Bacillus Calmette-Guerin improves local and systemic response to radiotherapy in invasive bladder cancer

McCormick, Barbara Prack; Belgorosky, Denise; Langle, Yanina; Balarino, Natalia Patricia; Sandes, Eduardo Omar; Eiján, Ana María

doi:10.1016/j.niox.2017.01.008

Cited by 5 publications

(5 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We identified a total of 42 publications [16,19,22,23,30,41,42,45,49,52,56,59,61,64,68,72,74,80,85,89,[100][101][102][103]105,106,[108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123] comparing cell response to chemotherapy and other interventions between 2D and 3D in vitro tumour models, using a variety of anticancer agents and taking into account any publications which involved more than one type of anticancer agents, yielding in total 85 separate findings, including 79 outcomes concerning chemotherapy agents, three involving radiotherapy, and a further five exploring natural compound agents such as orange-peel flavones. The definitions for drug response varied throughout the studies in terms of markers used and duration over which drug responses were measured.…”

Section: Culturing Cancer Cells In 3d Enhances Drug Resistancementioning

confidence: 99%

The Role of Biomimetic Hypoxia on Cancer Cell Behaviour in 3D Models: A Systematic Review

Liu

Mohri

Alsheikh

et al. 2021

Cancers

View full text Add to dashboard Cite

The development of biomimetic, human tissue models is recognized as being an important step for transitioning in vitro research findings to the native in vivo response. Oftentimes, 2D models lack the necessary complexity to truly recapitulate cellular responses. The introduction of physiological features into 3D models informs us of how each component feature alters specific cellular response. We conducted a systematic review of research papers where the focus was the introduction of key biomimetic features into in vitro models of cancer, including 3D culture and hypoxia. We analysed outcomes from these and compiled our findings into distinct groupings to ascertain which biomimetic parameters correlated with specific responses. We found a number of biomimetic features which primed cancer cells to respond in a manner which matched in vivo response.

show abstract

Section: Culturing Cancer Cells In 3d Enhances Drug Resistancementioning

confidence: 99%

The Role of Biomimetic Hypoxia on Cancer Cell Behaviour in 3D Models: A Systematic Review

Liu

Mohri

Alsheikh

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…Ref. [75] Abbreviations: EGFR, epidermal growth factor receptor, HER2, human erbB-2 receptor; HMGB1, high mobility group box 1; PDGF-R, platelet-derived growth factor receptor; PD-L1, programmed death ligand 1; TKI: tyrosine kinase inhibitor.…”

Section: Class Name Targetmentioning

confidence: 99%

“…Bacillus Calmette-Guérin (BCG) bladder instillation is commonly used after local tumour resection for patients with superficial bladder cancer. In addition, BCG bacteria-induced immune response has been studied in BCa to improve response to RT [ 75 ]. Invasive murine BCa cell line MB49-I was cultured in monolayers in 2D, in spheroids in vitro in 3D and inoculated in vivo in the syngeneic mice.…”

Section: Targeted Agents To Improve Rt Response In Bcamentioning

confidence: 99%

See 1 more Smart Citation

Review of Experimental Studies to Improve Radiotherapy Response in Bladder Cancer: Comments and Perspectives

Silina

Maksut

Bernard‐Pierrot

et al. 2020

Cancers

View full text Add to dashboard Cite

Bladder cancer is among the top ten most common cancer types in the world. Around 25% of all cases are muscle-invasive bladder cancer, for which the gold standard treatment in the absence of metastasis is the cystectomy. In recent years, trimodality treatment associating maximal transurethral resection and radiotherapy combined with concurrent chemotherapy is increasingly used as an organ-preserving alternative. However, the use of this treatment is still limited by the lack of biomarkers predicting tumour response and by a lack of targeted radiosensitising drugs that can improve the therapeutic index, especially by limiting side effects such as bladder fibrosis. In order to improve the bladder-preserving treatment, experimental studies addressing these main issues ought to be considered (both in vitro and in vivo studies). Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for systematic reviews, we conducted a literature search in PubMed on experimental studies investigating how to improve bladder cancer radiotherapy with different radiosensitising agents using a comprehensive search string. We made comments on experimental model selection, experimental design and results, formulating the gaps of knowledge still existing: such as the lack of reliable predictive biomarkers of tumour response to chemoradiation according to the molecular tumour subtype and lack of efficient radiosensitising agents specifically targeting bladder tumour cells. We provided guidance to improve forthcoming studies, such as taking into account molecular characteristics of the preclinical models and highlighted the value of using patient-derived xenografts as well as syngeneic models. Finally, this review could be a useful tool to set up new radiation-based combined treatments with an improved therapeutic index that is needed for bladder preservation.

show abstract

“…Sb (400 mg/kg) or equivalent DMSO dissolved in 0.1 mL of cell medium were administered by gavage five days/week, adapted from Yan et al 21 RT (18 Gy) was administered as six fractions of 3 Gy, being three fractions/week (Figure 5A). RT was performed as previously, 22 mice were anesthetized with ketamine (70 mg/ kg), and xylazine (5 mg/kg) and placed on paraffin stretchers with acrylic lids so that only tumors remained in the irradiation field (Figure 5B). Tumor volumes were calculated as 0.4 × width 2 × length.…”

Section: Tumor Growth In Vivomentioning

confidence: 99%

Flavonoid silybin improves the response to radiotherapy in invasive bladder cancer

McCormick

Langle

Belgorosky

et al. 2018

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Conservative treatment for invasive bladder cancer (BC) involves a complete transurethral tumor resection combined with chemotherapy (CT) and radiotherapy (RT). The major obstacles of chemo-radiotherapy are the addition of the toxicities of RT and CT, and the recurrence due to RT and CT resistances. The flavonoid Silybin (Sb) inhibits pathways involved in cell survival and resistance mechanisms, therefore the purpose of this paper was to study in vitro and in vivo, the ability of Sb to improve the response to RT, in two murine BC cell lines, with different levels of invasiveness, placing emphasis on radio-sensitivity, and pathways involved in radio-resistance and survival. In vitro, Sb radio-sensitized murine invasive cells through the inhibition of RT-induced NF-κB and PI3K pathways, and the increase of oxidative stress, while non-invasive cells did not show to be sensitized. In vivo, Sb improved RT-response and overall survival in invasive murine tumors. As Sb is already being tested in clinical trials for other urological cancers and it improves RT-response in invasive BC, these results could have translational relevance, supporting further research.

show abstract

Bacillus Calmette-Guerin improves local and systemic response to radiotherapy in invasive bladder cancer

Cited by 5 publications

References 32 publications

The Role of Biomimetic Hypoxia on Cancer Cell Behaviour in 3D Models: A Systematic Review

The Role of Biomimetic Hypoxia on Cancer Cell Behaviour in 3D Models: A Systematic Review

Review of Experimental Studies to Improve Radiotherapy Response in Bladder Cancer: Comments and Perspectives

Flavonoid silybin improves the response to radiotherapy in invasive bladder cancer

Contact Info

Product

Resources

About