Avian Infectious Bronchitis: Virus Distribution in Tissues of Chicks

Hofstad, M. S.; Yoder, Harry W.

doi:10.2307/1588355

Cited by 52 publications

(39 citation statements)

References 2 publications

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“…Following aerosol inoculation of IBV, virus is recovered from the trachea at 24 hr and through 7 or 8 days [8]. In our experiment, IBV antigen was detected in the tracheal epithelium from 1 to 7 d.p.i.…”

Section: Discussionsupporting

confidence: 46%

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Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

Kotani

Shiraishi

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. All stages of degeneration and regeneration in chicken tracheal epithelium were studied morphologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 1 to 7 days post-inoculation (d.p.i.) with a peak on 3 d.p.i. At 1 d.p.i., almost all epithelial cells were involved in the degeneration. At this time, labelling index of bromodeoxyuridine (BrdU) in the basal cells showed significantly high value compared with control. At 2 and 3 d.p.i., a great number of basal cells were recognized, but the BrdU labelling index tended to decrease. At 4 and 5 d.p.i., the BrdU labelling index of basal cells significantly decreased than 1 d.p.i., and a few number of regenerated immature ciliated epithelia appeared. At 6 to 11 d.p.i., the ciliated columnar epithelia increased rapidly in number, and returned to the normal appearance except for non-ciliated patch by 13 d.p.i. These results suggested that the tracheal epithelial cells infected with IBV degenerated within 24 hours and proliferating activity of basal cells functioned immediately, and 3 to 4 days later, these basal cells were differentiated to the ciliated epithelia.-KEY WORDS: bromodeoxyuridine (BrdU), chicken, infectious bronchitis virus, regeneration, trachea.J. Vet. Med. Sci. 62(2): 129-134, 2000 (SPF) White Leghorn chickens (Line LMG) that were kindly provided by a certain breeder in Aburahi of Shiga prefecture were used in this study. Virus: The A1 strain of IBV (IBV-A1) was used in this experiment. This virus was isolated from nephritic kidneys of a 30-day-old chicken of a broiler flock in Hyogo prefecture, and it has been passaged six times in embryonated eggs and twice in chicken kidney cell culture.Experimental infection and autopsy: Thirty-six chickens were inoculated intratrachealy with 0.2 ml of viral inoculum containing 3.2 × 10 3 EID50/0.2 ml of IBV-A1. As controls, three chickens were autopsied before IBV inoculation. Clinical signs were recorded twice a day in the morning and evening. At 1-5, 7, 9, 11, 13, 17 and 21 days postinoculation (d.p.i.), three chickens were killed by pentobarbital anesthesia and the larynx and trachea were immediately removed. One hour before killing, BrdU (5-bromo-2'-deoxyuridine; Sigma Chemical) was injected subcutaneously (100 mg/kg body weight). The dorsal part of the trachea was cut vertically, and the mucous membrane was examined grossly. The tracheal specimens for light microscope (LM), for detection of viral antigen and scanning electron microscope (SEM) were collected in the respective order, 2 cm, 0.5 cm and 0.5 cm in length from the anterior part of trachea.Detection of viral antigen: The viral antigen in trachea was detected by a direct immunofluorescent method. Frozen sections, fixed with cold acetone, were incubated with fluorescein isothiocyanate (FITC)-conjugated chicken anti-IBV antibody in a moist chamber at 4°C overnight and specific fluorescence was examined under a fluorescent Since the respirator...

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Section: Discussionsupporting

confidence: 46%

“…The respiratory system is the primary multiplication site of IB virus (IBV), thereafter the virus distributes to other organs [8]. Death of chicken with IB occurs due to renal failure in the nephrotropic strain [2,26], but tracheal lesion repairs almost completely within 2 or 3 weeks after infection when the infected chicken survived [6,14,16,17,25].…”

mentioning

confidence: 99%

Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

Kotani

Shiraishi

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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“…Many papers have been published on the duration of IBV infections in the chicken (Hofstad & Yoder, 1966;Doherty, 1967;Colwell & Lukert, 1969;Cook, 1971;Crinion et al, 1971;Jones & Jordan, 1972;Hawkes et al, 1983;Smith et al, 1985;ElHouadfi et al, 1986;Jones & Ambali, 1987;Bumstead et al, 1989;Ambali & Jones, 1990;Otsuki et al, 1990;Nakamura et al, 1991;Owen et al, 1991;Bhattacharjee et al, 1995;Dhinakar Raj & Jones, 1997a;De Wit et al, 1998b). These studies involve a large variety of sampled organs, different virus strains, different doses, etc., and can therefore be expected to present partly conflicting data about the intervals in which the virus can be detected in the various organs.…”

Section: Time Between Start Of Infection and Samplingmentioning

confidence: 99%

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

167

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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“…A serial three sections were de-paraffinized, treated by 1% borax, Infectious bronchitis (IB) is an acute and highly contagious viral respiratory disease of chickens. The respiratory system is the primary multiplication site of IB virus (IBV), thereafter the virus distributes to other organs [6]. There have been many reports on the tracheal lesions infected with IBV [3,4,7,10,13,18].…”

Section: Histopathology and Detection Of B-cellsmentioning

confidence: 99%

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Kotani

Wada

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The kinetics of T-cells (CD3 positive (+), CD4+ and CD8+ cells) and B-cells (IgG+, IgM+ and IgA+ cells) in chicken trachea were studied immunohistochemically and histopathologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 16 hr to 6 days post-inoculation (p.i.) with a peak on 4 days p.i. A few IgG+, IgM+ and IgA+ cells were detected in the submucosa from 8 hr p.i. Thereafter IgG+ and IgM+ cells were gradually increased in number, and dramatically increased from 3 days p.i., peaked on 4 days p.i., and gradually decreased after 5 days p.i. IgA+ cells were detected in a small number than IgG+ and IgM+ cells during the all experimental period. These B cells mainly existed in the lamina propria, and some cells were recognized in the interepithelial space. After 14 days p.i., small number of IgG+ and IgM+ cells were detected in the germinal center of lymph follicles in the lamina propria. From 24 to 60 hr p.i., a few number of CD3+, CD4+ and CD8+ cells were detected at the perivascular area in the lamina propria. After 3 or 4 days p.i., each positive T-cells increased rapidly in number, and reached on the peak at 5 days p.i. CD3+, CD4+ and CD8+ cells tend to distribute diffusely, perivascular area, and surrounding area of CD4+ cells, respectively. CD4+ cells were dramatically decreased from 7 days p.i., and CD3+ and CD8+ cells were decreased from 14 days p.i. No T-cells were detected in the lymph follicles in the lamina propria.-KEY WORDS: B lymphocyte, chicken, infectious bronchitis virus, T lymphocyte, trachea.

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Avian Infectious Bronchitis: Virus Distribution in Tissues of Chicks

Cited by 52 publications

References 2 publications

Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

Detection of infectious bronchitis virus

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

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