Bacillus cereus strain isolated from Demodex folliculorum in patients with topical steroid-induced rosaceiform facial dermatitis

Abstract

The aim of the study was to identify Bacillus species from the Demodex folliculorum of patients with topical steroidinduced facial rosaceiform dermatitis. Of the 75 patients examined, 20% had clinical spinulosis, while 18.66% had dermoscopic features of Demodex: follicular plugs and tails. Of the 17.33% positive patients identified upon microscopy for Demodex, samples for bacterial culture were plated on trypticase soy Colombia agar. Identification was performed by microorganisms grown method mass spectrometry. We identified a strain of Bacillus cereus.

Rosacea‐like demodicosis (but not primary demodicosis) and papulopustular rosacea may be two phenotypes of the same disease – a microbioma, therapeutic and diagnostic tools perspective

“…2 Other endosymbionts described as related to Demodex are as follows: (i) Bacillus simplex, which was isolated from D. folliculorum in a patient with primary demodicosis, 3 (ii) Bacillus pumilus-positive culture and mass spectrometry were found in a patient with rosacea and D. folliculorum 4 and (iii) Bacillus cereus, instead of B. oleronius, was identified in a patient with secondary demodicosis associated with steroid-induced rosacea-like facial dermatitis in one positive culture. 5 Would they care to comment on this?…”

Undesirable Effects of Some Topical Antiseptics Chemical, pharmacological and dermatological aspects

“…Antiseptics are used in various medical and surgical conditions [64][65][66][67][68][69][70][71][72]. Their role is to inhibit the bacterian,viral pathologic proliferation but also they act by inhibing normal microbiota or endosymbionts [73][74][75][76][77][78][79][80][81][82][83]. It is useful to inform the parents of the children about the possible adverse reactions and the informed consent should be signed by them [84,85].…”

Reply to: Kubiak K et al. Endosymbiosis and its significance in dermatology

“…5 The difference of microbiome between demodicosis, SIRLFD and rosacea needs more and larger future studies for possible explanations of endosymbiosis between Bacillus species and D. folliculorum. 6 Recently, Demodex was proposed as a delivery tool for topical targeted medications in the skin and for medications with cytotoxic properties directed to early non-melanoma skin cancer or melanoma. 7 After reviewing the literature, we also intend to add some data, notes and comments about the authors subtitle tick-borne diseases.…”

“…Nowadays, the concept of secondary barrier dysfunction has emerged, including the concept of a disturbed epidermal barrier function in rosacea, measured only in skin areas affected by inflammation. 1,2,[4][5][6][7][8][9][10][11] Barrier impairment may lead to irritation and a facilitated allergen sensibilization. Contact allergies contribute to the progression of the skin disease.…”

“…Contact allergies contribute to the progression of the skin disease. 6,[12][13][14][15][16] We performed a retrospective cohort study on a total of 143 rosacea patients who were patch-tested at the Allergology Outpatient Unit of the Department of Dermatology, Venerology and Dermatooncology of Semmelweis University. Testing was performed for suspected contact allergy based on therapy resistance or on the patients' own observations that some topical medication or cosmetics had worsened the skin lesions.…”