical characteristics, topography, 3D architecture and porosity favorable to tissue neoformation, as proposed by Karageorgiou and Kaplan [7] and Tumedei, Savadori and Del Fabbro [1] and demonstrated in previous studies [23,32,33]. Furthermore, throughout the experiment, these scaffolds were not encapsulated, unlike the studies that evaluated particulate or microsphere biomaterials [29,[38][39][40][41]. Encapsulation would cause the scaffolds to have limited regenerative potential, with the formation of fibrosis surrounding biomaterials.…”

Section: Discussionmentioning

confidence: 55%

“…In this condition, tissue repair occurs through fibrosis with reduced thickness due to the absence of 3D scaffold, essential to regeneration [2]. In this study, no control group with bone defect was used without implantation of the scaffold, in consonance with the principles of the 3R's (Replacement, Reduction and Refinement) and with results established in the literature [35][36][37] and found by our group [2,29,38,39].…”

Section: Discussionmentioning

confidence: 58%

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Bone regeneration using Wollastonite/β-TCP scaffolds implants in critical bone defect in rat calvaria

Santos¹,

Miguel²,

Barbosa³

et al. 2021

Biomed. Phys. Eng. Express

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In order to provide favorable conditions for bone regeneration, a lot of biomaterials have been developed and evaluated, worldwide. Composite biomaterials have gained notoriety, as they combine desirable properties of each isolated material. Thus, in this research, bone repair capacity of three developed formulations of ceramic scaffolds were evaluated histomorphometrically, after implantation. Scaffolds were based on wollastonite (W) and β-tricalcium phosphate (β-TCP) composites in three different ratios (wt.%). Thirty Wistar rats were randomly assigned to three experimental groups: W-20 (20 W/80β-TCP wt.%), W-60 (60 W/40β-TCP wt.%), and W-80 (80 W/20β-TCP wt.%), evaluated by optical microscopy at biological tests after 15 and 45 days of implantation. Throughout the study, the histological results evidenced that the scaffolds remained at the implantation site, were biocompatible and presented osteogenic potential. The percentage of neoformed mineralized tissue was more evident in the W-20 group (51%), at 45 days. The composite of the W-80 group showed more evident biodegradation than the biomaterials of the W-20 and W-60 groups. Thus, it is concluded that the scaffold containing 20 W/80β-TCP (wt.%) promoted more evident bone formation, but all composites evaluated in this study showed notorious bioactivity and promising characteristics for clinical application.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 58%

Bone regeneration using Wollastonite/β-TCP scaffolds implants in critical bone defect in rat calvaria

Santos¹,

Miguel²,

Barbosa³

et al. 2021

Biomed. Phys. Eng. Express

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Biological behavior of magnesium-substituted hydroxyapatite during bone repair

et al. 2021

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The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI -bone defect filled with HA microspheres; GII -bone defect filled with HA microspheres replaced with Mg; GIII -empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal. Comportamento biológico de hidroxiapatita substituída com magnésio durante o reparo ósseoResumo O objetivo deste estudo foi analisar o comportamento biológico de microesferas de hidroxiapatita (HA) substituída com magnésio (Mg) durante o reparo de defeito ósseo crítico, tendo em vista que este íon é de grande interesse clínico, pois está intimamente associado à homeostasia e à mineralização óssea. Para tanto, utilizou-se 30 ratos para compor três grupos experimentais: GI -defeito ósseo preenchido com microesferas de HA; GII -defeito ósseo preenchido com microesferas de HA substituída com Mg; GIII (controle) -defeito ósseo vazio; avaliados nos pontos biológicos de 15 e 45 dias. Os resultados histológicos evidenciaram, aos 15 dias, discreto infiltrado inflamatório crônico e neoformação óssea restrita às bordas, em todos os grupos. Nos grupos GI e GII, os biomateriais mantiveram-se íntegros e circundados por tecido conjuntivo frouxo. Aos 45 dias, notou-se resposta inflamatória do tipo crônica granulomatosa discreta nos grupos GI e GII, e no GIII presença escassa de células inflamatórias mononucleares. As microesferas implantadas no GI e GII mantiveram-se, em sua maioria, íntegras e envolvidas por tecido conjuntivo fibroso. Notou-se discreta neoformação óssea próxima às bordas e circunjacente a algumas microesferas. No GIII, a mineralização limitou-se às bordas e a área remanescente foi preenchida por tecido conjuntivo fibroso. Conclui-se que os biomateriais foram biocompatíveis, bioativos...

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