Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes

Yu, Guanghai; Hai-fen, Peng; Cao, Jun; Liao, Ai‐Mei; Pan, Long; Huang, Jihong; Hui, Ming

doi:10.1007/s11274-021-03191-3

Cited by 13 publications

(5 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For Mel-7, a 40 s exposure emerged as ideal, balancing mutation induction with the preservation of microbial viability. This finding aligns with Liu et al (2020) [32] and Yu et al (2022) [33], highlighting the delicate trade-off between achieving beneficial mutations and avoiding detrimental genetic alterations.…”

Section: Optimization Of Artp Mutation Duration For Enhanced Trait Im...supporting

confidence: 91%

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Zhang,

Lin,

Yan

et al. 2024

Microorganisms

View full text Add to dashboard Cite

This study focuses on optimizing the mutagenesis process for Morchella eximia (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable. Among the screened mutants, strains L21, L23, L44, and L47 exhibited superior growth and high-temperature tolerance, with notable resilience at 30 °C, highlighting their enhanced adaptability to above-optimal temperatures. Furthermore, this research delved into biochemical responses, including lipid peroxidation and non-enzymatic antioxidant content, highlighting the diverse mechanisms, such as enhanced lipid peroxidation resistance and increased antioxidant content, employed by mutant strains to adapt to temperature fluctuations. The activities of antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were shown to be significantly influenced by temperature elevations, illustrating their critical roles in the thermal adaptation of mutant strains. These findings shed light on the importance of considering mutation duration and temperature screening in the development of thermotolerant fungal strains with potential applications in various industries. This study’s breakthrough lies in its comprehensive understanding of the thermal adaptability of Mel-7 mycelia and the identification of promising mutant strains, offering valuable insights for both academic and industrial purposes.

show abstract

Section: Optimization Of Artp Mutation Duration For Enhanced Trait Im...supporting

confidence: 91%

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Zhang,

Lin,

Yan

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…We report a method for screening high-yielding strains of doramectin. Mutagenesis is a cost-effective method for the creation of high-yielding strains, Song et al studied a genetic mutant Streptomyces avermitilis S-233 with high-avermectins B1a by comobtained treatment with carbon heavy ion irradiation and sodium nitrite, and Xu et al obtained a strain Streptomyces viridochromogenes F-23 with high-avilamycin production by combined mutagenesis with UV and ARTP [23,33]. The growth of the strains was inhibited by CHC, which is consistent with literature reports [21].…”

Section: Discussionsupporting

confidence: 72%

“…The growth and metabolism of S. avermitilis are aerobic, and compared to shake flask culture, fermenter culture can better regulate the fermentation process, especially the regulation of dissolved oxygen and replenishment operation. The dissolved oxygen level has a great influence on the production of secondary metabolites during the fermentation of S. avermitilis [33,35,36]. The 30% increase in fermentation yield in doramectin fermenters compared to shake flask fermentation may be caused by the better-dissolved oxygen conditions provided by the fermenters.…”

Section: Discussionmentioning

confidence: 99%

Improved Fermentation Yield of Doramectin from Streptomyces avermitilis N72 by Strain Selection and Glucose Supplementation Strategies

Pan

Cai

2023

Fermentation

View full text Add to dashboard Cite

Doramectin is a macrolide antiparasitic that is widely used in the treatment of mammalian parasitic diseases. Doramectin is usually produced by Streptomyces avermitilis fermentation using cyclohexanecarboxylic acid (CHC) as a precursor; however, the growth of S. avermitilis is usually inhibited by CHC, resulting in a low fermentation yield of doramectin. In this study, a high-yielding strain XY-62 was obtained using the S. avermitilis mutant strain S. avermitilis N72 as the starting strain, then combined with a CHC tolerance screening strategy using ultraviolet and nitrosoguanidine mutagenesis, and a 96 microtiter plate solid-state fermentation primary sieving and shake flask fermentation rescreening method. Compared with S. avermitilis N72, the doramectin fermentation yield increased by more than 1.3 times, and it was more adaptable to temperature, pH, and CHC concentration of the culture; additionally, the viability of the mycelial growth was enhanced. In addition, further studies on the high-yielding strain XY-62 revealed that the accumulation of doramectin could be further increased by glucose supplementation during the fermentation process, and the yield of doramectin reached 1068 μg/mL by scaling up the culture in 50 L fermenters; this has the potential for industrial production. Therefore, mutagenesis combined with CHC tolerance screening is an effective way to enhance the fermentation production of doramectin by S. avermitilis. Our strategy and findings can help to improve the production of doramectin in industrial strains of S. avermitilis.

show abstract

“…According to previous researches (Cai et al, 2021 ; Jiang et al, 2017 ; Liu et al, 2021 ), the lethal rate of 90% is suitable and applicable for ARTP mutation. Therefore, in this study, the treatment time of ARTP mutation was selected as 40 s. In addition, a single ARTP treatment was unusually performed, and this was routinely combined with the optimization of fermentation (Yu et al., 2022a ; Yu et al., 2022b ; Yu et al., 2019b ) or ribosome engineering (Zhang et al., 2019 ) to improve efficiency. Nonetheless, a double-reporter RGMS strategy was recently developed to decrease the frequency of false positives caused by the single-reporter method (Xiang et al., 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection

Jiang

Zhang

Huang

et al. 2022

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

In this study, we employed a reporter-guided mutation selection (RGMS) strategy to improve the rimocidin production of Streptomyces rimosus M527, which is based on a single-reporter plasmid pAN and atmospheric and room temperature plasma (ARTP). In plasmid pAN, PrimA, a native promoter of the loading module of rimocidin biosynthesis (RimA) was chosen as target, and the kanamycin resistance gene (neo) under the control of PrimA was chosen as reporter gene. The integrative plasmid pAN was introduced into the chromosome of S. rimosus M527 by conjugation to yield the initial strain S. rimosus M527-pAN. Subsequently, mutants of M527-pAN were generated by ARTP. Seventy-nine mutants were obtained in total, of which sixty-seven mutants showed higher level of kanamycin resistance (Kanr) than that of the initial strain M527-pAN. The majority of mutants exhibited a slight increase in rimocidin production compared to M527-pAN. Notably, three mutants, M527-pAN-S34, S38, and S52, which exhibited highest kanamycin resistance among all Kanr mutants, showed 34%, 52% and 45% increase in rimocidin production compared to M527-pAN, respectively. Quantitative RT-PCR analysis revealed that the transcriptional levels of neo and rim genes were increased in mutants M527-pAN-S34, S38, and S52 compared to M527-pAN. These results confirmed that the RGMS approach was successful in improving the rimocidin production in S. rimosus M527.

show abstract

Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes

Cited by 13 publications

References 75 publications

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Improved Fermentation Yield of Doramectin from Streptomyces avermitilis N72 by Strain Selection and Glucose Supplementation Strategies

Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection

Contact Info

Product

Resources

About