In situ hybridization mapping and restriction fragment length polymorphism analysis of the porcine albumin (ALB) and transferrin (TF) genes

Abstract

In situ hybridization analyses were conducted on porcine metaphase chromosomes using porcine liver albumin (ALB) and transferrin (TF) cDNA probes. The ALB gene was assigned to the q12 band of chromosome 8 and the TF gene to the q31 band of chromosome 13. For the latter, a statistically significant secondary peak was observed on the 6p15 band. However, the TF probe predominantly hybridized to the 13q31 band, indicating that this band is the most likely site of the TF gene. Since the TF gene belongs to linkage group V, this linkage group can now be assigned to chromosome 13. The TF and ALB probes were also used for restriction fragment length polymorphism (RFLP) analysis. A screening of 10 unrelated animals revealed TaqI RFLPs for both ALB and TF. Family studies indicated that the ALB and TF polymorphisms were controlled by three and two alleles, respectively.

A linkage group on pig chromosome 4 comprising the loci for blood group L, GBA, ATP1B1 and three microsatellites

“…No significant evidence for linkage was obtained between the six markers on chromosome 4 and the 76 other markers scored in this pedigree except that the lod score value for GBA and the microsatellite SO082 just reached statistical significance (Z = 3.0 at 6 = 0.35). This result is in conflict with the assignment of GBA to chromosome 4 since SO082 belongs to a linkage group on chromosome 1 (Ellegren et al 1993). However, the linkage data for GBA and SO082 were considered as a false significance since (1) SO082 did not show a significant lod score to any of the markers flanking GBA on chromosome 4 and (2) the assignment of GBA to chromosome 4 was supported by a far higher lod score (2 =…”

“…The new RFLP markers ATPlBl and GBA were tested for linkage against a set of 80 polymorphic markers previously typed in the pedigree (Ellegren et al 1993; L. Andersson et a]., unpublished).…”

“…In a recent linkage study, based on 56 loci, polymorphic markers were assigned to 13 different autosomal linkage groups in the pig (Ellegren et al 1993). Ten of the linkage groups were assigned to the chromosomes 1, 2, 6, 7 , 8, 9, 12, 13, 14 and 15, respectively, while three were Correspondence: Leif Andersson.…”

Localization of the porcine growth hormone gene to chromosome 12pl.2 p1–5

“…(1992a) recently reported weak evidence (lod score of 3.2) for loose linkage between the K88 loci and EAL, suggesting that the unassigned linkage group U12 is located on chromosome 13. (1992) Lie et al (1987);Xu et al (1992) Kirszenbaum et al (1985); Lie et al (1987); Xu et al (1992) Marklund et al (1992 Archibald et al (1992) Juneja et al (1989); Chaudhary et al (1993); Ellegren et al (1993) Andresen & Jensen (1977; Imlah (1980); Davies et al (1988); Harbitz et al (1990~); Otsu eta]. Since the assignment of EAL to chromo-some 13 is questionable, the EAL, s0097, SLB linkage group is reported here as unassigned.…”

Evidence for linkage between K88ab, K88ac intestinal receptors toEscherichia coliand transferrin loci in pigs

“…The transferrin locus was localized by in situ hybridization on chromosome 13 (Chowdhary et al 1993) The transferrin locus was localized by in situ hybridization on chromosome 13 (Chowdhary et al 1993) …”